公开(公告)号：US5037958A

公开(公告)日：1991-08-06

申请号：US146167

申请日：1987-12-12

申请人： Yoshiyuki Hashimoto ,  Kenji Chiba ,  Hirotsugu Komatsu ,  Takeki Okumoto

发明人： Yoshiyuki Hashimoto ,  Kenji Chiba ,  Hirotsugu Komatsu ,  Takeki Okumoto

IPC分类号： C12P21/00 ,  A61K35/12 ,  A61K38/00 ,  A61P37/06 ,  C07K14/005 ,  C07K14/195 ,  C07K14/52 ,  C12R1/91

CPC分类号： A61K38/1709 ,  A61K35/17 ,  Y10S530/827 ,  Y10S530/829

摘要： A new immunosuppressive factor derived from human T cell leukemia cells characterized by the following properties:(1) molecular weight: 45,000 to 65,000 daltons and 150,000 to 200,000 daltons by gel filtration, and approximately 31,000 daltons by SDS-polyacrylamide gel electrophoresis;(2) isoelectric point: 4.6 to 4.8;(3) being elutable at a concentration of 0.31 to 0.32 M sodium chloride by FPLC-Mono Q anion exchange chromatography;(4) not adsorbable to immobilized concanavalin A Sepharose or blue Sepharose;(5) resistant to deoxyribonuclease, ribonuclease, papin and periodic acid but sensititive to trypsin, .alpha.-chymotrypsin or pronase;(6) stable at pH 2 to 10;(7) stable for a long time at 4.degree. C. but partially inactivated by heat treatment at 56.degree. C. or 90.degree. C. for 30 minutes;(8) not inhibitable by 2-mercaptoethanol, levamisole, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, .alpha.-methyl-D-mannoside, L-arginine or L-ornithine;(9) suppressing production of antibody;(10) suppressing blastogeneis of lymphocytes;(11) suppressing cell proliferation; and(12) having structural amino acid and specific contents thereof.This immunosuppressive factor has an excellent immunosuppressing effect and is useful as medicine or reagent.

公开(公告)号：US4741195A

公开(公告)日：1988-05-03

申请号：US905326

申请日：1986-09-09

申请人： Toshihiko Arai ,  Masaharu Kusunoki ,  Tadao Odaka ,  Shozo Imanishi ,  Yoshinori Kato ,  Masakazu Hashimoto ,  Yoshiyuki Hashimoto

发明人： Toshihiko Arai ,  Masaharu Kusunoki ,  Tadao Odaka ,  Shozo Imanishi ,  Yoshinori Kato ,  Masakazu Hashimoto ,  Yoshiyuki Hashimoto

IPC分类号： B30B15/00 ,  B21D43/05 ,  B30B1/24 ,  B21D43/10

CPC分类号： B21D43/055 ,  Y10T74/165

摘要： In a transfer press having feed bars and a press drive, an improved feed bar driving apparatus has a vertical rod reciprocally movable in association with the press drive and having a threaded portion, a drive means to the vertical rod means for rotating the vertical rod means in opposite directions, a feed screw device connected to the threaded portion of the vertical rod for being rotated by the vertical rod in opposite directions by rotation and reciprocation of the vertical rod, a pair of feed bar receptacles slidably supporting the feed bars and connected to the feed screw device for being moved toward and away from each other when the feed screw device is rotated in respective opposite directions for moving the feed bars in a clamping and unclamping movement, a cam rotatable in assocation with the press drive, a lever mounted on a fulcrum and connected to the vertical rod and in contact with the cam for being pivoted around the fulcrum by the cam for moving the vertical rod through a stroke, and a stroke adjusting mechanism connected to the fulcrum for changing the position of the fulcrum for selectively adjusting the stroke of the vertical rod and thereby adjusting the stroke of the feed bars.

摘要翻译： 在具有进给杆和压力传动装置的转印机中，改进的进给杆驱动装置具有可与压力传动相关联地往复运动并具有螺纹部分的垂直杆，垂直杆装置的驱动装置，用于使垂直杆装置 在相反方向上，连接到垂直杆的螺纹部分的进给螺杆装置，用于垂直杆通过垂直杆的旋转和往复运动而沿相反方向旋转，一对进给杆接收器，可滑动地支撑进给杆并连接到 所述进给螺杆装置在进给螺旋装置沿相反方向旋转以便在夹紧和松开运动中移动进给杆时相互移动，可与压力传动相关联地旋转的凸轮，安装在 支点并连接到垂直杆并且与凸轮相接触，以便通过凸轮围绕支点枢转，用于移动竖杆杆 以及与该支点连接的行程调节机构，用于改变支点的位置，以便选择性地调整垂直杆的行程，从而调整进给杆的行程。

公开(公告)号：US4645667A

公开(公告)日：1987-02-24

申请号：US566074

申请日：1983-12-27

申请人： Yoshiyuki Hashimoto ,  Tomohiro Toida ,  Kazunori Sekine ,  Minoru Saito ,  Takuji Kawashima ,  Morio Kuboyama

发明人： Yoshiyuki Hashimoto ,  Tomohiro Toida ,  Kazunori Sekine ,  Minoru Saito ,  Takuji Kawashima ,  Morio Kuboyama

IPC分类号： A61K35/74 ,  A61P35/00 ,  C12P1/04 ,  A61K39/02 ,  A61K35/78

CPC分类号： C12P1/04

摘要： This invention relates to an antitumor agent comprising as a main ingredient a chemically purified cell walls of microorganisms belonging to genus Bificobacterium having physical integrity of cell wall structure thereof, obtained without being subjected to chemical and/or physical destruction of said cell wall during removal of intracellular substances of said cell, and a process for manufacturing the same, which comprises the steps of; (a) treating said cells with surfactants, (b) treating said cells with proteases, nucleases, organic solvents and dilute acid, to remove intracellular substances out of said cells without destruction thereof, and (c) separating a chemically purified cell walls having physical integrity of cell wall structure thereof.

摘要翻译： 本发明涉及一种抗肿瘤剂，其包含作为主要成分的化学纯化的细胞壁，所述微生物属于具有细胞壁结构的物理完整性的微生物，其细胞壁结构具有物理完整性，而不会在除去所述细胞壁时被化学和/或物理破坏所述细胞壁 所述细胞的细胞内物质及其制造方法，包括以下步骤： （a）用表面活性剂处理所述细胞，（b）用蛋白酶，核酸酶，有机溶剂和稀酸处理所述细胞，以除去所述细胞中的细胞内物质而不破坏细胞，和（c）分离具有物理 细胞壁结构的完整性。