公开(公告)号：US20230241571A1

公开(公告)日：2023-08-03

申请号：US18010424

申请日：2021-06-14

Applicant: DNA Script

Inventor： Carl Martin ,  Marc Artigue ,  Sandrine Creton ,  Gabriel de Crozals ,  Xavier Godron ,  Adrian Horgan ,  Henri Lachaize ,  John Lucky ,  Christine Peponnet ,  Thomas Ybert

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00452 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00722

Abstract: The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

公开(公告)号：US09862918B2

公开(公告)日：2018-01-09

申请号：US14372202

申请日：2012-01-19

Applicant: Saburo Ito

Inventor： Saburo Ito

IPC: C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M1/32 ,  B01L3/00 ,  G01N35/10 ,  B01L3/02

CPC classification number: C12M23/12 ,  B01J2219/00315 ,  B01J2219/00414 ,  B01J2219/00468 ,  B01L3/021 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  G01N35/1011

Abstract: A well plate is formed with a well for holding a subject to be sucked by a suction nozzle on an inner bottom part and storing liquid and, a clearance forming member for forming a clearance to allow the liquid to flow in a state where a tip part of the suction nozzle is inserted into and held in contact with the well is provided in the well. According to the present invention, the clearance enabling the liquid to flow is formed even in the state where the tip part of the suction nozzle is inserted into and held in contact with the well in sucking the subject held in the well by the suction nozzle. The suction nozzle can suck the liquid around through the clearance and the subject held in the well is efficiently sucked through the suction port along the flow of the sucked liquid.

公开(公告)号：US20160339427A1

公开(公告)日：2016-11-24

申请号：US15114894

申请日：2015-01-28

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Peter Wiktor

IPC: B01L3/00 ,  B01J19/00

CPC classification number: B01L3/50853 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00396 ,  B01J2219/00414 ,  B01J2219/00416 ,  B01J2219/00418 ,  B01L3/0282 ,  B01L3/0293 ,  B01L3/50851 ,  B01L2200/025 ,  B01L2200/0642 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/046 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0605 ,  B01L2400/0666 ,  B01L2400/0683 ,  B01L2400/0688

Abstract: A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.

Abstract translation: 提供了一种用于密封微反应器阵列的微反应器中的试剂的微反应器阵列平台和方法。 微反应器阵列平台包括微反应器阵列和具有第一表面和相对的第二表面的密封膜，密封膜构造成可移动地密封微反应器阵列。 微反应器阵列平台还包括用于经由阵列和密封膜的第二表面之间的流体路径将试剂输送到微反应器阵列中的注射器，以及用于将密封液引导到密封膜的第一表面上的施加器。 微反应器阵列平台还包括用于在喷射器中的试剂和微反应器阵列与密封膜的第二表面之间的空间之间产生压力差的系统。

公开(公告)号：US08658112B2

公开(公告)日：2014-02-25

申请号：US13286418

申请日：2011-11-01

Applicant: Arkadij M. Elizarov ,  Hartmuth C. Kolb ,  R. Michael Van Dam ,  James R. Heath

Inventor： Arkadij M. Elizarov ,  Hartmuth C. Kolb ,  R. Michael Van Dam ,  James R. Heath

IPC: G01N33/00

CPC classification number: B01F11/0042 ,  B01F11/0071 ,  B01F13/0059 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00396 ,  B01J2219/00398 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00731 ,  B01J2219/00759 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00858 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00907 ,  B01J2219/00916 ,  B01J2219/00927 ,  B01J2219/00986

Abstract: Described herein are automated, integrated microfluidic device comprising a chemical reaction chip comprising for performing chemical reaction, a microscale column integrated with the chip and configured for liquid flow from the column to at least one flow channel, and wherein the fluid flow into the column is controlled by on-chip valves; and comprising at least two on-chip valves for controlling fluid flow in the microfluidic device.

