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公开(公告)号：US20190177699A1
公开(公告)日：2019-06-13
申请号：US16278019
申请日：2019-02-15
发明人： Hironori OMURA , Hirokazu SANDA , Takako YADA , Tetsunari MORITA , Mika KUYAMA , Tokuji IKEDA , Kenji KANO , Seiya TSUJIMURA
摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.
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公开(公告)号：US20190062798A1
公开(公告)日：2019-02-28
申请号：US16145178
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12Q1/32 , C12N9/02
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190048384A1
公开(公告)日：2019-02-14
申请号：US16145170
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040442A1
公开(公告)日：2019-02-07
申请号：US16145184
申请日：2018-09-28
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/02 , C12N9/04 , C12Q1/32 , G01N27/327
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190040437A1
公开(公告)日：2019-02-07
申请号：US15927949
申请日：2018-03-21
发明人： Hironori OMURA , Hirokazu SANADA , Takako YADA , Ayaka ATSUMI , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20190017085A1
公开(公告)日：2019-01-17
申请号：US16145132
申请日：2018-09-27
发明人： Hironori OMURA , Hirokazu SANADA , Takako Yada , Ayaka Atsumi , Tetsunari MORITA , Emi ISHIMARU
IPC分类号： C12Q1/00 , C12N9/04 , G01N27/327 , C12N9/02 , C12Q1/32
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
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公开(公告)号：US20170226486A1
公开(公告)日：2017-08-10
申请号：US15496935
申请日：2017-04-25
发明人： Takako YADA , Koji MIYAMOTO , Michinari HONDA
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Q1/54 , C12Y101/9901 , G01N27/301 , G01N27/302 , G01N27/3271 , G01N33/66 , G01N2333/902 , G01N2333/904 , Y10T29/49117
摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.
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公开(公告)号：US20170088823A1
公开(公告)日：2017-03-30
申请号：US15126665
申请日：2015-03-19
发明人： Takafumi TAKUMI , Mizuki SHIGESHIRO , Daisuke SATO
CPC分类号： C12N9/0006 , C12N9/96 , C12Q1/32 , C12Q1/54 , C12Y101/9901 , G01N2333/904
摘要： [Problems]To provide a glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition, and a biosensor.[Solutions]A flavin-conjugated glucose dehydrogenase which is composed of proteins having the following amino acid sequence (a), (b) or (c), and having glucose dehydrogenase activity: (a) an amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (b) an amino acid sequence in which one or more amino acids are deleted from, replaced in or added to the amino acid sequence represented by SEQ ID NO: 2, 3, 5, 6, 8 or 9; (c) an amino acid sequence having at least 85% identity with the amino acid sequence represented by SEQ ID NO 2 or 3, at least 95% identity with the amino acid sequence represented by SEQ ID NO 5 or 6, or at least 80% identity with the amino acid sequence represented by SEQ ID NO 8 or 9.
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公开(公告)号：US20150031060A1
公开(公告)日：2015-01-29
申请号：US14510076
申请日：2014-10-08
发明人： Takako YADA , Koji MIYAMOTO , Michinari HONDA
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Q1/54 , C12Y101/9901 , G01N27/301 , G01N27/302 , G01N27/3271 , G01N33/66 , G01N2333/902 , G01N2333/904 , Y10T29/49117
摘要： Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.
摘要翻译： 本文公开了编码FAD-缀合的葡萄糖脱氢酶的基因（多核苷酸），其可以表征为对葡萄糖的反应性，热稳定性，底物识别性能和对麦芽糖的低活性; 使用用该基因转染的转化体细胞来生产酶的方法; 一种测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 以及用于测定葡萄糖的生物传感器。 一个实施方案是编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含其氨基酸序列包含X 1 -X 2 -X 3 -X 4 -X 5 -X 6的多肽，其中X 1和X 2独立地表示脂族氨基酸残基; X3和X6独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基。
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70.发明申请公开(公告)号：US20140234533A1
公开(公告)日：2014-08-21
申请号：US14184573
申请日：2014-02-19
发明人： Hironori Omura , Hirokazu Sanada , Takako Yada , Ayaka Atsumi , Tetsunari Morita , Emi Ishimaru
IPC分类号： C12Q1/00
CPC分类号： C12Q1/006 , C12N9/0004 , C12N9/0006 , C12Q1/004 , C12Q1/32 , C12Y101/05 , C12Y101/9901 , G01N27/3271 , G01N2333/904
摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％以下麦芽糖的活性; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集连接到FAD以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。
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