公开(公告)号：US20100143878A1

公开(公告)日：2010-06-10

申请号：US12444075

申请日：2007-10-08

申请人： Ryan J. Olson ,  Rex M. Bitner ,  Allan M. Tereba ,  Laura L. Bozek

发明人： Ryan J. Olson ,  Rex M. Bitner ,  Allan M. Tereba ,  Laura L. Bozek

IPC分类号： A01N1/02 ,  C12N1/08 ,  C12N1/00

CPC分类号： C12N5/0612 ,  C12N1/06 ,  C12N5/061 ,  C12N15/1013

摘要： Disclosed are methods for differential extraction of a target component from a sample which is predominantly composed of other types of non-target cells that can be lysed using methods that do not lyse the target cells, so that the target material can be purified away from the lysed non-target material. One exemplary method is directed to isolating sperm cells from an aqueous sample and kits for performing same.

摘要翻译： 公开了从主要由其他类型的非靶细胞组成的样品中差异提取靶组分的方法，所述其他类型的非靶细胞可以使用不裂解靶细胞的方法裂解，从而可以将目标物质从 裂解的非目标材料。 一个示例性的方法是将精子细胞与含水样品分离，并进行相同的试剂盒。

公开(公告)号：US06673631B1

公开(公告)日：2004-01-06

申请号：US09377986

申请日：1999-08-20

申请人： Allan M. Tereba ,  Rex M. Bitner ,  Susan C. Koller ,  Craig E. Smith ,  Daniel D. Kephart ,  Steven J. Ekenberg

发明人： Allan M. Tereba ,  Rex M. Bitner ,  Susan C. Koller ,  Craig E. Smith ,  Daniel D. Kephart ,  Steven J. Ekenberg

IPC分类号： C12Q168

CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  Y10S977/838 ,  Y10S977/904 ,  Y10S977/916 ,  Y10S977/92 ,  Y10S977/924 ,  Y10S977/96

摘要： The present invention provides methods for isolating a defined quantity of DNA target material from other substances in a medium. The method may be carried out using a known quantity of a silica-containing solid support, such as silica magnetic particles, having a definable capacity for reversibly binding DNA target material, and DNA target material in excess of the binding capacity of the particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the DNA target material in a mixture of the medium and particles, and separating the complex from the mixture using external magnetic force. The DNA target material may then be eluted from the complex. The quantity of DNA target material eluted may be determined based on a calibration model. The methods of the present invention permit isolation of DNA target material which is within a known quantity range. The methods of the invention eliminate the step of quantitating purified biological samples prior to further processing, such as amplification, Short Tandem Repeat (STR) analysis, and DNA sequencing. Samples of the DNA target materials may be obtained from liquid or solid media, such as liquid blood or paper.

摘要翻译： 本发明提供了将定义量的DNA靶物质与介质中的其它物质分离的方法。 该方法可以使用已知量的具有可定义的可逆结合DNA靶材料的能力的二氧化硅固体载体，例如二氧化硅磁性颗粒和超过颗粒结合能力的DNA靶材料进行。 本发明的方法包括在介质和颗粒的混合物中形成二氧化硅磁性颗粒和DNA靶材料的复合物，并且使用外部磁力将复合物与混合物分离。 然后DNA靶物质可以从络合物中洗脱出来。 可以基于校准模型确定洗脱的DNA靶物质的量。 本发明的方法允许分离已知量范围内的DNA靶材料。 本发明的方法消除了在进一步加工之前定量纯化的生物样品的步骤，例如扩增，短串联重复（STR）分析和DNA测序。 可以从液体或固体培养基（例如液体血液或纸）获得DNA靶材料的样品。