Abstract translation: 本文描述的是包括用于进行化学反应的化学反应芯片的自动化集成微流体装置，与芯片集成的微量柱，并且构造成用于从柱流到至少一个流动通道的液体流，并且其中流入塔的流体流是 由片上阀控制; 并且包括用于控制微流体装置中的流体流动的至少两个片上阀。

公开(公告)号：US08592214B2

公开(公告)日：2013-11-26

申请号：US13026086

申请日：2011-02-11

Applicant: David Louis Heiner ,  Steven P. Fambro ,  Theo Kotseroglou ,  Michal Lebl

Inventor： David Louis Heiner ,  Steven P. Fambro ,  Theo Kotseroglou ,  Michal Lebl

IPC: G01N21/01 ,  G01N21/75

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00421 ,  B01J2219/00423 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00698 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  Y10T436/115831

Abstract: Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using these systems.

Abstract translation: 本文公开了用于监测化学反应的系统。 该系统可以包括照明装置，用于获得化学反应混合物的图像的相机装置和用于处理从图像获得的数据的分析器程序。 还公开了使用这些系统监测化学反应进程的方法。

公开(公告)号：US20110256031A1

公开(公告)日：2011-10-20

申请号：US12932334

申请日：2011-02-22

Applicant: Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

Inventor： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00722

Abstract: Embodiments of the present invention are directed to automated-polymer-synthesis systems that include discrete reagent-solution-addition, wait-time, and reagent-solution-draining sub-systems which together significantly increase throughput and decrease sub-system idle time. The automated-polymer-synthesis systems that represent embodiments of the present invention additionally include switches at points in which carriers can be received from multiple input paths or output to multiple different output paths. The automated-polymer-synthesis systems that represent embodiments of the present invention generally include an input spur and output spur in addition to a main loop, allowing carriers containing only completed polymers to be removed and new carriers input, so that carriers traverse the automated-polymer-synthesis systems independently from one another.

Abstract translation: 本发明的实施方案涉及自动聚合物合成系统，其包括离散的试剂 - 溶液添加，等待时间和试剂 - 溶液 - 排出子系统，这些子系统共同显着增加生产量并减少子系统空闲时间。 代表本发明实施例的自动化 - 聚合物合成系统另外包括在多个输入路径上可以接收载波或输出到多个不同输出路径的点处的交换机。 代表本发明实施例的自动化 - 聚合物 - 合成系统通常包括除了主回路之外的输入支线和输出支线，允许仅去除已完成的聚合物的载流子和新的载流子输入，使得载流子穿过自动化 - 聚合物合成系统彼此独立。

公开(公告)号：US20110236270A1

公开(公告)日：2011-09-29

申请号：US12932337

申请日：2011-02-22

Applicant: Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

Inventor： Hsing-Yeh Parker ,  John C. Tabone ,  John Mulligan

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00722

Abstract: Embodiments of the present invention include processing steps and subsystems, within automated-biopolymer-synthesis systems and within other automated systems for organic-chemistry-based processing, for removing reagent solutions and solvents from reaction chambers following various synthetic reaction steps and washing steps undertaken during biopolymer synthesis. Embodiments of the present invention employ any of various different types of liquid-absorbing materials to wick, or remove by capillary action, liquids from reaction chambers. Wicking-based methods and subcomponents of the present invention remove significantly greater fractions of solutions from reaction chambers than conventional methods and subsystems and, in addition, are mechanically simpler and produce fewer deleterious side effects than currently used methods and subsystems.

Abstract translation: 本发明的实施方案包括在自动生物聚合物 - 合成系统内和在用于基于有机化学的处理的其他自动化系统内的处理步骤和子系统，用于在各种合成反应步骤之后从反应室中除去试剂溶液和溶剂，并在 生物聚合物合成。 本发明的实施例使用各种不同类型的液体吸收材料中的任何一种来吸收或者通过毛细管作用从反应室中除去液体。 与常规方法和子系统相比，本发明的基于芯吸的方法和子部件从常规方法和子系统中除去反应室的溶液的显着更多的部分，此外，与目前使用的方法和子系统相比，机械上更简单并且产生较少有害的副作用。

