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51.发明授权Illumination apparatus for microscope and image processing apparatus using the same 有权用于显微镜的照明装置和使用其的图像处理装置公开(公告)号：US07126752B2
公开(公告)日：2006-10-24
申请号：US10718884
申请日：2003-11-20
申请人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
发明人： Atsushi Miyawaki , Takashi Fukano , Yasushi Aono
CPC分类号： G02B21/06 , G02B21/16 , G02B21/365
摘要： An illumination apparatus for a microscope and an image processing apparatus using the illumination apparatus include a light source, a semi-transmissive mirror splitting a light beam from the light source into two beams of the first and second irradiation light, two excitation filters selecting the wavelengths of the first and second irradiation light, a semi-transmissive mirror synthesizing individual beams of the first and second irradiation light whose wavelengths are selected, into a single beam, a dichroic mirror directing a light beam synthesized by the semi-transmissive mirror toward a specimen and transmitting light from the specimen, an objective lens, cameras imaging fluorescent light from the specimen after being separated into fluorescent light excited by the first and second wavelengths, and an image processing section processing fluorescent images formed by imaging elements.
摘要翻译： 用于显微镜的照明装置和使用该照明装置的图像处理装置包括光源，将来自光源的光束分成两束第一和第二照射光的半透射镜，两个激发滤光器选择波长 第一和第二照射光的第一和第二照射光的半透射镜将将波长选择的第一和第二照射光的各个光束合成为单个光束，将由半透射镜合成的光束引向样品的二向色镜 以及透射来自所述样本的物体，物镜，将被分离成由所述第一和第二波长激发的荧光的荧光从所述样本成像的摄像机，以及处理由成像元件形成的荧光图像的图像处理部。
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公开(公告)号：US07060869B2
公开(公告)日：2006-06-13
申请号：US09554000
申请日：2000-04-20
申请人： Roger Y. Tsien , Atsushi Miyawaki
发明人： Roger Y. Tsien , Atsushi Miyawaki
IPC分类号： A61K67/027
CPC分类号： C07K14/43595 , A01K2217/05 , C07K2319/00 , G01N33/542
摘要： Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.
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公开(公告)号：US10444124B2
公开(公告)日：2019-10-15
申请号：US14118150
申请日：2012-05-18
申请人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
发明人： Atsushi Miyawaki , Hiroshi Hama , Asako Sakaue
摘要： A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.
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公开(公告)号：US09989518B2
公开(公告)日：2018-06-05
申请号：US13389869
申请日：2010-10-08
IPC分类号： A61K31/7088 , G01N33/50 , G01N21/64 , G01N21/80
CPC分类号： G01N33/5005 , G01N21/6428 , G01N21/80 , G01N33/5035 , G01N2021/6419 , G01N2021/6421
摘要： This invention relates to a method for measuring autophagy in cells, comprising using, as a probe reagent, a single fluorescent protein, to measure a change in fluorescence properties of the fluorescent probe reagent depending on pH changes associated with autophagy, thereby determining the presence or activity of autophagy, wherein the single fluorescent protein is resistant to degrading enzyme activity in the lysosome or vacuole of the cell, it is not denatured or inactivated under acidic to neutral pH environment, and it is capable of changing excitation spectra or fluorescence spectra when located under the environments of acidic region and neutral region.
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公开(公告)号：US20120288883A1
公开(公告)日：2012-11-15
申请号：US13574400
申请日：2011-01-21
申请人： Atsushi Miyawaki , Masahiko Hirano
发明人： Atsushi Miyawaki , Masahiko Hirano
CPC分类号： C12Q1/37 , C07K2319/00 , C12N15/85
摘要： This invention relates to a probe reagent comprising, in order from the N-terminus to the C-terminus, the amino acid sequences of a fluorescent protein I, a peptide capable of terminating protein degradation (i.e., a degradation-terminating peptide), a spacer peptide, a fluorescent protein II, and a protein to be degraded, wherein the protein to be degraded is a protein degraded by the ubiquitin-proteasome system, and the probe reagent is degraded from the C-terminus, but that the degradation of the probe reagent is terminated at the degradation-terminating peptide, a nucleic acid encoding the probe reagent, and use of the probe reagent or the nucleic acid.
摘要翻译： 本发明涉及探针试剂，其从N末端到C末端的顺序包括荧光蛋白I的氨基酸序列，能够终止蛋白质降解的肽（即降解终止肽）， 间隔肽，荧光蛋白II和待降解的蛋白质，其中待降解的蛋白质是由泛素 - 蛋白酶体系统降解的蛋白质，并且探针试剂从C末端降解，但是 探针试剂终止于降解终止肽，编码探针试剂的核酸和探针试剂或核酸的使用。
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公开(公告)号：US08304240B2
公开(公告)日：2012-11-06
申请号：US12464038
申请日：2009-05-11
IPC分类号： C12N15/00
摘要： An object of the present invention is to provide a method for introducing a physiologically active substance such as a gene into a cell, which introduces a physiologically active substance such as any given gene into any given cell in a view under a microscope, while significantly reducing invasiveness to the cell, and a device used for the above method. The present invention provides a method for introducing a physiologically active substance into a cell, which comprises: allowing a physiologically active substance to attach around a needle having a diameter of 500 nm or less, provided that it is able to be inserted into a cell; and inserting the above-described needle into the cell; and a microinjection device for carrying out the aforementioned method.
