公开(公告)号：US5981702A

公开(公告)日：1999-11-09

申请号：US531439

申请日：1995-09-21

Applicant: Hui Zhang ,  David Beach

Inventor： Hui Zhang ,  David Beach

IPC: A61K48/00 ,  C07K14/47 ,  C12N15/12 ,  C06K1/00

CPC classification number: C07K14/4738 ,  A01K2217/05 ,  A61K48/00 ,  C07K2319/00

Abstract: The present invention relates to the discovery in mammalian cells, particularly human cells, of novel S-phase kinase associated proteins, p19 and p45, referred to herein as "Skp". As described herein, these proteins are components of the tumor cell-specific cyclin A/CDK2 complex and function to facilitate DNA replication. Interference with p45 function in vivo prevented entry into S-phase in both normal and transformed cells. Binding data indicated that p45 and p19 associate with each other in a binary complex. Moreover, p45 is required for p19 binding to cyclin A/CDK2.

Abstract translation: 本发明涉及在哺乳动物细胞，特别是人类细胞中发现的新型S期激酶相关蛋白p19和p45，在本文中称为“Skp”。 如本文所述，这些蛋白质是肿瘤细胞特异性细胞周期蛋白A / CDK2复合物的组分，并且有助于DNA复制。 与体内p45功能的干扰阻止了在正常细胞和转化细胞中进入S期。 绑定数据表明p45和p19在二元复合体中彼此相关联。 此外，p19是p19与细胞周期蛋白A / CDK2结合所必需的。

公开(公告)号：US5951979A

公开(公告)日：1999-09-14

申请号：US144925

申请日：1998-09-01

Applicant: Nicholas Tonks ,  Andrew J. Flint

Inventor： Nicholas Tonks ,  Andrew J. Flint

IPC: C12N15/09 ,  A61K38/00 ,  A61K38/46 ,  A61P35/00 ,  C12N9/12 ,  C12N9/16 ,  C12Q1/42

CPC classification number: C12N9/16 ,  A61K38/00 ,  C12N2799/026

Abstract: Novel protein tyrosine phosphatases in which the invariant aspartate residue is replaced with an alanine residue and which bind to a tyrosine phosphorylated substrate and are catalytically attenuated are described. Also described are methods of identifying tyrosine phosphorylated proteins which complex with the described protein tyrosine phosphatases.

Abstract translation: 描述了新的蛋白酪氨酸磷酸酶，其中不变的天冬氨酸残基被丙氨酸残基替代并结合酪氨酸磷酸化底物并被催化衰减。 还描述了鉴定与所述蛋白质酪氨酸磷酸酶复合的酪氨酸磷酸化蛋白质的方法。

公开(公告)号：US5929223A

公开(公告)日：1999-07-27

申请号：US319866

申请日：1994-10-07

Applicant: Timothy P. Tully ,  Jerry Chi-Ping Yin ,  Michael Regulski

Inventor： Timothy P. Tully ,  Jerry Chi-Ping Yin ,  Michael Regulski

IPC: G01N33/15 ,  A61K38/00 ,  A61P25/28 ,  A61P37/02 ,  C07K7/08 ,  C07K14/435 ,  C07K14/47 ,  C12N9/02 ,  C12N15/09 ,  C12N15/12 ,  C07H21/04

CPC classification number: C12N9/0075 ,  C07K14/435 ,  C07K14/4703 ,  C07K14/4705 ,  A01K2217/05 ,  A61K38/00 ,  C07K2319/00 ,  Y10T436/105831

Abstract: A method of regulating long term memory is disclosed. Also disclosed is isolated DNA encoding a cyclic 3',5'-adenosine monophosphate responsive transcriptional activator, isolated DNA encoding an antagonist of cyclic 3',5'-adenosine monophosphate-inducible transcription, isolated DNA encoding an enhancer-specific activator, and isolated DNA encoding a nitric oxide synthase.

Abstract translation: 公开了一种调节长期记忆的方法。 还公开了编码环状3'，5'-腺苷单磷酸应答转录活化剂的分离的DNA，编码环状3'，5'-腺苷一磷酸诱导型转录的拮抗剂的分离的DNA，编码增强子特异性激活物的分离的DNA， 编码一氧化氮合酶的DNA。

公开(公告)号：US5527896A

公开(公告)日：1996-06-18

申请号：US688352

申请日：1991-04-19

Applicant: Michael H. Wigler ,  John J. Colicelli

Inventor： Michael H. Wigler ,  John J. Colicelli

IPC: G01N33/50 ,  C07K14/00 ,  C07K14/82 ,  C12N1/19 ,  C12N9/14 ,  C12N9/16 ,  C12N15/09 ,  C12N15/12 ,  C12N15/55 ,  C12N15/81 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/865 ,  C07H21/04

CPC classification number: C07K14/47 ,  C07K14/82 ,  C12N15/81 ,  C12N9/16

Abstract: Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.

