公开(公告)号：US20030091475A1

公开(公告)日：2003-05-15

申请号：US10260704

申请日：2002-09-26

申请人： Micralyne Inc.

发明人： Pengguang Yu ,  Kevin E. Kornelsen

IPC分类号： B01L003/00 ,  G01N021/64

CPC分类号： B01L3/502761 ,  B01D35/10 ,  B01J19/0046 ,  B01J2219/00414 ,  B01J2219/00461 ,  B01J2219/00463 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L2200/0647 ,  B01L2400/0403 ,  B01L2400/086

摘要： A bead trapping device for trapping beads. The device is formed from a substrate, posts extending from the substrate; the posts being patterned to form cavities between the posts, the cavities having a diameter between 0.5 nullm and 200 nullm; and means for loading beads onto the substrate. A method of trapping microscopic beads for analysis, the method comprising the steps of contacting a fluid containing beads with a substrate having posts extending from the substrate to form bead trapping cavities, the bead trapping cavities having a diameter between 0.5 nullm and 200 nullm; and loading the beads into the bead trapping cavities.

摘要翻译： 用于捕获珠的珠捕获装置。 该装置由基板形成，从基板延伸的柱; 柱被图案化以在柱之间形成空腔，空腔的直径在0.5μm和200μm之间; 以及用于将珠粒加载到基底上的装置。 一种捕获显微珠用于分析的方法，所述方法包括以下步骤：使含有珠粒的流体与具有从所述基底延伸的柱的基底接触以形成珠粒捕获腔，所述珠捕获腔的直径在0.5μm和200μm之间; 并将珠子装载到珠粒捕获腔中。

公开(公告)号：US20030081204A1

公开(公告)日：2003-05-01

申请号：US10226592

申请日：2002-08-23

发明人： Paul J. Cronin ,  Peter J. Miller

IPC分类号： G01J003/42 ,  G01J003/00 ,  G01N021/64

CPC分类号： G01N21/645 ,  G01N21/31 ,  G01N2021/6421

摘要： A method is described that includes measuring, at each of a set of W wavelength bins, a spectral response of at least one region of a sample stained with multiple stains, and determining the concentration of at least one of the stains in the region of the sample based in part on the spectral responses, the wavelength bins being chosen so that a matrix of elements that represent the responses of the stains at the wavelength bin has an inverse for which a mathematical stability is maximum relative to the inverses of other matrices of elements that represent the responses of the stains for other possible sets of wavelength bins.

摘要翻译： 描述了一种方法，其包括在一组W波长区段中的每一个处测量被多个污渍染色的样品的至少一个区域的光谱响应，以及确定在多个污渍区域中的至少一个污渍的浓度 样品部分地基于光谱响应，选择波长区，使得表示波长箱上的污点的响应的元素矩阵具有相对于元素的其它矩阵的逆的数学稳定性最大的逆矩阵 其代表着对于其它可能的波长箱组的污渍的响应。

公开(公告)号：US20030071227A1

公开(公告)日：2003-04-17

申请号：US10051205

申请日：2002-01-16

发明人： Ralf Wolleschensky

IPC分类号： G01N021/64

CPC分类号： G01J3/2803 ,  G01N21/64 ,  G02B21/002

摘要： A method for the optical acquisition of characteristic quantities of an illuminated specimen, wherein a signal that is backscattered, reflected and/or fluoresced and/or transmitted from the specimen comprises the steps of detecting the signal by a spatially resolving detector in a plurality of channels in that the radiation coming from the specimen is imaged on the detector so as to be spectrally split and combining detection channels so that the quantity of measurements that are read out and further processed is less than the number of detection channels.

摘要翻译： 一种用于光学获取照明样本的特征量的方法，其中从样本反向散射，反射和/或发射和/或发射的信号包括以下步骤：通过多个通道中的空间分辨检测器检测信号 因为来自样本的辐射被成像在检测器上，以便被光谱分割并组合检测通道，使得读出和进一步处理的测量量小于检测通道的数量。

公开(公告)号：US20030071226A1

公开(公告)日：2003-04-17

申请号：US10268159

申请日：2002-10-09

申请人： Leica Microsystems Heidelberg GmbH

发明人： Johann Engelhardt ,  Juergen Hoffmann

IPC分类号： G01N021/64

CPC分类号： G01N21/6458

摘要： The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.