摘要翻译： 本发明的目的是提供一种将生物活性物质如基因导入细胞的方法，该细胞在显微镜下以任何给定的基因引入任何给定基因的生理活性物质，同时显着降低 对细胞的侵袭性，以及用于上述方法的装置。 本发明提供了一种将生理活性物质引入细胞的方法，其包括：使生理活性物质附着在直径为500nm以下的针上，只要其能够插入细胞中即可。 并将上述针插入细胞中; 以及用于实施上述方法的显微注射装置。
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57.发明授权公开(公告)号：US07981658B2
公开(公告)日：2011-07-19
申请号：US12569464
申请日：2009-09-29
申请人： Atsushi Miyawaki , Takeharu Nagai
发明人： Atsushi Miyawaki , Takeharu Nagai
CPC分类号： C07K14/43595 , C07K2319/00
摘要： The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 46th phenylalanine residue is substituted with a leucine residue. According to the present invention, there are provided novel GFP or YEP mutants having an excellent maturation efficacy and having a decreased sensitivity to both of H+ and CI31.
摘要翻译： 本发明提供了具有绿色荧光蛋白的氨基酸序列，黄色荧光蛋白或其突变体的荧光蛋白，其中第46位苯丙氨酸残基被亮氨酸残基取代。 根据本发明，提供了具有优异成熟功效并且对H +和CI31都具有降低的敏感性的新型GFP或YEP突变体。
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公开(公告)号：US20100151514A1
公开(公告)日：2010-06-17
申请号：US12063498
申请日：2006-08-11
CPC分类号： C07K14/4727 , A23J1/04 , A23J3/04 , A23L33/17 , A61K38/00
摘要： Provided is a novel mucin-type glycoprotein and a method for producing the same. Specifically, a mucin-type glycoprotein having a repeat structure including 3 to 2000 repeating units each having an amino acid sequence represented by the formula I: Val-Xaa-Glu-Thr-Thr-Ala-Ala-Pro [wherein Xaa represents Val or Ile] (SEQ ID NO: 1), wherein one or more amino acid residues in the structure are bound to a sugar chain of one or more monosaccharides. Also provided is a composition containing the novel mucin-type glycoprotein. Further provided is a molecular weight marker containing the novel mucin-type glycoprotein.
摘要翻译： 本发明提供一种新型粘蛋白型糖蛋白及其制造方法。 具体地说，具有重复结构的粘蛋白型糖蛋白，其重复结构包括3〜2000个重复单元，每个重复单元各自具有由式I表示的氨基酸序列：Val-Xaa-Glu-Thr-Thr-Ala-Ala-Pro [其中Xaa表示Val或 Ile]（SEQ ID NO：1），其中结构中的一个或多个氨基酸残基与一个或多个单糖的糖链结合。 还提供含有新型粘蛋白型糖蛋白的组合物。 还提供含有新型粘蛋白型糖蛋白的分子量标记物。
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公开(公告)号：US20100100977A1
公开(公告)日：2010-04-22
申请号：US12450155
申请日：2008-02-06
申请人： Atsushi Miyawaki , Asako Sawano , Hisao Masai
发明人： Atsushi Miyawaki , Asako Sawano , Hisao Masai
CPC分类号： G01N33/50 , A01K67/0275 , A01K2207/12 , A01K2217/05 , A01K2227/105 , A01K2267/0331 , A01K2267/0393 , A61K49/0008 , C07K14/4702 , C12N15/8509 , G01N33/5005
摘要： An object of an embodiment of the present invention is to provide a method with which it is possible to easily distinguish a proliferation phase of a cell cycle from a resting phase thereof in real time. The object of the embodiment of the present invention is attained by providing a method for performing phase identification of the cell cycle, the method including: visualizing one or more gene-expression products as markers whose amounts in a cell change in a cell-cycle dependent manner; and detecting the products so as to distinguish the proliferation phase of the cell cycle from the resting phase thereof.
摘要翻译： 本发明的一个实施方案的目的是提供一种可以实时地容易地将细胞周期的增殖期与其静止期区分开的方法。 本发明的实施方案的目的是通过提供一种用于进行细胞周期的相位鉴定的方法来实现，所述方法包括：将一种或多种基因表达产物可视化为其细胞中细胞周期依赖性变化的标记 方式; 并检测产物以区分细胞周期的增殖期与其静止期。
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公开(公告)号：US20090286319A1
公开(公告)日：2009-11-19
申请号：US12464038
申请日：2009-05-11
摘要： An object of the present invention is to provide a method for introducing a physiologically active substance such as a gene into a cell, which introduces a physiologically active substance such as any given gene into any given cell in a view under a microscope, while significantly reducing invasiveness to the cell, and a device used for the above method. The present invention provides a method for introducing a physiologically active substance into a cell, which comprises: allowing a physiologically active substance to attach around a needle having a diameter of 500 nm or less, provided that it is able to be inserted into a cell; and inserting the above-described needle into the cell; and a microinjection device for carrying out the aforementioned method.
摘要翻译： 本发明的目的是提供一种将生物活性物质如基因导入细胞的方法，该细胞在显微镜下以任何给定的基因引入任何给定基因的生理活性物质，同时显着降低 对细胞的侵袭性，以及用于上述方法的装置。 本发明提供了一种将生理活性物质引入细胞的方法，其包括：使生理活性物质附着在直径为500nm以下的针上，只要其能够插入细胞中即可。 并将上述针插入细胞中; 以及用于实施上述方法的显微注射装置。
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