公开(公告)号：US20030059778A1

公开(公告)日：2003-03-27

申请号：US09962288

申请日：2001-09-24

Inventor： Andrew Berlin ,  Steven J. Kirch ,  Gabi Neubauer ,  Valluri Rao ,  Mineo Yamakawa

IPC: C12Q001/68 ,  G01J003/44

CPC classification number: G01N21/6428 ,  B01L3/5027 ,  C12Q1/6869 ,  G01J3/4406 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/6441 ,  G01N2021/651 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656 ,  C12Q2565/632 ,  C12Q2565/101 ,  C12Q2533/101 ,  C12Q2565/629 ,  C12Q2565/60

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

Abstract translation: 本文公开的方法，组合物和装置用于核酸序列测定。 所述方法包括使用DNA或RNA聚合酶或类似的合成试剂分离一个或多个核酸模板分子和核酸的新生互补链的聚合。 当新生链一次延伸一个核苷酸时，通过拉曼光谱法或FRET监测核苷酸前体从溶液中的消失。 新生链的核酸序列和模板链的互补序列可以通过跟踪聚合反应期间核苷酸前体的掺入顺序来确定。 某些实施例涉及包括在实施所要求保护的方法中使用的反应室和检测单元的装置。 方法，组合物和装置在用于在单次测序反应中测序非常长的核酸模板中有用。

公开(公告)号：US20030007145A1

公开(公告)日：2003-01-09

申请号：US10186573

申请日：2002-07-01

Applicant: Olympus Optical Co., Ltd.

Inventor： Yoshihiro Shimada

IPC: G01J003/44

CPC classification number: G02B21/002

Abstract: A laser microscope includes a laser light emitting system which emits a pump beam and a Stokes beam having different frequencies, and a common optical fiber having one end and the other end. The pump beam and the Stokes beam emitted from the laser light emitting system are incident on the one end thereof and emitted from the other end thereof. A beam irradiating mechanism condenses and irradiates the pump beam and the Stokes beam emitted from the other end of the optical fiber, onto a sample via the objective lens, thereby making an anti-Stokes beam be emitted from the sample. A photo detector detects the anti-Stokes beam emitted from the sample. A beam selecting mechanism allows only the anti-Stokes beam of the pump beam, the Stokes beam and the anti-Stokes beam to be received on the photo detector.

Abstract translation: 激光显微镜包括发射泵浦光束的激光发射系统和具有不同频率的斯托克斯光束，以及具有一端和另一端的公共光纤。 从激光发射系统发射的泵浦光束和斯托克斯光束入射到其一端并从其另一端射出。 光束照射机构将从光纤的另一端发射的泵浦光束和斯托克斯光束经由物镜冷凝并照射到样品上，从而从样品发射反斯托克斯光束。 光电检测器检测从样品发射的反斯托克斯光束。 光束选择机构仅允​​许泵光束的反斯托克斯光束，斯托克斯光束和反斯托克斯光束被接收在光检测器上。

公开(公告)号：US20020186370A1

公开(公告)日：2002-12-12

申请号：US09877531

申请日：2001-06-08

Applicant: Motorola, Inc., Semiconductor 300 GmbH & Co.KG and Infineon Technologies AG.

Inventor： Michael Roesner ,  Manfred Schneegans ,  David Wallis

IPC: G01J003/44 ,  G01N021/88

CPC classification number: B24B37/005 ,  B23Q17/0909 ,  B23Q17/24 ,  B24B21/04 ,  B24B27/0683 ,  B24B49/12 ,  B24B49/183 ,  G01N21/65

Abstract: A machining apparatus (10) comprises a material removing tool (12) movably mounted for removing material from a workpiece (14); means for illuminating (42, 54) a sample area upon a tool surface (34) with excitation radiation; means for receiving (42, 54) sample light emitted from the sample area; a spectral analyzer (54) for performing a spectral analysis of the sample light received; and means for determining (60) the condition of the tool at the sample area from the spectral analysis of the sample light. The wear of the tool (12) is determined as such a condition. Operation parameters of the machining apparatus (10) are adjusted according to the determined wear. An example application is a wafer dicing tool.