摘要翻译： 本发明公开了一种荧光显微镜，包括发射用于照射样本的激发光的光源，用于定义用于激发和检测波长的二维搜索区域的装置，用于从搜索区域选择次区域的装置，至少一个检测器 检测从样本进行的检测光，以及显示器，用于显示至少一部分样本的图像。 此外，本发明公开了一种荧光显微镜的方法。

公开(公告)号：US20030052281A1

公开(公告)日：2003-03-20

申请号：US10137983

申请日：2002-04-30

发明人： Daniel J. Rader ,  John R. Torczynski ,  Karl Wally ,  John E. Brockmann

IPC分类号： G01N021/64

CPC分类号： G01N1/2202 ,  G01N1/24 ,  G01N1/405 ,  G01N2001/2223

摘要： An aerosol lab-on-a-chip (ALOC) integrates one or more of a variety of particle collection, classification, concentration (enrichment), and characterization processes onto a single substrate or layered stack of such substrates. By mounting a UV laser diode laser light source on the substrate, or substrate stack, so that it is located down-stream of the sample inlet port and at right angle to the sample particle stream, the UV light source can illuminate individual particles in the stream to induce a fluorescence response in those particles having a fluorescent signature such as biological particles. some of said particles. A illuminated particle having a fluorescent signal above a threshold signal would trigger a sorter module that would separate that particle from the particle stream.

摘要翻译： 气溶胶实验室（ALOC）将一种或多种各种颗粒收集，分级，浓缩（浓缩）和表征过程集成到单个基板或这种基板的分层堆叠上。 通过将UV激光二极管激光光源安装在基板或基板堆叠上，使得其位于样品入口的下游并与样品颗粒流成直角，UV光源可以照亮样品颗粒流中的单个颗粒 流以在具有荧光特征的那些颗粒中产生荧光响应，例如生物颗粒。 一些所述颗粒。 具有高于阈值信号的荧光信号的照明粒子将触发将粒子与粒子流分离的分选器模块。

公开(公告)号：US20030042428A1

公开(公告)日：2003-03-06

申请号：US10206824

申请日：2002-07-29

申请人： Berthold Technologies GmbH & Co. KG

发明人： Michael Peukert ,  Nortbert Klaiber ,  Berthold Breitkopf

IPC分类号： G01N021/64

CPC分类号： G01N21/6452 ,  G01N21/76 ,  G01N2021/6471

摘要： An apparatus for selected measurement of in particular luminescent and/or fluorescent radiation has a plurality of measurement light paths (M1, M2, M3), which each have their own measuring devices optimized for the particular measurement mode. As a common device, a filter holder (5) is used, in which the detector and each of the measuring devices have access to each of the openings of the filter holder, and each of these openings can be equipped with an emissions filter that is expedient for the particular measurement mode involved. The result is an especially compact arrangement of a multipurpose measuring instrument with measurement properties that is largely equivalent to the measurement properties of special individual measuring instruments and that is also suitable in particular as an individual instrument for special luminescence measurements, such as BRET measurements.

摘要翻译： 用于特别是发光和/或荧光辐射的选择测量的装置具有多个测量光路（M1，M2，M3），它们各自具有针对特定测量模式而优化的测量装置。 作为通用装置，使用过滤器保持器（5），其中检测器和每个测量装置可以进入过滤器保持器的每个开口，并且这些开口中的每一个可以配备有排放过滤器 适用于所涉及的特定测量模式。 结果是具有测量性质的多用途测量仪器的特别紧凑的布置，其大体上等同于特殊的单独测量仪器的测量特性，并且特别适用于特殊发光测量（例如BRET测量）的单独仪器。

公开(公告)号：US20030020022A1

公开(公告)日：2003-01-30

申请号：US10188616

申请日：2002-07-03

发明人： Susumu Kuwabata ,  Eiichiro Fukusaki ,  Motonao Nakao

IPC分类号： G01N021/64

CPC分类号： G01N21/6489 ,  G01N21/66 ,  G01N2021/6421 ,  G01N2021/6471

摘要： A compact and low-cost fluorescence reading apparatus capable of detecting multiple wavelengths is provided. The apparatus employs a semiconductor nanoparticle (201) as a fluorescent substance so as to enable a plurality of kinds of fluorescent substances to be simultaneously excited by a single excitation light source (220). Alternatively, the plurality of kinds of fluorescent substances are simultaneously excited by the application of a voltage.

