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    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS
    51.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS 有权
    用于生产对苯二酸和其他化合物的微生物

    公开(公告)号：US20100330626A1

    公开(公告)日：2010-12-30

    申请号：US12875084

    申请日：2010-09-02

    申请人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    发明人： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

    IPC分类号： C12P17/10 ,  C12N1/00 ,  C12P7/46

    CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

    摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

    摘要翻译： 本发明提供了具有己二酸，6-氨基己酸或己内酰胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应己二酸，6-氨基己酸或己内酰胺途径中的酶的外源核酸。 本发明另外提供了生产己二酸，6-氨基己酸或己内酰胺的方法。 该方法可以包括培养己二酸，6-氨基己酸或己内酰胺生产微生物，其中微生物生物体表达至少一种编码己二酸，6-氨基己酸或己内酰胺途径酶的外源核酸，其量足以产生相应的产物 在条件下和足够的时间内生产己二酸，6-氨基己酸或己内酰胺。

    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    53.
    发明授权
    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate 有权
    富马酸盐，苹果酸盐和丙烯酸酯的生物合成的微生物和方法

    公开(公告)号：US08129154B2

    公开(公告)日：2012-03-06

    申请号：US12486724

    申请日：2009-06-17

    申请人： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    发明人： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    IPC分类号： C12P7/46

    CPC分类号： C12P7/46 ,  C12N9/88 ,  C12P7/40

    摘要： A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    摘要翻译： 非天然存在的真核生物或原核生物包括当基因破坏降低酶的活性时，编码在赋予生物体中增加的富马酸盐，苹果酸或丙烯酸酯产生的酶的基因中发生的一种或多种基因破坏。 一个或多个基因破坏使丙烯酸酯的生产增加到生物体上。 生产丙烯酸酯的生物体具有丙烯酸酯途径，至少一种编码丙烯酸酯途径酶的外源核酸以足够的量表达以产生丙烯酸酯，丙烯酸酯途径包含脱羧酶。 产生富马酸盐，苹果酸盐或丙烯酸盐的方法包括培养这些生物体。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE
    55.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE 审中-公开
    富马酸和丙烯酸的生物合成的微生物和方法

    公开(公告)号：US20120237990A1

    公开(公告)日：2012-09-20

    申请号：US13372332

    申请日：2012-02-13

    申请人： Mark J. BURK ,  Anthony P. Burgard ,  Priti Pharkya

    发明人： Mark J. BURK ,  Anthony P. Burgard ,  Priti Pharkya

    IPC分类号： C12P7/40 ,  C12N1/15 ,  C12N1/19

    CPC分类号： C12P7/46 ,  C12N9/88 ,  C12P7/40

    摘要： A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    摘要翻译： 非天然存在的真核生物或原核生物包括当基因破坏降低酶的活性时，编码在赋予生物体中增加的富马酸盐，苹果酸或丙烯酸酯产生的酶的基因中发生的一种或多种基因破坏。 一个或多个基因破坏使丙烯酸酯的生产增加到生物体上。 生产丙烯酸酯的生物体具有丙烯酸酯途径，至少一种编码丙烯酸酯途径酶的外源核酸以足够的量表达以产生丙烯酸酯，丙烯酸酯途径包含脱羧酶。 产生富马酸盐，苹果酸盐或丙烯酸盐的方法包括培养这些生物体。

    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    57.
    发明授权
    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate 有权
    富马酸盐，苹果酸盐和丙烯酸酯的生物合成的微生物和方法

    公开(公告)号：US09062330B2

    公开(公告)日：2015-06-23

    申请号：US13372332

    申请日：2012-02-13

    申请人： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    发明人： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    IPC分类号： C12P7/40 ,  C12P7/46

