公开(公告)号：US07981637B2

公开(公告)日：2011-07-19

申请号：US12976579

申请日：2010-12-22

申请人： Atsushi Miyawaki ,  Satoshi Karasawa

发明人： Atsushi Miyawaki ,  Satoshi Karasawa

IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04

CPC分类号： G01N33/542 ,  C07K14/37 ,  C07K2319/00 ,  G01N33/533 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2500/10

摘要： It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from Anthopleura inornata, which have certain property, and fluorescent proteins from Trachyphyllia geoffroyi and Scolymia vitiensis, which have certain fluorescent property.

摘要翻译： 本发明的目的是提供一种新型的生色蛋白和新的荧光蛋白。 本发明提供了具有一定性质的Anthopleura inornata衍生的色蛋白，以及具有一定荧光性质的来自Trachyphyllia geoffroyi和Scolymia vitiensis的荧光蛋白。

公开(公告)号：US07956172B2

公开(公告)日：2011-06-07

申请号：US10561041

申请日：2004-06-16

申请人： Atsushi Miyawaki ,  Hidekazu Tsutsui ,  Satoshi Karasawa

发明人： Atsushi Miyawaki ,  Hidekazu Tsutsui ,  Satoshi Karasawa

IPC分类号： C12N15/12

CPC分类号： C07K14/43504

摘要： It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from Montipora sp., Acropora sp. and Lobophytum crassum, and a novel chromoprotein derived from Actinia equine.

摘要翻译： 本发明的目的是提供一种新的荧光蛋白和新的色蛋白。 本发明提供了一种新型的从Montipora sp。，Acropora sp。 和Lobophytum crassum，以及衍生自猕猴桃的新型染色体蛋白。

公开(公告)号：US20100184116A1

公开(公告)日：2010-07-22

申请号：US12569464

申请日：2009-09-29

申请人： Atsushi MIYAWAKI ,  Takeharu NAGAI

发明人： Atsushi MIYAWAKI ,  Takeharu NAGAI

IPC分类号： C12Q1/02 ,  C07K14/28 ,  C07H21/04 ,  C12N5/00 ,  C12N15/63 ,  G01N21/76

CPC分类号： C07K14/43595 ,  C07K2319/00

摘要： The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 46th phenylalanine residue is substituted with a leucine residue. According to the present invention, there are provided novel GFP or YEP mutants having an excellent maturation efficacy and having a decreased sensitivity to both of H+ and Cr.

摘要翻译： 本发明提供了具有绿色荧光蛋白的氨基酸序列，黄色荧光蛋白或其突变体的荧光蛋白，其中第46位苯丙氨酸残基被亮氨酸残基取代。 根据本发明，提供了具有优异成熟功效并且对H +和Cr两者具有降低的敏感性的新型GFP或YEP突变体。

公开(公告)号：US07553624B2

公开(公告)日：2009-06-30

申请号：US10557906

申请日：2004-05-20

申请人： Takeharu Nagai ,  Atsushi Miyawaki

发明人： Takeharu Nagai ,  Atsushi Miyawaki

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/53 ,  C07H19/04 ,  C07H21/02

CPC分类号： G01N33/533

摘要： The present invention provides a method of analyzing the physiological function of a target substance by inactivating the physiological function of the target substance, comprising the steps of: (a) binding to the target substance a photoactive compound represented by formula (I): (in the formula, Q is a group for binding this compound with the target substance) to form a composite comprising the target substance and the photoactive compound; and (b) irradiating the obtained composite with light to inactivate the function of the target substance to which the photoactive compound has been bound, or to inactivate the function of the target substance at the site where the photoactive compound has been bound.

