公开(公告)号：US20240285686A1

公开(公告)日：2024-08-29

申请号：US18571575

申请日：2022-06-15

Applicant: WEST CHINA HOSPITAL, SICHUAN UNIVERSITY

Inventor： Xudong ZHAO ,  Bin SUN

IPC: A61K35/17 ,  A61K39/00 ,  A61P35/04 ,  C07K14/725 ,  C12N5/0783 ,  C12N5/10 ,  C12N9/64 ,  C12N15/86

CPC classification number: A61K35/17 ,  A61K39/4611 ,  A61K39/4631 ,  A61K39/464476 ,  A61P35/04 ,  C07K14/7051 ,  C12N5/0636 ,  C12N5/10 ,  C12N9/6467 ,  C12N15/86 ,  A61K2239/15 ,  A61K2239/17 ,  A61K2239/21 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2510/00 ,  C12N2740/15043 ,  C12Y304/21079

Abstract: A preparation and an application of a chimeric antigen receptor immune cell constructed on the basis of granzyme B are provided, including, a granzyme B (GrB)-based modified chimeric antigen receptor (CAR), the CAR containing an extracellular binding domain, and the extracellular binding domain being capable of specifically targeting heat shock proteins. The CAR immune cell acts by means of binding of a ligand and a receptor, and has relatively high targeting specificity and relatively better safety.

公开(公告)号：US20230203467A1

公开(公告)日：2023-06-29

申请号：US18052476

申请日：2022-11-03

Applicant: Research Development Foundation

Inventor： Michael G. ROSENBLUM ,  Khalid Amanali MOHAMEDALI ,  Lawrence H. CHEUNG

IPC: C12N9/64 ,  C07K14/47 ,  C07K14/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30

CPC classification number: C12N9/6467 ,  C07K14/4747 ,  C12N9/6424 ,  C07K14/001 ,  C07K16/00 ,  C07K16/2863 ,  C07K16/30 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/30 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/55 ,  C07K2319/10 ,  C07K2319/03

Abstract: Cell-targeted serine protease constructs are provided. Such constructs can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity. Methods and compositions for treating lapatinib or trastuzumab-resistant cancers are also provided.

公开(公告)号：US09944706B2

公开(公告)日：2018-04-17

申请号：US14440947

申请日：2013-11-04

Applicant: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V

Inventor： Stefan Barth ,  Sonja Schiffer

IPC: C12N5/00 ,  C12N15/00 ,  C07K16/00 ,  C07K16/28 ,  C12N9/64 ,  C12N9/76

CPC classification number: C07K16/283 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/2878 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/55 ,  C12N9/6427 ,  C12N9/6467

Abstract: The technology provided herein relates to novel immunoproteases suitable to induce apotosis in selected diseased target cells, comprising the serine protease granzyme M, and methods for using such cytolytic fusion proteins for the treatment of various diseases, in particular for the treatment of cancer.

公开(公告)号：US20170260517A1

公开(公告)日：2017-09-14

申请号：US15434648

申请日：2017-02-16

Applicant: Research Development Foundation

Inventor： Xinjian LIN ,  Xiying SHANG ,  Stephen B. HOWELL

IPC: C12N9/64 ,  C07K14/435 ,  C07K14/59 ,  C12N9/96

CPC classification number: C12N9/6467 ,  A61K47/65 ,  A61K47/6889 ,  A61K48/00 ,  C07K14/43504 ,  C07K14/59 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/33 ,  C07K2319/40 ,  C07K2319/55 ,  C07K2319/70 ,  C07K2319/74 ,  C07K2319/90 ,  C12N9/52 ,  C12N9/6424 ,  C12N9/96 ,  C12Y304/21079 ,  C12Y304/22007 ,  C12Y304/2207

Abstract: Cell-targeted cytotoxic agents, including sortase serine protease constructs, are provided. Such compounds can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant sortase serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity.

公开(公告)号：US20170182096A1

公开(公告)日：2017-06-29

申请号：US15301343

申请日：2015-04-15

Applicant: BRITISH COLUMBIA CANCER AGENCY BRANCH

Inventor： Robert Holt ,  Daniel Woodsworth

IPC: A61K35/17 ,  A61K38/45 ,  C12N5/0783 ,  C12N9/64 ,  C12N9/24 ,  C12N9/10 ,  A61K38/47 ,  A61K38/48

CPC classification number: A61K35/17 ,  A61K38/164 ,  A61K38/168 ,  A61K38/45 ,  A61K38/47 ,  A61K38/48 ,  A61K38/482 ,  C07K14/7051 ,  C07K2319/55 ,  C12N5/0646 ,  C12N9/1077 ,  C12N9/2497 ,  C12N9/6467 ,  C12N2510/00 ,  C12N2740/15043 ,  C12Y204/02036 ,  C12Y302/02022 ,  C12Y304/21079

Abstract: The invention is directed to methods and compositions for cell-based targeted delivery of predetermined compounds to a population of target cells. In some embodiments, methods of the invention include providing cytotoxic lymphocytes genetically modified to produce and sequester in lytic granules fusion proteins comprising a granzyme, or other effector agent, and a predetermined protein, so that upon specific contact of the cytotoxic lymphocytes with the target cells, the granzyme-perforin pathway of the cytotoxic lymphocytes is activated, leading to the delivery of the fusion protein to the cytosols of the target cells.

公开(公告)号：US20150329847A1

公开(公告)日：2015-11-19

申请号：US14716275

申请日：2015-05-19

Applicant: Samsung Electronics Co., Ltd.