    CPC分类号： C12P7/46 ,  C12N9/88 ,  C12P7/40

    摘要： A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    摘要翻译： 非天然存在的真核生物或原核生物包括当基因破坏降低酶的活性时，编码在赋予生物体中增加的富马酸盐，苹果酸或丙烯酸酯产生的酶的基因中发生的一种或多种基因破坏。 一个或多个基因破坏使丙烯酸酯的生产增加到生物体上。 生产丙烯酸酯的生物体具有丙烯酸酯途径，至少一种编码丙烯酸酯途径酶的外源核酸以足够的量表达以产生丙烯酸酯，丙烯酸酯途径包含脱羧酶。 产生富马酸盐，苹果酸盐或丙烯酸盐的方法包括培养这些生物体。

    Method for redesign of microbial production systems
    59.
    发明授权
    Method for redesign of microbial production systems 有权
    微生物生产系统重新设计方法

    公开(公告)号：US07826975B2

    公开(公告)日：2010-11-02

    申请号：US10929091

    申请日：2004-08-26

    申请人： Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    发明人： Costas D. Maranas ,  Anthony P. Burgard ,  Priti Pharkya

    IPC分类号： G06F19/00

    CPC分类号： G01N33/5005 ,  G01N33/54366 ,  G06F19/12 ,  G06F19/18

    摘要： A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

    摘要翻译： 用于鉴定添加到生物体特异性代谢网络以实现宿主期望生物转化的功能的计算机辅助方法包括从通用数据库获得反应以提供化学计量平衡，至少部分地基于化学计量平衡路径， 反应和底物以使生产宿主中的非天然功能数量最小化，并将至少一种化学计量平衡的途径并入宿主以提供所需的生物转化。 可以存储经修改的代谢网络的表示。

    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL
    60.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL 审中-公开
    用于生产甲基乙基酮和2-丁醇的微生物

    公开(公告)号：US20100184173A1

    公开(公告)日：2010-07-22

    申请号：US12618545

    申请日：2009-11-13

    申请人： Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    发明人： Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    IPC分类号： C12P7/26 ,  C12N1/00 ,  C12P7/16

    CPC分类号： C12P7/16 ,  C12N15/52 ,  C12P7/26 ,  Y02E50/10

    摘要： A non-naturally occurring microbial organism having a methyl ethyl ketone pathway includes at least one exogenous nucleic acid encoding a methyl ethyl ketone pathway enzyme expressed in a sufficient amount to produce methyl ethyl ketone. The methyl ethyl ketone pathway includes a β-ketothiolase, a β-ketovalerate decarboxylase and an enzyme selected from the group consisting of a β-ketovaleryl-CoA hydrolase and a β-ketovaleryl-CoA transferase. Alternatively, the methyl ethyl ketone pathway includes a 2-methylacetoacetyl-CoA thiolase, a 2-methylacetoacetate decarboxylase and an enzyme selected from the group consisting of a 2-methylacetoacetyl-CoA hydrolase and a 2-methylacetoacetyl-CoA transferase. Either pathway can further include a methyl ethyl ketone reductase to produce 2-BuOH. A method for producing methyl ethyl ketone or 2-BuOH includes culturing these non-naturally occurring microbial organisms under conditions, and for a sufficient period of time, to produce methyl ethyl ketone or 2-BuOH.

    摘要翻译： 具有甲基乙基酮途径的非天然存在的微生物包括至少一种编码甲基乙基酮途径酶的外源核酸，其以足以产生甲基乙基酮的量表达。 甲基乙基酮途径包括α-酮硫解酶，α-酮戊二酸脱羧酶和选自α-酮戊酰基-CoA水解酶和一种α-酮戊酰基-CoA转移酶的酶。 或者，甲基乙基酮途径包括2-甲基乙酰乙酰辅酶A硫解酶，2-甲基乙酰乙酸脱羧酶和选自2-甲基乙酰乙酰辅酶A水解酶和2-甲基乙酰乙酰辅酶A转移酶的酶。 任一途径还可以包括甲基乙基酮还原酶以产生2-BuOH。 一种生产甲基乙基酮或2-BuOH的方法包括在条件和足够的时间内培养这些非天然存在的微生物生产甲基乙基酮或2-BuOH。