Abstract translation: 一种加工设备（10）包括可移动地安装以从工件（14）去除材料的材料去除工具（12）。 用于用激发辐射照射（42,44）在工具表面（34）上的样品区域的装置; 用于接收（42,54）从样品区域发射的样品光的装置; 用于对所接收的样本光进行光谱分析的光谱分析器（54）; 以及用于从样品光的光谱分析确定（60）样品区域中的工具的状态的装置。 刀具（12）的磨损被确定为这样的条件。 根据所确定的磨损来调整加工装置（10）的操作参数。 示例应用是晶片切割工具。

公开(公告)号：US20020163639A1

公开(公告)日：2002-11-07

申请号：US09848892

申请日：2001-05-04

Inventor： Kenneth E. Stephenson

IPC: G01J003/44

CPC classification number: G01N21/658 ,  G01J3/44

Abstract: A method of and apparatus for determining a physical property of a material. The method includes: attaching nanoparticles to a substrate; positioning the substrate near the material; illuminating the nanoparticles with photons having wavelengths that stimulate surface enhanced Raman emissions; detecting photons emitted as a result of the illumination; and determining said physical property of said material using said detected photons. The apparatus includes: a substrate; nanoparticles attached to the substrate; a light source, connected to the substrate, for illuminating the nanoparticles with photons having wavelengths that stimulate surface enhanced Raman emissions; a photodetector, connected to the substrate, for detecting photons emitted as a result of illumination of the nanoparticles; and a processor, connected to the photodetector, for determining a property of material near the nanoparticles from the detected photons. The inventive method and apparatus are particularly adapted for use in connection with hydrocarbon exploration and production activities.

Abstract translation: 用于确定材料的物理性质的方法和装置。 该方法包括：将纳米颗粒附着到基底上; 将基板定位在材料附近; 用具有刺激表面增强拉曼发射的波长的光子照射纳米颗粒; 检测作为照明结果发射的光子; 以及使用所述检测到的光子确定所述材料的所述物理性质。 该装置包括：基板; 附着于底物的纳米颗粒; 连接到衬底的光源，用于用具有刺激表面增强拉曼发射的波长的光子照射纳米颗粒; 连接到基板的光电检测器，用于检测作为纳米颗粒的照明结果发射的光子; 以及连接到光电检测器的处理器，用于从检测到的光子确定纳米颗粒附近的材料的性质。 本发明的方法和装置特别适用于与碳氢化合物勘探和生产活动有关的用途。

公开(公告)号：US20020048013A1

公开(公告)日：2002-04-25

申请号：US09908453

申请日：2001-07-18

Applicant: Wizard of Ink & Co.

Inventor： Francis A. Nottke ,  James A. Kirchoff ,  Jonathan N. Lucke ,  John Pollard

IPC: G01J003/44 ,  G06K009/74

CPC classification number: G01J3/44 ,  G01N21/65 ,  G07D7/1205

Abstract: The LVARS is a fully instrumental, non-destructive spectroscopic device for the analysis and verification and authentication of the optical and electromagnetic properties (OEMP) of the inks, dyes, thin films, plastics, toners, paper, fixatives, paints, and printing agents used in documents and financial instruments. The instrument is quantitative in nature so as to correlate compositional data (elemental, isotopic, structure) with Raman optical spectra. The LVARS design consists of a computer-controlled spectrometer with a microscope-guided grid head containing the laser excitation source and detector and optics.

Abstract translation: LVARS是用于分析和验证油墨，染料，薄膜，塑料，调色剂，纸张，固定剂，油漆和印刷剂的光学和电磁特性（OEMP）的完全仪器，非破坏性光谱仪器。 用于文件和金融工具。 仪器本质上是定量的，以便将组成数据（元素，同位素，结构）与拉曼光谱相关联。 LVARS设计由计算机控制的光谱仪与包含激光源和检测器和光学器件的显微镜导向栅格头组成。

公开(公告)号：US20010053521A1

公开(公告)日：2001-12-20

申请号：US09815909

申请日：2001-03-23

Inventor： David I. Kreimer ,  Oleg A. Yevin ,  Thomas H. Nufert

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  G01J003/44

CPC classification number: G01N33/553 ,  B01J13/0008 ,  B82B1/00 ,  B82Y30/00 ,  F24S10/90 ,  F24S70/10 ,  F28D15/02 ,  G01N21/65 ,  G01N21/658 ,  G01N33/54346 ,  G01N33/54366 ,  G01N33/54373 ,  Y02E10/40

Abstract: This invention comprises devices, compositions and methods for detecting analytes in complex solutions. In certain embodiments of this invention, the receptor molecules can be lacking in a Raman signal generating moiety that is present in an analyte molecule, so that binding of the analyte to the receptor provides the array with the Raman signal generating moiety. In other embodiments, binding of a receptor to an analyte is detected by observing an alteration or shift of Raman spectral features upon forming of an analyte-receptor associate. Enzymes can be detected by binding to receptors comprising materials that bind to the active site of the enzyme. Antibodies can be detected by formation of an antibody-antigen complex, wherein the antigen is part of a receptor. Analytes that can be detected using these methods include nucleic acids, proteins, and other molecules that can specifically bind to the arrays. Particle structures are disclosed that enhance the Raman a signal produced by an analyte through surface and resonance phenomena. Novel methods are presented for manufacturing particle structures and for attaching receptor molecules to areas of enhanced resonance on the particle structures, thereby producing Raman signals that can be used for highly specific, sensitive assays of biological and other molecules of interest.