公开(公告)号：US5041376A

公开(公告)日：1991-08-20

申请号：US282165

申请日：1988-12-09

Applicant: Mary J. Gething ,  Joseph F. Sambrook ,  Patricia Gallagher

Inventor： Mary J. Gething ,  Joseph F. Sambrook ,  Patricia Gallagher

IPC: C07K14/11 ,  C07K16/10 ,  C12N9/72 ,  C12N15/00

CPC classification number: C07K14/005 ,  C07K16/1018 ,  C12N15/00 ,  C12N9/6459 ,  C12Y304/21069 ,  C12N2760/16022

Abstract: The present invention relates to a method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells (transportable proteins) by the addition of supernumerary N-linked oligosaccharide side chains at chosen sites on the surface thereof using oligonucleotide mutagenesis. The present invention also relates to mutant transportable proteins having supernumerary N-linked oligosaccharide side chains which shield functional sites or epitopes; and genes which encode the same.

Abstract translation: 本发明涉及通过使用寡核苷酸诱变在其表面上的选定位点添加超数N-连接的寡糖侧链来鉴定或屏蔽进入真核细胞（可运输蛋白质）胞外途径的蛋白质的功能位点或表位的方法 。 本发明还涉及具有屏蔽功能位点或表位的超数N-连接寡糖侧链的突变型可运输蛋白质; 和编码相同的基因。

公开(公告)号：US4866166A

公开(公告)日：1989-09-12

申请号：US647881

申请日：1984-08-31

Applicant: Michael Wigler

Inventor： Michael Wigler

IPC: C07K16/32 ,  C12Q1/02

CPC classification number: C07K16/32 ,  C12Q1/02

Abstract: A sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice is disclosed. Genes mcf2 and mcf3 have been detected in tissue breast cancer using this assay.

Abstract translation: 公开了一种基于裸转染的NIH3T3细胞的致瘤性转化基因的敏感生物测定法。 使用该测定法在组织乳腺癌中检测到mcf2和mcf3基因。

公开(公告)号：US12215128B2

公开(公告)日：2025-02-04

申请号：US17261313

申请日：2019-07-19

Applicant: COLD SPRING HARBOR LABORATORY ,  DEPYMED, INC.

Inventor： Nicholas Tonks ,  Navasona Krishnan ,  Andreas Grill

IPC: C07D213/38 ,  C07D401/12 ,  C07J43/00

Abstract: Compounds of formula: are described herein. The compounds selectively complex copper and are therefore useful both abiotically for measuring and detecting small amounts of copper and, in biological systems, for treating diseases associated with inappropriate copper levels, such as Wilson's disease and gastric cancer.

公开(公告)号：US20250011765A1

公开(公告)日：2025-01-09

申请号：US18649873

申请日：2024-04-29

Applicant: Duke University ,  Cold Spring Harbor Laboratory

Inventor： Z. Josh Huang ,  Yongjun Qian

IPC: C12N15/11 ,  A61P25/08 ,  A61P25/28

Abstract: A readrRNA (RNA sensing by Endogenous ADAR) molecule is a modular RNA molecule that facilitates sensing and detection of a cell type or cell status of a cell, including a cell of a mammalian nervous system, such as a neuronal or non-neuronal cell of the mammalian central and/or peripheral nervous system, and/or facilitates delivery of an effector protein to the selected cell. A composition that includes such a modular RNA molecule and another nucleic acid (linked or unlinked to the modular RNA molecule) is a CellREADR (Cell access through RNA sensing by Endogenous ADAR). CellREADR senses the presence of a selected cell RNA in a cell of a mammalian nervous system via readrRNA and leverages RNA editing mediated by ADAR (adenosine deaminase acting on RNA) for coupling the detection of a cell-defining RNA with translation of one or more effector proteins in a cell of a mammalian nervous system.

公开(公告)号：US20230270067A1

公开(公告)日：2023-08-31

申请号：US18001370

申请日：2021-06-09

Applicant: University of Georgia Research Foundation, Inc. ,  Cold Spring Harbor Laboratory

Inventor： R. Kelly Dawe ,  David Jackson

IPC: A01H1/08 ,  C12N15/11 ,  C12N9/22 ,  C12N15/82 ,  C12Q1/6895 ,  C07K14/415

CPC classification number: A01H1/08 ,  C07K14/415 ,  C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12Q1/6895 ,  C12N2310/20 ,  C12N2800/80 ,  C12Q2600/13

Abstract: Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant’s genome are also provided.

公开(公告)号：US11660306B2

公开(公告)日：2023-05-30

申请号：US16377875

申请日：2019-04-08

Applicant: Cold Spring Harbor Laboratory

Inventor： Nicholas Tonks ,  Navasona Krishnan

IPC: A61K31/662 ,  C07F9/38 ,  C12Q1/6883

CPC classification number: A61K31/662 ,  C07F9/3808 ,  C12Q1/6883 ,  C12Q2600/156

Abstract: The present invention relates to agents and methods for treating autism spectrum disorders, such as Rett Syndrome.