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公开(公告)号：US08129509B2
公开(公告)日：2012-03-06
申请号：US10413942
申请日：2003-04-15
CPC分类号： G01N33/532 , C07H21/00
摘要： Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
摘要翻译： 提供了化合物和方法用于将标记物与分子的单步共价连接，包括形成用于烷基化分子的共价连接的标记试剂。 然后，在标记试剂与分子具有反应性，从而形成共价键的条件下，将可共价连接的标记试剂与含有该分子的混合物结合。
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公开(公告)号：US07045356B2
公开(公告)日：2006-05-16
申请号：US10795679
申请日：2004-03-08
申请人： Vladimir Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
发明人： Vladimir Trubetskoy , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , So Chun Wong , Jason Klein
IPC分类号： C12N15/88
CPC分类号： A61K48/0008
摘要： We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
摘要翻译： 我们描述了一种产生多层颗粒的方法，包括将聚合物与带相反电荷的聚合物缩合形成颗粒，并依次向形成至少三层聚合物的颗粒中加入带相反电荷的聚合物。 该方法用于形成用于将生物活性化合物递送至细胞的组合物。
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公开(公告)号：US07022525B2
公开(公告)日：2006-04-04
申请号：US10755785
申请日：2004-01-12
申请人： Vladimir Trubetskoy , Jon A. Wolff , Paul M. Slattum , Lisa Hanson , Vladimir G. Budker , James E. Hagstrom
发明人： Vladimir Trubetskoy , Jon A. Wolff , Paul M. Slattum , Lisa Hanson , Vladimir G. Budker , James E. Hagstrom
IPC分类号： C12N15/09 , C12N15/00 , C12N15/11 , A61K48/00 , A61K39/395
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US6126964A
公开(公告)日：2000-10-03
申请号：US778657
申请日：1997-01-03
申请人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
发明人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
CPC分类号： A61K47/48315 , A61K47/48192 , A61K47/48215 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
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公开(公告)号：US07476401B2
公开(公告)日：2009-01-13
申请号：US11248993
申请日：2005-10-12
申请人： Sean D. Monahan , Vladimir G. Budker , Kirk Ekena , Lisa Nader
发明人： Sean D. Monahan , Vladimir G. Budker , Kirk Ekena , Lisa Nader
CPC分类号： A61K9/127 , A61K47/22 , A61K47/24 , A61K47/51 , A61K47/543 , A61K47/6949 , B82Y5/00
摘要： Compositions and methods for delivery of proteins and peptides to mammalian cells in vitro are described. Specifically, polypeptide-surfactant complexes formed from noncovalent hydrophobation of polypeptides and reversible hydrophobic modification of polypeptides are described. The compositions can be used to delivery positively charged, negatively charged and charge neutral polypeptides to cells.
摘要翻译： 描述了用于在体外将哺乳动物细胞递送蛋白质和肽的组合物和方法。 具体地，描述了由多肽的非共价疏水化和多肽的可逆疏水修饰形成的多肽 - 表面活性剂复合物。 该组合物可用于将带正电荷的带负电荷的中性多肽递送至细胞。
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46.发明申请Reversible Hydrophobic Modification of Drugs for Improved Delivery to Cells 审中-公开用于改善细胞递送的药物的可逆疏水改性公开(公告)号：US20090074885A1
公开(公告)日：2009-03-19
申请号：US12260679
申请日：2008-10-29
IPC分类号： A61K31/704 , A61K33/24 , A61K31/337 , A61P35/04 , A61K31/195
CPC分类号： A61K31/58 , A61K9/0019 , A61K31/195 , A61K31/337 , A61K31/695 , A61K31/704 , A61K47/54 , A61K47/542 , C07F7/10 , C07F7/1804 , C07J1/00
摘要： Described are drug formulations that increase regional delivery of the drugs to cells. Methods for reversibly increasing the hydrophobicity of a drug through hydrolytically labile attachment of a hydrophobic moiety and methods for delivering the modified drug to cells are described. Hydrophobic modification increases drug delivery, while lability minimizes entry of the drug into non-target cells.
摘要翻译： 描述了增加药物对细胞的区域递送的药物制剂。 描述了通过疏水部分的水解不稳定连接可逆地增加药物的疏水性的方法和将修饰的药物递送至细胞的方法。 疏水性修饰增加药物递送，同时使药物最小化进入非靶细胞。
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公开(公告)号：US07396821B1
公开(公告)日：2008-07-08
申请号：US09707117
申请日：2000-11-06
申请人： Jon A. Wolff , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir G. Budker
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permability of the vessel is increased using an exterior cuff and immunosuppression is applied thereby the genetic material is delivered to the parenchymal cell.
摘要翻译： 公开了将遗传物质转染入哺乳动物细胞以改变细胞的内源性质的方法。 该方法包括设计用于转染的多核苷酸。 然后将多核苷酸插入哺乳动物血管中。 在插入之前，插入之后或插入同时，使用外部袖口增加血管的渗透性，并施加免疫抑制，从而将遗传物质递送至实质细胞。
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公开(公告)号：US07435723B2
公开(公告)日：2008-10-14
申请号：US10680742
申请日：2003-10-06
申请人： James E. Hagstrom , Mark Noble , Julia Hegge , Vladimir G. Budker
发明人： James E. Hagstrom , Mark Noble , Julia Hegge , Vladimir G. Budker
CPC分类号： C07H21/00 , A61K47/62 , A61K47/645 , A61K48/00 , C12N15/87
摘要： Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.
摘要翻译： 公开了一种使用血管内施用途径向哺乳动物前列腺细胞体内递送多核苷酸的系统。 将多核苷酸插入到哺乳动物脉管系统的注射溶液中。 以适当的速率插入注射溶液会增加组织中血管外液体的体积，从而便于将多核苷酸递送至细胞。
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公开(公告)号：US07507722B1
公开(公告)日：2009-03-24
申请号：US09707000
申请日：2000-11-06
申请人： Jon A. Wolff , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir G. Budker
IPC分类号： A01K43/04 , A61K31/715
CPC分类号： A61K31/715 , C12N15/89
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased using an exterior cuff thereby the genetic material is delivered to the parenchymal cell.
摘要翻译： 公开了将遗传物质转染入哺乳动物细胞以改变细胞的内源性质的方法。 该方法包括设计用于转染的多核苷酸。 然后将多核苷酸插入哺乳动物血管。 在插入之前，插入之后或插入同时，使用外部袖带增加血管的渗透性，从而将遗传物质递送至实质细胞。
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50.发明授权Devices and processes for distribution of genetic material to mammalian limb 有权将遗传物质分配给哺乳动物肢体的装置和方法公开(公告)号：US07642248B2
公开(公告)日：2010-01-05
申请号：US11694140
申请日：2007-03-30
申请人： Vladimir G. Budker , Jon A. Wolff
发明人： Vladimir G. Budker , Jon A. Wolff
IPC分类号： A61K31/70
CPC分类号： A61K48/0075
摘要： A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from Muscular Dystrophy. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
摘要翻译： 描述了将治疗性多核苷酸递送至患有或可能患有肌营养不良症的肢体肌肉组织的方法。 将多核苷酸插入哺乳动物肢体血管如动脉。 通过将含有多核苷酸的溶液的注入与使用外部应用的袖带组合来提高递送效率和分布。
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