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公开(公告)号：US20210149175A1
公开(公告)日：2021-05-20
申请号：US17082320
申请日：2020-10-28
发明人： Kai RITSCHEL , Marco BINGEL , Patric PELZER
摘要： An optical imaging device for a microscope comprises a first optical system configured to form a first optical image corresponding to a first region of a sample in accordance with a first imaging mode, a second optical system configured to form a second optical image corresponding to a second region of said sample, wherein said first and second regions spatially coincide in a target region of said sample and said first and second imaging modes are different from each other, a memory storing first distortion correction data suitable for correcting a first optical distortion caused by said first optical system in said first optical image, second distortion correction data suitable for correcting a second optical distortion caused by said second optical system in said second optical image, and transformation data suitable for correcting positional misalignment between said first and second optical images, and a processor which is configured to process first image data representing said first optical image based on said first distortion correction data for generating first distortion corrected image data, to process second image data representing said second optical image based on said second distortion correction data for generating second distortion corrected image data; and to combine said first and second distortion corrected image data based on said transformation data for generating combined image data representing a combined image which corresponds to said target region of said object.
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公开(公告)号：US20210063714A1
公开(公告)日：2021-03-04
申请号：US16977820
申请日：2019-02-20
发明人： Florian FAHRBACH , Werner KNEBEL , Peter POTT , Wernher FOUQUET
摘要： An optical device for illuminating a sample located in a sample volume with illumination light and for detecting scattered and/or fluorescent light from the sample includes an optical illumination assembly, an optical detection assembly and at least one attachment element. The optical illumination assembly is configured to transmit the illumination light along an illumination path into the sample volume. The optical detection assembly is configured to collect and relay the scattered and/or fluorescent light from the sample volume along a detection path. At least portions of the illumination path and/or of the detection path extend in the at least one attachment element.
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公开(公告)号：US10824116B2
公开(公告)日：2020-11-03
申请号：US16498393
申请日：2018-04-04
发明人： Bernd Widzgowski
摘要： A method forms a digital value from a clock signal and a digital data signal. The method includes sampling the clock signal in order to obtain a clock signal digital value sequence and sampling the digital data signal in order to obtain a data signal digital value sequence. Sampling points are ascertained from the clock signal digital value sequence, at which data signal digital values are extracted from the data signal digital value sequence. The digital value is formed from the data signal digital values.
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公开(公告)号：US10823951B2
公开(公告)日：2020-11-03
申请号：US16326937
申请日：2017-08-17
摘要： A method for imaging in a microscope with oblique illumination includes illuminating an object by an illumination beam path that is obliquely incident on an object plane of the microscope. A microscopic image of the object and a corresponding digital image signal are produced. The digital image signal is processed by digital image processing using a convolution kernel to increase contrast. An increased-contrast digital image is produced from the processed digital image signal.
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公开(公告)号：US10705356B2
公开(公告)日：2020-07-07
申请号：US15611802
申请日：2017-06-02
摘要： A method for adjusting an intensity of a light beam in an optical arrangement includes passing the light beam through an acousto-optical tunable filter (AOTF). The intensity of the light beam is adjusted as a function of frequency and/or amplitude of a sound wave with which the AOTF is operated. The amplitude of the sound wave at a specified sound wave frequency is selected such that the amplitude is larger than would be required to achieve a first maximum diffraction efficiency for a specified wavelength or for a specified wavelength spectrum of the light beam. The amplitude of the sound wave is also selected such that a value of an integral of a product of the transmission function of the AOTF and the wavelength spectrum of the light beam is larger than at a value of the amplitude to be selected to achieve the first maximum.
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公开(公告)号：US10698192B2
公开(公告)日：2020-06-30
申请号：US16352875
申请日：2019-03-14
发明人： Frank Sieckmann , Urban Liebel
摘要： A microscopy system provides for scanning a sample by a microscope, which is at least in part electrically or electronically controllable and in which a plurality of images or digital images are generatable in a scanning operation at different times and/or different locations of the sample. The microscopy system includes a control computer configured to control the microscope, as well as further computers that are integratable into the microscopy system such that an adaptation of the microscopy system is possible dynamically and/or during the scanning operation using at least one of the further computers.
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公开(公告)号：US10481374B2
公开(公告)日：2019-11-19
申请号：US15426118
申请日：2017-02-07
发明人： Frank Sieckmann , Urban Liebel
摘要： A method for scanning a sample using an electrically or electronically controllable microscope includes performing a continuous scanning of the sample so as to repeatedly generate a plurality of images of the sample, each of the plurality of images corresponding to a different time, the microscope being controlled via a control computer during the scanning. The plurality of images are analyzed using at least one second computer connected via a network, wherein the at least one second computer is configured to classify each of the plurality of images as one of interesting and non-interesting while the continuous scanning of the sample with the microscope continues. The continuous scanning of the sample is automatically influenced based on the classifying of the images performed by the at least one second computer.
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公开(公告)号：US10429630B2
公开(公告)日：2019-10-01
申请号：US15771341
申请日：2016-10-28
摘要： A fluorescence microscope has an incident fluorescence illumination unit generating an incident fluorescence illumination beam path a multi-band fluorescence filter system encompassing a multi-band beam splitter for deflecting the incident fluorescence illumination beam path into an objective of the microscope and onto a specimen. The filter system has a multi-band blocking filter that at least partly transmits a fluorescence emission beam path proceeding from the specimen; and having a digital camera for generating a fluorescence image of the specimen. The microscope has a bright-field transmitted illumination unit for generating a bright-field transmitted illumination beam path for transmitted illumination of the specimen. The filter system is arranged fixedly so that upon acquisition of a bright-field image of the specimen, the system remains in a bright-field transmitted light beam path proceeding from the specimen. The digital camera has a white balance function that is executed before the bright-field image is generated.
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公开(公告)号：US20190278072A1
公开(公告)日：2019-09-12
申请号：US16310472
申请日：2017-06-21
发明人： Florian FAHRBACH , Lars FRIEDRICH , Werner KNEBEL
摘要： An illumination apparatus for a microscope, for producing a de-excitation or switching light distribution, includes a light source configured to produce a primary illumination light beam and a beam splitter configured to divide the primary illumination light beam into two partial illumination light beams. An illumination objective is configured to focus the partial illumination light beams onto and/or into a sample such that the partial illumination light beams extend, spatially separated from one another, through an entry pupil of the illumination objective and are spatially superposed on and/or in the sample after passing through the illumination objective. A phase influencer is configured to cause a relative phase offset of the partial illumination light beams with respect to one another in such a way that the partial illumination light beams in the entry pupil of the illumination objective have a phase offset of π.
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