Inventor： Jae Il Lee ,  Hye Yoon Kang ,  Dongkyu Shin ,  Jung Min Kim ,  Jungmin Lee

IPC: C12N9/64 ,  C07K14/00 ,  A61K38/03 ,  C07K4/00 ,  A61K38/16 ,  A61K39/395 ,  A61K38/48 ,  C07K16/28

CPC classification number: A61K38/03 ,  A61K38/00 ,  A61K38/482 ,  A61K39/3955 ,  C07K4/00 ,  C07K16/2863 ,  C07K2318/20 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/33 ,  C07K2319/50 ,  C07K2319/70 ,  C12N9/6467 ,  C12Y304/21079

Abstract: A fusion protein including granzyme B, a cell penetrating peptide, a cleavage site, and a targeting moiety, a composition for cell membrane penetration comprising the fusion protein, and an anticancer composition comprising the fusion protein.

Abstract translation: 包含粒酶B，细胞穿透肽，切割位点和靶向部分的融合蛋白，包含融合蛋白的细胞膜渗透用组合物和包含该融合蛋白的抗癌组合物。

公开(公告)号：US20120252097A1

公开(公告)日：2012-10-04

申请号：US13281004

申请日：2011-10-25

Applicant: Michael G. Rosenblum ,  Yuying Liu

Inventor： Michael G. Rosenblum ,  Yuying Liu

IPC: C12N9/64 ,  C12N1/21 ,  C12N5/10 ,  C12N15/63

CPC classification number: C12N9/6467 ,  A61K38/00 ,  C07K14/4747 ,  C07K14/52 ,  C07K2319/00

Abstract: The present invention relates to targeted killing of a cell utilizing a chimeric polypeptide comprising a cell-specific targeting moiety and a signal transduction pathway factor. In a preferred embodiment, the signal transduction pathway factor is an apoptosis-inducing factor, such as granzyme B, granzyme A, or Bax.

Abstract translation: 本发明涉及利用包含细胞特异性靶向部分和信号转导途径因子的嵌合多肽靶向杀死细胞。 在优选的实施方案中，信号转导途径因子是凋亡诱导因子，例如颗粒酶B，粒酶A或Bax。

公开(公告)号：US07759091B2

公开(公告)日：2010-07-20

申请号：US12040111

申请日：2008-02-29

Applicant: Michael G. Rosenblum ,  Yuying Liu

Inventor： Michael G. Rosenblum ,  Yuying Liu

IPC: C12P21/06 ,  C07K14/00 ,  C07K16/00 ,  C12N9/00

CPC classification number: C12N9/6467 ,  A61K38/00 ,  C07K14/4747 ,  C07K14/52 ,  C07K2319/00

Abstract: The present invention relates to targeted killing of a cell utilizing a chimeric polypeptide comprising a cell-specific targeting moiety and a signal transduction pathway factor. In a preferred embodiment, the signal transduction pathway factor is an apoptosis-inducing factor, such as granzyme B, granzyme A, or Bax.

Abstract translation: 本发明涉及利用包含细胞特异性靶向部分和信号转导途径因子的嵌合多肽靶向杀死细胞。 在优选的实施方案中，信号转导途径因子是凋亡诱导因子，例如颗粒酶B，粒酶A或Bax。

公开(公告)号：US20060280749A1

公开(公告)日：2006-12-14

申请号：US11415342

申请日：2006-05-01

Applicant: Michael Rosenblum ,  Yuying Liu

Inventor： Michael Rosenblum ,  Yuying Liu

IPC: A61K39/395 ,  C07H21/04 ,  C12P21/06 ,  C07K16/46 ,  C07K16/30

CPC classification number: C12N9/6467 ,  A61K38/00 ,  C07K14/4747 ,  C07K14/52 ,  C07K2319/00

Abstract: The present invention relates to targeted killing of a cell utilizing a chimeric polypeptide comprising a cell-specific targeting moiety and a signal transduction pathway factor. In a preferred embodiment, the signal transduction pathway factor is an apoptosis-inducing factor, such as granzyme B, granzyme A, or Bax.

公开(公告)号：US06537784B1

公开(公告)日：2003-03-25

申请号：US09229151

申请日：1999-01-12

Applicant: Revati J. Tatake ,  Steven D. Marlin ,  Randall W. Barton

Inventor： Revati J. Tatake ,  Steven D. Marlin ,  Randall W. Barton

IPC: C12P1934

CPC classification number: C12N9/6467 ,  A61K38/00 ,  A61K48/00 ,  C07K14/525 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2830/85

Abstract: This invention relates to chimeric nucleic acids and to the therapeutic induction of apoptosis in activated inflammatory cells, or cells at a site of inflammation, by introducing into those cells the chimeric nucleic acid. The chimeric nucleic acid having at least one TNF&agr; promoter enhancer attached to a functional copy of a TNF&agr; promoter and further attached to at least one copy of an apoptosis-inducing gene, which is further attached to a 3′UTR. The apoptosis-inducing gene is Granzyme B. The invention also relates to methods of making and using self-regulated apoptosis chimeric nucleic acids and pharmaceutical compositions containing them for treating inflammatory diseases.

Abstract translation: 本发明涉及嵌合核酸和通过将嵌合核酸引入那些细胞中的活化炎症细胞或炎症部位的细胞的细胞凋亡的治疗诱导。 具有至少一个TNFalpha启动子增强子的嵌合核酸与TNFalpha启动子的功能拷贝连接，并进一步连接至凋亡诱导基因的至少一个拷贝，其进一步连接到3'UTR。 凋亡诱导基因是颗粒酶B.本发明还涉及制备和使用自我调节的凋亡嵌合核酸的方法和含有它们的药物组合物用于治疗炎症性疾病。