公开(公告)号：US07887752B2

公开(公告)日：2011-02-15

申请号：US10762931

申请日：2004-01-21

Applicant: David Louis Heiner ,  Steven P. Fambro ,  Theo Kotseroglou ,  Michal Lebl

Inventor： David Louis Heiner ,  Steven P. Fambro ,  Theo Kotseroglou ,  Michal Lebl

IPC: G01N21/01 ,  G01N21/75

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/0036 ,  B01J2219/00414 ,  B01J2219/00421 ,  B01J2219/00423 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00698 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  Y10T436/115831

Abstract: Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using the these systems.

Abstract translation: 本文公开了用于监测化学反应的系统。 该系统可以包括照明装置，用于获得化学反应混合物的图像的相机装置和用于处理从图像获得的数据的分析器程序。 还公开了使用这些系统监测化学反应进程的方法。

公开(公告)号：US07488451B2

公开(公告)日：2009-02-10

申请号：US10901942

申请日：2004-07-28

Applicant: Michael A. Zarowitz ,  Glenn R. Edwards

Inventor： Michael A. Zarowitz ,  Glenn R. Edwards

IPC: G01N35/10

CPC classification number: G01N35/1011 ,  B01J2219/00364 ,  B01J2219/00414 ,  B01J2219/00423 ,  B01J2219/00466 ,  B01J2219/005 ,  B01L3/021 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/168 ,  B01L2400/0487 ,  C12M33/04 ,  G01N35/026

Abstract: Systems, including methods and apparatus, for particle manipulation to perform assays.

Abstract translation: 用于进行测定的粒子操作的系统，包括方法和装置。

公开(公告)号：US07364848B2

公开(公告)日：2008-04-29

申请号：US10526261

申请日：2003-09-01

Applicant: Marinus Gerardus Johannes Van Beuningen ,  Tim Kievits

Inventor： Marinus Gerardus Johannes Van Beuningen ,  Tim Kievits

IPC: C12Q1/68 ,  C12N15/11 ,  C12N9/12 ,  C12N9/14

CPC classification number: B01L3/5023 ,  B01J2219/00414 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00545 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00664 ,  B01J2219/00722 ,  B01J2219/00729 ,  B01L3/502707 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0825 ,  B01L2400/0487 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q2531/143 ,  C12Q2565/1015 ,  C12Q2565/631 ,  C12Q2565/629 ,  C12Q2565/101

Abstract: The present invention relates to methods for identifying analyte nucleic acids present in a sample comprising nucleic acid molecules, said method comprising the steps of: (a) providing a porous substrate, said substrate comprising a plurality of micro-channels, wherein said micro-channels have immobilized thereon a target molecule capable of binding to an analyte present in said sample; wherein said channels are further provided with analyte amplification components, a reporter system; and said sample; (b) amplifying analyte nucleic acid molecules present within said sample; and (c) allowing binding to take place between an amplified analyte nucleic acid obtained in step (b), said target molecule immobilized onto the channels of said substrate and said reporter system, wherein said reporter system allows detecting whether binding has occurred between said target molecule and said analyte nucleic acid. The present invention further relates to the use of said methods as well as kits for carrying out said methods.

Abstract translation: 本发明涉及用于鉴定存在于包含核酸分子的样品中的分析物核酸的方法，所述方法包括以下步骤：（a）提供多孔基底，所述基底包括多个微通道，其中所述微通道 在其上固定有能够结合存在于所述样品中的分析物的靶分子; 其中所述通道还具有分析物扩增组分，报道系统; 并说样品; （b）扩增存在于所述样品中的分析物核酸分子; 和（c）允许在步骤（b）中获得的扩增的分析物核酸，固定在所述底物的通道上的所述靶分子和所述报道系统之间进行结合，其中所述报道系统允许检测是否在所述靶 分子和所述分析物核酸。 本发明还涉及所述方法的使用以及用于实施所述方法的试剂盒。