公开(公告)号：US20090191164A1

公开(公告)日：2009-07-30

申请号：US12353098

申请日：2009-01-13

申请人： Ravindra Majeti ,  Irving L. Weissman

发明人： Ravindra Majeti ,  Irving L. Weissman

IPC分类号： A61K35/12 ,  C12N5/08 ,  C12Q1/68 ,  C12Q1/02 ,  A61P37/00

CPC分类号： C12N5/0647 ,  A61K35/28 ,  A61K35/51 ,  G01N15/042 ,  G01N33/5073 ,  G01N2015/149 ,  G01N2500/00

摘要： A substantially enriched human multipotent progenitor cell population is provided, which is characterized as a progenitor cell capable of giving rise to the multipotent lineage but which lacks certain long-term self-renewal properties of the hematopoietic stem cell. Methods are provided for the isolation and culture of these cells. The cell enrichment methods employ reagents that specifically recognize CD34, CD38, CD90 and CD45RA, in conjunction with lineage specific markers. These cells give rise to all types of hematopoietic cells, e.g. myeloid and lymphoid cells, in vivo.

摘要翻译： 提供了一种基本上富集的人多能祖细胞群，其特征在于能够产生多能谱系但缺乏造血干细胞某些长期自我更新性质的祖细胞。 提供了用于分离和培养这些细胞的方法。 细胞富集方法使用特异性识别CD34，CD38，CD90和CD45RA的试剂，与谱系特异性标记物结合。 这些细胞产生所有类型的造血细胞，例如， 骨髓和淋巴细胞，体内。

公开(公告)号：US06465249B2

公开(公告)日：2002-10-15

申请号：US09766387

申请日：2001-01-18

申请人： Tannishtha Reya ,  Roeland Nusse ,  Irving L. Weissman

发明人： Tannishtha Reya ,  Roeland Nusse ,  Irving L. Weissman

IPC分类号： C12N500

CPC分类号： C12N5/0647 ,  C12N2501/125 ,  C12N2501/415 ,  C12N2501/998

摘要： Mammalian progenitor or stem cells are expanded in vitro by increasing the levels of &bgr;-catenin in the cell. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. The intracellular levels of &bgr;-catenin may be manipulated by providing exogenous &bgr;-catenin protein to the cell, or by introduction into the cell of a genetic construct encoding &bgr;-catenin. The &bgr;-catenin may be a wild-type or stabilized mutant form of the protein. Preferably the long term cell culture medium substantially lacks stromal cells and cytokines.

公开(公告)号：US5447852A

公开(公告)日：1995-09-05

申请号：US142897

申请日：1993-10-26

申请人： Jeffrey S. Friedman ,  Irving L. Weissman

发明人： Jeffrey S. Friedman ,  Irving L. Weissman

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61P43/00 ,  C07K14/705 ,  C07K16/00 ,  C07K16/28 ,  C07K19/00 ,  C12N1/21 ,  C12N9/90 ,  C12N15/12 ,  C12P21/02 ,  C12R1/19 ,  C12N5/10 ,  C12N15/61 ,  C12N15/62

CPC分类号： C07K14/705 ,  C12N9/90 ,  C07K2319/00 ,  C07K2319/23

摘要： The cloning and characterization of a new binding protein class, cyclophilin C (cyp C) and homologous proteins, is provided which is capable of binding to, e.g., the immunosuppressive drug cyclosporin A (CsA). The pattern of cyp C mRNA expression differs from that of cyp A mRNA expression, with cyp C being expressed in a more restricted subset of tissues, including those tissues reported to be most affected by CsA therapy. A fusion protein containing, e.g., amino acids 16-212 of cyp C has peptidyl-prolyl-isomerase activity (PPIase), and CsA inhibits this activity. Most significantly, these cyp C fusion proteins can be used as ligands for the identification of intracellular proteins which together form high affinity associations. For example, the cyp C fusion protein binds specifically to a protein of 77 Kd in the absence of CsA, while in the presence of CsA it no longer binds to this p77, but instead binds specifically to a protein 55 Kd, identified as calcineurin.

摘要翻译： 提供新的结合蛋白类，亲环蛋白C（cyp C）和同源蛋白的克隆和表征，其能够结合例如免疫抑制药物环孢菌素A（CsA）。 cyp C mRNA表达的模式与cyp A mRNA表达的模式不同，cyp C在更受限制的组织亚群中表达，包括报道最受CsA治疗影响的组织。 含有例如cyp C的氨基酸16-212的融合蛋白具有肽基 - 脯氨酰基异构酶活性（PPIase），并且CsA抑制该活性。 最显着的是，这些cypc融合蛋白可以用作鉴定细胞内蛋白质的配体，这些蛋白质共同形成高亲和力结合。 例如，cyp C融合蛋白在不存在CsA的情况下特异性结合77Kd的蛋白质，而在CsA存在下，其不再与该p77结合，而是特异性结合被鉴定为钙调神经磷酸的蛋白质55Kd。

公开(公告)号：US5362629A

公开(公告)日：1994-11-08

申请号：US740175

申请日：1991-08-05

申请人： Stuart L. Schreiber ,  Jeffrey S. Friedman ,  Irving L. Weissman ,  Jun Liu

发明人： Stuart L. Schreiber ,  Jeffrey S. Friedman ,  Irving L. Weissman ,  Jun Liu

IPC分类号： G01N33/94 ,  C12Q1/42 ,  A61K37/02 ,  C12Q1/00 ,  G01N33/53

CPC分类号： G01N33/9493 ,  G01N2500/20 ,  Y10S514/885

摘要： A method of evaluating the immunosuppressive activity of a compound including contacting the compound with calcineurin and determining the ability of the compound to bind to the calcineurin. The ability to bind to the calcineurin is positively correlated to the immunosuppressive activity of the compound.

摘要翻译： 评估化合物的免疫抑制活性的方法，包括使化合物与钙调神经磷酸酶接触并测定化合物结合钙调神经磷酸的能力。 结合钙调神经磷酸的能力与化合物的免疫抑制活性呈正相关。

公开(公告)号：US5213977A

公开(公告)日：1993-05-25

申请号：US917783

申请日：1992-07-20

申请人： Irving L. Weissman ,  Howard K. Gershenfeld

发明人： Irving L. Weissman ,  Howard K. Gershenfeld

IPC分类号： A61K38/00 ,  C07K16/40 ,  C12N9/64

CPC分类号： C12N9/6467 ,  C07K16/40 ,  A61K38/00

摘要： A human serine protease expressed in cytotoxic killer cells, having a mass of about 25.8kD, and having the amino acid residues of the serine protease charge-relay catalytic mechanism conserved is provided. The protease can be produced by recombinant DNA technology. The cDNA is also provided.

摘要翻译： 提供了在细胞毒性杀伤细胞中表达的人丝氨酸蛋白酶，具有约25.8kD的质量，并且具有保守的丝氨酸蛋白酶电荷继电器催化机制的氨基酸残基。 蛋白酶可以通过重组DNA技术生产。 还提供cDNA。