摘要翻译： 本发明提供了通过使目标物质的生理功能失活来分析目标物质的生理功能的方法，包括以下步骤：（a）与目标物质结合由式（I）表示的光活性化合物：（在 式中，Q为与化合物与目标物质结合的基团），形成包含目标物质和光活性化合物的复合体; 和（b）用光照射得到的复合物，使光活性化合物结合的目标物质的功能失活，或者使光活性化合物结合的位置失活目标物质的功能。

公开(公告)号：US07449151B2

公开(公告)日：2008-11-11

申请号：US10753569

申请日：2004-01-09

申请人： Masahiko Hirano ,  Masafumi Oshiro ,  Atsushi Miyawaki

发明人： Masahiko Hirano ,  Masafumi Oshiro ,  Atsushi Miyawaki

IPC分类号： G01N33/00

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6417 ,  G01N2021/6421

摘要： An analyzer for measuring an FRET (fluorescence resonance energy transfer) efficiency of a specimen containing a donor and an acceptor. The analyzer has an illuminator, an optical system, a detector and a calculator. The illuminator emits light for donor excitation and acceptor bleaching. The detector detects fluorescence from the specimen. The calculator calculates the FRET efficiency using the output of the detector. The detector independently detects the fluorescence in wavelength regions. One of the regions has a larger overlap with the fluorescence spectrum of the acceptor than with that of the donor.

摘要翻译： 用于测量含有供体和受体的样品的FRET（荧光共振能量转移）效率的分析仪。 分析仪具有照明器，光学系统，检测器和计算器。 照明器发光，用于供体激发和受主漂白。 检测器检测样品的荧光。 计算器使用检测器的输出计算FRET效率。 检测器独立地检测波长区域中的荧光。 其中一个区域与受体的荧光光谱重叠比与供体的荧光光谱重叠。

公开(公告)号：US20080166799A1

公开(公告)日：2008-07-10

申请号：US11739133

申请日：2007-04-24

申请人： Atsushi MIYAWAKI ,  Satoshi KARASAWA

发明人： Atsushi MIYAWAKI ,  Satoshi KARASAWA

IPC分类号： C12N5/06 ,  C07H21/04 ,  C12N15/63

CPC分类号： C07K14/37

摘要： An object of the present invention is to provide a novel fluorescent protein derived from organisms other than Aequorea victoria. According to the present invention, there is provided a fluorescent protein derived from Fungia sp., which has the following properties: (1) the excitation maximum wavelength is 455 nm, and the fluorescence maximum wavelength is 488 nm; (2) the molar absorption coefficient at 455 nm is 38700 or 27700; (3) the quantum yield is 0.85 or 0.81; and (4) the pH sensitivity of the fluorescent property is stable at pH 5 to 9; and a fluorescent protein derived from Fungia sp., which has the following properties: (1) the excitation maximum wavelength is 548 nm, and the fluorescence maximum wavelength is 561 nm; (2) the molar absorption coefficient at 548 nm is 75900 or 51000; (3) the quantum yield is 0.44 or 0.50; and (4) the pH sensitivity of the fluorescent property is pKa

摘要翻译： 本发明的目的是提供一种源自维他命A（Aequorea victoria）以外的生物体的新型荧光蛋白。 根据本发明，提供了一种衍生自真菌属的荧光蛋白，具有以下性质：（1）激发最大波长为455nm，荧光最大波长为488nm; （2）455nm处的摩尔吸光系数为38700或27700; （3）量子产率为0.85或0.81; 和（4）荧光性能的pH敏感性在pH5至9下是稳定的; 和一种来自真菌属的荧光蛋白，具有以下特性：（1）激发最大波长为548nm，荧光最大波长为561nm; （2）548nm处的摩尔吸光系数为75900或51000; （3）量子产率为0.44或0.50; 和（4）荧光性质的pH敏感度为pKa <5.0。

公开(公告)号：US20070207452A1

公开(公告)日：2007-09-06

申请号：US11729529

申请日：2007-03-29

申请人： Hirobumi Suzuki ,  Atsushi Miyawaki

发明人： Hirobumi Suzuki ,  Atsushi Miyawaki

IPC分类号： C12Q1/00 ,  C12Q1/68

CPC分类号： G01N33/5017

摘要： The present invention provides a method of detecting a micronucleus in a living cell to be measured, without having to fixate the cell, characterized by comprising generating a mother cell by introducing a gene encoding a single or a plurality of nucleus-related proteins and a gene encoding a fluorescence protein into a culture cell, and making then expressed in the cell, subjecting the cell to a treatment for a toxicity test or a mutagenicity test of a test substance, performing a limited excitation which makes a region of the micronucleus emit fluorescence limitedly, and quantitatively measuring an amount of the fluorescence corresponding to a component of the nucleus-related protein.

