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    LUMINESCENT LIVE AND DEAD CELL ASSAY
    53.
    发明申请
    LUMINESCENT LIVE AND DEAD CELL ASSAY 有权
    LUMINESCENT LIVE和死亡细胞测定

    公开(公告)号：US20120149045A1

    公开(公告)日：2012-06-14

    申请号：US12100889

    申请日：2008-04-10

    申请人： Andrew Niles

    发明人： Andrew Niles

    IPC分类号： C12Q1/66 ,  C12Q1/37

    CPC分类号： C12Q1/66 ,  C12Q1/37

    摘要： A method to detect live and dead cells in a sample with one bioluminogenic reagent is provided.

    摘要翻译： 提供了用一种生物发光试剂检测样品中活细胞和死细胞的方法。

    RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags
    54.
    发明授权
    RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags 有权
    RF销售点和交付方式以及与远程计算机通信的系统，具有读取大量RF标签的功能

    公开(公告)号：US08113425B2

    公开(公告)日：2012-02-14

    申请号：US13086575

    申请日：2011-04-14

    申请人： Michael Dearing ,  Gediminas Vidugiris ,  William A. Linton ,  John Linton ,  Julia E. Krueger

    发明人： Michael Dearing ,  Gediminas Vidugiris ,  William A. Linton ,  John Linton ,  Julia E. Krueger

    IPC分类号： G06K17/60

    CPC分类号： G06Q10/08 ,  F25D2500/06 ,  F25D2700/08 ,  G06F19/00 ,  G06K7/10336 ,  G06K2017/0051 ,  G06K2017/0067 ,  G06Q10/087 ,  G06Q30/06 ,  G06Q30/0603 ,  G07F9/026 ,  G07G1/0045

    摘要： A method and system for providing point-of-sale and point-of-delivery and/or distribution of products in a restricted access unit near the customer. The method and system utilize products equipped with radio frequency tags and reduce the effects of energy sharing, shadowing, and nulls. In one embodiment, a plurality of RF tagged products are placed within a refrigerator, cabinet, or other micro-warehouse that has a door or opening that can detect access to the micro-warehouse. In one embodiment, one or more antennas are positioned within the door. Each antenna may have a transmission line of sight and be configured to emit a signal at predefined frequencies. Each antenna generates an electromagnetic field within the micro-warehouse. In one embodiment, the products are positioned in one or more bins, compartments, or similar devices located within the micro-warehouse such that at least two of the plurality of products are spaced a distance from each other to reduce energy sharing. The electromagnetic field is moved or altered within the micro-warehouse through the use of reflectors, devices that move the antennas, or other mechanisms.

    摘要翻译： 一种用于在客户附近的受限访问单元中提供销售点和交货点和/或分发产品的方法和系统。 该方法和系统利用配备射频标签的产品，减少能量共享，阴影和零点的影响。 在一个实施例中，将多个RF标签的产品放置在具有门或开口的冰箱，橱柜或其他微型仓库内，该门或开口可以检测对微型仓库的通道。 在一个实施例中，一个或多个天线位于门内。 每个天线可以具有传输线，并被配置为以预定的频率发射信号。 每个天线在微型仓库内产生一个电磁场。 在一个实施例中，将产品定位在位于微仓库内的一个或多个仓，隔间或类似装置中，使得多个产品中的至少两个彼此间隔开一定距离以减少能量共享。 通过使用反射器，移动天线的装置或其他机构，在微型仓库内移动或改变电磁场。

    Luminescence-based methods and probes for measuring cytochrome P450 activity
    55.
    发明授权
    Luminescence-based methods and probes for measuring cytochrome P450 activity 有权
    用于测量细胞色素P450活性的基于发光的方法和探针

    公开(公告)号：US08106052B2

    公开(公告)日：2012-01-31

    申请号：US12754164

    申请日：2010-04-05

    申请人： James J. Cali ,  Dieter Klaubert ,  William Daily ,  Samuel Kin Sang Ho ,  Susan Frackman ,  Erika Hawkins ,  Keith V. Wood

    发明人： James J. Cali ,  Dieter Klaubert ,  William Daily ,  Samuel Kin Sang Ho ,  Susan Frackman ,  Erika Hawkins ,  Keith V. Wood

    IPC分类号： A61K31/403 ,  A61K31/4164 ,  A61K31/422 ,  A61K31/437 ,  A61K31/4985 ,  C07D413/04 ,  C07D417/04 ,  C07D471/04 ,  C07D487/04

    CPC分类号： G01N33/502 ,  A61K49/0008 ,  C07D417/04 ,  C12Q1/66 ,  G01N33/5088 ,  G01N33/582 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2500/10

    摘要： The present invention provides methods, compositions, substrates, and kits useful for analyzing the metabolic activity in cells, tissue, and animals and for screening test compounds for their effect on cytochrome P450 activity. In particular, a one-step and two-step methods using luminogenic molecules, e.g. luciferin or coelenterazines, that are cytochrome P450 substrates and that are also bioluminescent enzyme, e.g., luciferase, pro-substrates are provided. Upon addition of the luciferin derivative or other luminogenic molecule into a P450 reaction, the P450 enzyme metabolizes the molecule into a bioluminescent enzyme substrate, e.g., luciferin and/or luciferin derivative metabolite, in a P450 reaction. The resulting metabolite(s) serves as a substrate of the bioluminescent enzyme, e.g., luciferase, in a second light-generating reaction. Luminescent cytochrome P450 assays with low background signals and high sensitivity are disclosed and isoform selectivity is demonstrated. The present invention also provides an improved method for performing luciferase reactions which employs added pyrophosphatase to remove inorganic pyrophosphate, a luciferase inhibitor which may be present in the reaction mixture as a contaminant or may be generated during the reaction. The present method further provides a method for stabilizing and prolonging the luminescent signal in a luciferase-based assay using luciferase stabilizing agents such as reversible luciferase inhibitors.