摘要翻译： 提供了能够检测多个波长的紧凑且低成本的荧光读取装置。 该装置采用半导体纳米颗粒（201）作为荧光物质，以使多种荧光物质能够被单个激发光源（220）同时激发。 或者，通过施加电压同时激发多种荧光物质。

公开(公告)号：US20030010931A1

公开(公告)日：2003-01-16

申请号：US09906362

申请日：2001-07-16

发明人： Richard J. Pittaro

IPC分类号： G01N021/64

CPC分类号： G01N21/64

摘要： Methods and systems are disclosed for analyzing signals that exhibit time decay such as luminescent signals. The signal is detected using at least two predetermined integration periods that are offset by ninety degrees with respect to a fundamental excitation frequency. The characteristics of the signal are determined by processing the signals detected during the at least two integration periods. A surface comprising a plurality of signal producing features, such as an array of such features, may be analyzed in accordance with the present invention. Relatively inexpensive area sensor detectors may be employed in the analysis of such arrays.

摘要翻译： 公开了用于分析显示诸如发光信号之类的时间衰减的信号的方法和系统。 使用相对于基本激励频率偏移九十度的至少两个预定积分周期来检测信号。 通过处理在至少两个积分周期期间检测到的信号来确定信号的特性。 可以根据本发明分析包括多个信号产生特征的表面，例如这种特征的阵列。 相对便宜的区域传感器检测器可以用于这种阵列的分析。

公开(公告)号：US20020185608A1

公开(公告)日：2002-12-12

申请号：US10158909

申请日：2002-06-03

发明人： Manfred Wieser

IPC分类号： G01N021/64

CPC分类号： G01J3/4406 ,  G01J3/1256 ,  G01J3/42 ,  G01N21/253 ,  G01N21/645 ,  G01N2021/6482

摘要： The invention relates to a method and measuring device for determining at least one luminescence, fluorescence, or absorption parameter of a sample, including an excitation light source supplying the excitation radiation directed onto the sample, a detector unit for detection of the emission radiation (i.e., the radiation to be measured) given off by the sample, and an acousto-optical tunable filter (AOTF) being provided in the radiation path of the excitation radiation and/or in the radiation path of the emission radiation. The ultrasonic oscillations of the acousto-optical filter are excited by a high-frequency oscillator, which is furnished with a unit for amplitude modulation in the form of a rectangular, Gaussian, Hann, or Hamming window, thus providing an optical switch with switching times in the nullsec range.

摘要翻译： 本发明涉及一种用于确定样品的至少一种发光，荧光或吸收参数的方法和测量装置，包括提供引导到样品上的激发辐射的激发光源，用于检测发射辐射的检测器单元（即 ，待测量的辐射），以及在激发辐射的辐射路径和/或发射辐射的辐射路径中提供的声光可调谐滤波器（AOTF）。 声光滤波器的超声波振荡由高频振荡器激励，高频振荡器配备有以矩形，高斯，汉斯或汉明窗形式进行幅度调制的单元，从而提供具有切换时间的光学开关 在音乐范围内。

公开(公告)号：US20020167662A1

公开(公告)日：2002-11-14

申请号：US10098534

申请日：2002-03-18

申请人： Yokogawa Electric Corporation

发明人： Takeo Tanaami ,  Yumiko Sugiyama

IPC分类号： G01N021/64

CPC分类号： G02B21/0076 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6421 ,  G01N2021/6439 ,  G02B21/0028

摘要： The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

摘要翻译： 本发明提供了一种生物芯片阅读器，用于通过用激发光照射相关的光束（例如激光）作为激发光，将具有荧光物质被粘附的基因的荧光图像信息和被注入生物芯片单元的荧光图像信息读出。 本发明旨在实现小而便宜且高度耐用的生物芯片读取器。