摘要翻译： 本发明提供一种检测待测细胞中的微核的方法，而不必固定细胞，其特征在于包括通过引入编码单个或多个核相关蛋白的基因和基因产生母细胞 将荧光蛋白编码到培养细胞中，然后在细胞中表达，对细胞进行毒性试验或试验物质的诱变试验处理，进行有限激发，使微核区域有限地发射荧光 并定量测定与核相关蛋白质的成分相对应的荧光量。

公开(公告)号：US20060275822A1

公开(公告)日：2006-12-07

申请号：US10538772

申请日：2003-12-10

申请人： Atsushi Miyawaki ,  Takeharu Nagai

发明人： Atsushi Miyawaki ,  Takeharu Nagai

IPC分类号： G01N33/53 ,  C12Q1/37 ,  C07H21/04 ,  C12P21/06 ,  C07K14/435

CPC分类号： G01N33/542 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/05 ,  C07K2319/06 ,  C07K2319/07 ,  C07K2319/09

摘要： It is an object of the present invention to provide a fluorescent indicator that is based on homo FRET (fluorescence resonance energy transfer) using fluorescent molecules with a small stokes' shift, thereby developing a visualization system for intermolecular interaction. The present invention provides a fluorescent indicator formed by fusing fluorescent molecular components having substantially identical fluorescent properties to the N- and C-terminal sides of a target sequence, to which an analytical substance binds or reacts, so as to change the three-dimensional structure of the indicator.

摘要翻译： 本发明的一个目的是提供一种基于使用具有小斯托克斯位移的荧光分子的同质FRET（荧光共振能量转移）的荧光指示剂，从而开发用于分子间相互作用的可视化系统。 本发明提供了一种荧光指示剂，其通过将具有基本相同荧光性质的荧光分子组分与目标序列的N-末端和末端侧融合而形成，分析物质结合或反应，从而改变三维结构 的指标。

公开(公告)号：US20060240472A1

公开(公告)日：2006-10-26

申请号：US10516317

申请日：2003-06-10

申请人： Atsushi Miyawaki ,  Satoshi Karasawa

发明人： Atsushi Miyawaki ,  Satoshi Karasawa

IPC分类号： G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C07K14/435

CPC分类号： G01N33/542 ,  C08L23/02

摘要： An object of the present invention is to provide a novel chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700 at 610 nm; and (3) the pH sensitivity of light-absorbing property is stable at between pH 4 and pH 10.

摘要翻译： 本发明的一个目的是提供一种衍生自日本n a属的新型生色蛋白。 本发明提供了具有以下特性的源自日本蝉的色素蛋白：（1）吸收最大波长为610nm，荧光不发射; （2）610 nm处的摩尔吸光系数为66,700; 和（3）在pH4和pH10之间，光吸收性质的pH敏感性是稳定的。

公开(公告)号：US20050106661A1

公开(公告)日：2005-05-19

申请号：US10492081

申请日：2002-10-10

申请人： Atsushi Miyawaki ,  Satoshi Karasawa ,  Toshio Araki

发明人： Atsushi Miyawaki ,  Satoshi Karasawa ,  Toshio Araki

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C07H21/04

CPC分类号： C07K14/43504

摘要： An object of the present invention is to provide a novel fluorescent protein derived from organisms other than Aequorea victoria. According to the present invention, there is provided a fluorescent protein derived from Galaxea fascicularis, which has the following properties: (1) the molecular weight is approximately 27,000; (2) a tetramer is formed in an equilibration state; (3) the excitation maximum wavelength is 492 nm, and the fluorescence maximum wavelength is 505 nm; (4) the molar absorption coefficient is 74,100; (5) the quantum yield is 0.625; and (6) the pH sensitivity of the fluorescent property is low in the range between pH 5 and pH 12.

摘要翻译： 本发明的目的是提供一种源自维他命A（Aequorea victoria）以外的生物体的新型荧光蛋白。 根据本发明，提供了一种衍生自Galaxea fascicularis的荧光蛋白，其具有以下性质：（1）分子量约为27,000; （2）在平衡状态下形成四聚体; （3）激发最大波长为492nm，荧光最大波长为505nm; （4）摩尔吸光系数为74,100; （5）量子产率为0.625; 和（6）pH5和pH12之间的荧光性能的pH敏感性低。