Abstract translation: 本发明包括用于检测复杂溶液中分析物的装置，组合物和方法。 在本发明的某些实施方案中，受体分子可能缺乏存在于分析物分子中的拉曼信号产生部分，使得分析物与受体的结合为阵列提供拉曼信号产生部分。 在其它实施方案中，通过在形成分析物 - 受体缔合物时观察拉曼光谱特征的改变或偏移来检测受体与分析物的结合。 可以通过结合包含结合酶的活性位点的材料的受体来检测酶。 可以通过形成抗体 - 抗原复合物来检测抗体，其中抗原是受体的一部分。 可以使用这些方法检测的分析物包括可以特异性结合阵列的核酸，蛋白质和其他分子。 公开了通过表面和共振现象增强由分析物产生的信号的拉曼的粒子结构。 提出了用于制造颗粒结构和将受体分子附着在颗粒结构上增强共振区域的新方法，从而产生可用于生物和其他分子分子的高度特异性，敏感测定的拉曼信号。

公开(公告)号：US20040263843A1

公开(公告)日：2004-12-30

申请号：US10826706

申请日：2004-04-16

Inventor： Kevin J. Knopp ,  Daryoosh Vakhshoori

IPC: G01J003/44

CPC classification number: G01J3/44 ,  G01N21/65

Abstract: A Raman spectroscopy system includes a laser source for providing a laser beam, and an optical probe assembly including a photonic crystal fiber light guide for receiving the laser beam from the laser source and for directing the laser beam toward a specimen of selected material, a lens for receiving the laser beam in the light guide and directing the beam onto the specimen and for receiving reflected light from the specimen and directing the reflected light back through the fiber light guide, and a dichroic beam splitter for directing a portion of the reflected beam out of the optical probe assembly. The system further includes an optical spectrum analyzer for receiving the portion of the reflected beam and for exhibiting a Raman signature of the specimen.

Abstract translation: 拉曼光谱系统包括用于提供激光束的激光源，以及光探头组件，其包括光子晶体光纤导向器，用于接收来自激光源的激光束并将激光束引向所选材料的样本， 用于在光导中接收激光束并将光束引导到样本上并且用于接收来自样本的反射光并将反射光引导回穿过光纤导光体，以及二向色分束器，用于将一部分反射光束引出 的光学探头组件。 该系统还包括光谱分析仪，用于接收反射光束的一部分并且用于展示样品的拉曼特征。

公开(公告)号：US20040239921A1

公开(公告)日：2004-12-02

申请号：US10826954

申请日：2004-04-15

Inventor： Manfred Schneegans ,  Frank Pietzschmann

IPC: G01J003/44 ,  G01R031/02

CPC classification number: G01R1/06761 ,  G01R1/06738 ,  G01R3/00

Abstract: A probe needle for testing semiconductor chips includes one end that is fixed in a holding element and a free end that includes a contact tip. The probe needle is providednullat least on the surface of the contact tipnullwith a layer consisting of a chemically inert, electroconductive material which is hard in relation to the material of contact surfaces of the semiconductor chips. For example, the layer can be titanium nitride.

Abstract translation: 用于测试半导体芯片的探针包括固定在保持元件中的一端和包括接触尖端的自由端。 探针至少设置在接触尖端的表面上 - 由与化学惰性的导电材料组成的层相对于半导体芯片的接触表面的材料是硬的。 例如，该层可以是氮化钛。

公开(公告)号：US20040189989A1

公开(公告)日：2004-09-30

申请号：US10823902

申请日：2004-04-14

Inventor： Charles W. Gardner JR. ,  John S. Maier ,  Matthew P. Nelson ,  Robert C. Schweitzer ,  Patrick J. Treado ,  G. Steven Vanni ,  Juliane Wolfe

IPC: G01J003/44 ,  G01N021/65

CPC classification number: G01N21/6486 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/2823 ,  G01J3/44 ,  G01N21/31 ,  G01N21/33 ,  G01N21/359 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/65 ,  G01N2021/1736 ,  G01N2021/6421 ,  G01N2021/6423 ,  G01N2021/656

Abstract: Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms.

公开(公告)号：US20040179195A1

公开(公告)日：2004-09-16

申请号：US10387080

申请日：2003-03-12

Inventor： Xing Su ,  Lei Sun ,  Tae-Woong Koo ,  Selena Chan

IPC: G01J003/44 ,  G01N021/65

CPC classification number: G01N21/658 ,  B82Y10/00 ,  B82Y20/00

Abstract: Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

Abstract translation: 简而言之，根据本发明的一个实施例，可以通过使用氯化锂作为增强剂激活用于表面增强拉曼光谱的金属结构来增加来自表面增强拉曼光谱的信号的强度。 增加的信号强度可以允许使用表面增强拉曼光谱来检测单个分析物例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。