    摘要翻译： 本发明提供了可用于分析细胞，组织和动物中的代谢活性以及筛选测试化合物对细胞色素P450活性的影响的方法，组合物，底物和试剂盒。 特别地，使用发光分子的一步和两步法。 荧光素或coelenterazines，其是细胞色素P450底物并且也是生物发光酶，例如萤光素酶，原底物。 在P450反应中加入荧光素衍生物或其他发光分子时，P450酶在P450反应中将分子代谢为生物发光酶底物，例如荧光素和/或荧光素衍生物代谢产物。 所产生的代谢物在第二次发光反应中用作生物发光酶，例如荧光素酶的底物。 公开了具有低背景信号和高灵敏度的发光细胞色素P450测定，证明了同工型选择性。 本发明还提供一种用于进行荧光素酶反应的改进方法，其使用添加的焦磷酸酶来除去无机焦磷酸盐，荧光素酶抑制剂，其可作为污染物存在于反应混合物中，或可能在反应期间产生。 本发明方法还提供了一种使用荧光素酶稳定剂如可逆荧光素酶抑制剂在基于荧光素酶的测定中稳定和延长发光信号的方法。

    RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags
    58.
    发明授权
    RF point of sale and delivery method and system using communication with remote computer and having features to read a large number of RF tags 有权
    RF销售和交付方式以及与远程计算机通信的系统，具有阅读大量RF标签的功能

    公开(公告)号：US07938326B2

    公开(公告)日：2011-05-10

    申请号：US12694361

    申请日：2010-01-27

    申请人： Michael Dearing ,  Gediminas Vidugiris ,  William A. Linton ,  John Linton ,  Julia E. Krueger

    发明人： Michael Dearing ,  Gediminas Vidugiris ,  William A. Linton ,  John Linton ,  Julia E. Krueger

    IPC分类号： G06F17/00

    CPC分类号： G06Q10/08 ,  F25D2500/06 ,  F25D2700/08 ,  G06F19/00 ,  G06K7/10336 ,  G06K2017/0051 ,  G06K2017/0067 ,  G06Q10/087 ,  G06Q30/06 ,  G06Q30/0603 ,  G07F9/026 ,  G07G1/0045

    摘要： A method and system for providing point-of-sale and point-of-delivery and/or distribution of products in a restricted access unit near the customer. The method and system utilize products equipped with radio frequency tags and reduce the effects of energy sharing, shadowing, and nulls. In one embodiment, a plurality of RF tagged products are placed within a refrigerator, cabinet, or other micro-warehouse that has a door or opening that can detect access to the micro-warehouse. In one embodiment, one or more antennas are positioned within the door. Each antenna may have a transmission line of sight and be configured to emit a signal at predefined frequencies. Each antenna generates an electromagnetic field within the micro-warehouse. In one embodiment, the products are positioned in one or more bins, compartments, or similar devices located within the micro-warehouse such that at least two of the plurality of products are spaced a distance from each other to reduce energy sharing. The electromagnetic field is moved or altered within the micro-warehouse through the use of reflectors, devices that move the antennas, or other mechanisms.

    摘要翻译： 一种用于在客户附近的受限访问单元中提供销售点和交货点和/或分发产品的方法和系统。 该方法和系统利用配备射频标签的产品，减少能量共享，阴影和零点的影响。 在一个实施例中，将多个RF标签的产品放置在具有门或开口的冰箱，橱柜或其他微型仓库内，该门或开口可以检测对微型仓库的通道。 在一个实施例中，一个或多个天线位于门内。 每个天线可以具有传输线，并被配置为以预定的频率发射信号。 每个天线在微型仓库内产生一个电磁场。 在一个实施例中，将产品定位在位于微仓库内的一个或多个仓，隔间或类似装置中，使得多个产品中的至少两个彼此间隔开一定距离以减少能量共享。 通过使用反射器，移动天线的装置或其他机构，在微型仓库内移动或改变电磁场。

    METHODS AND KITS FOR ISOLATING CELLS
    60.
    发明申请
    METHODS AND KITS FOR ISOLATING CELLS 审中-公开
    分离细胞的方法和试剂盒

    公开(公告)号：US20100143878A1

    公开(公告)日：2010-06-10

    申请号：US12444075

    申请日：2007-10-08

    申请人： Ryan J. Olson ,  Rex M. Bitner ,  Allan M. Tereba ,  Laura L. Bozek

    发明人： Ryan J. Olson ,  Rex M. Bitner ,  Allan M. Tereba ,  Laura L. Bozek

    IPC分类号： A01N1/02 ,  C12N1/08 ,  C12N1/00

    CPC分类号： C12N5/0612 ,  C12N1/06 ,  C12N5/061 ,  C12N15/1013

    摘要： Disclosed are methods for differential extraction of a target component from a sample which is predominantly composed of other types of non-target cells that can be lysed using methods that do not lyse the target cells, so that the target material can be purified away from the lysed non-target material. One exemplary method is directed to isolating sperm cells from an aqueous sample and kits for performing same.

    摘要翻译： 公开了从主要由其他类型的非靶细胞组成的样品中差异提取靶组分的方法，所述其他类型的非靶细胞可以使用不裂解靶细胞的方法裂解，从而可以将目标物质从 裂解的非目标材料。 一个示例性的方法是将精子细胞与含水样品分离，并进行相同的试剂盒。