公开(公告)号：US20170183675A1

公开(公告)日：2017-06-29

申请号：US15449554

申请日：2017-03-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Josef Martin Kuhn ,  Holger Schultheiss ,  Julia Verena Hartig ,  Linda Patricia Loyall ,  Elke Duwenig ,  Marc Saric ,  Maarten Hendrik Stuiver ,  Oliver Oswald

IPC: C12N15/82 ,  A01H4/00

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8223 ,  C12N15/8225 ,  C12N15/8239 ,  C12N15/8241

Abstract: Isolated nucleic acid molecules capable of regulating expression in plants, as well as expression [cassettes, vectors and transgenic plants comprising the same are provided.

公开(公告)号：US20160083743A1

公开(公告)日：2016-03-24

申请号：US14840153

申请日：2015-08-31

Applicant: BASF Plant Science Company GmbH

Inventor： Ana Isabel Sanz Molinero ,  Yves Hatzfeld ,  Valerie Frankard ,  Christophe Reuzeau

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a FSM1-like (Fruit Sant/Myb) polypeptide, or a PIF3-like (PHYTOCHROME INTERACTING FACTOR) polypeptide, or an Uroporphyrinogen III decarboxylase (UROD) polypeptide, or an AS-MTT (Abiotic Stress Membrane Tethered Transcription factor) polypeptide, or an EXO-1 polypeptide, or a YiAP2 (Yield increasing Apetala 2) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a FSM1-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.

Abstract translation: 本发明一般涉及分子生物学领域，涉及一种通过调节植物中编码FSM1样（Fruit Sant / Myb）多肽或PIF3的多核苷酸的核酸表达来增强植物中产量相关性状的方法， 或多肽，或者Ⅳ型多肽，或一种AS-MTT（非生物胁迫膜结合转录因子）多肽，或一种EXO-1多肽或一种YAP2（产量增加的Apetala 2）多肽 。 本发明还涉及具有编码FSM1样多肽的核酸调节表达的植物，该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 本发明还提供了可用于本发明方法的构建体。

公开(公告)号：US20150361440A1

公开(公告)日：2015-12-17

申请号：US14838950

申请日：2015-08-28

Applicant: BASF Plant Science Company GmbH

Inventor： Josef Martin Kuhn ,  Linda Patricia Loyall ,  Malte Siebert ,  Elke Duwenig

IPC: C12N15/82

CPC classification number: C12N15/8234 ,  C12N5/04 ,  C12N15/8216 ,  C12N15/8241

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing seed-specific and/or seed-preferential promoters and the production of plants with enhanced seed-specific and/or seed-preferential expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to the promoters and/or introduced into plants.

Abstract translation: 本发明涉及植物分子生物学领域，并且提供用于生产高表达种子特异性和/或种子优先启动子的方法以及产生具有增强的种子特异性和/或种子优先表达的核酸的植物，其中 核酸表达增强核酸（NEENA）在功能上与启动子连接和/或引入植物。

公开(公告)号：US20150252381A1

公开(公告)日：2015-09-10

申请号：US14417836

申请日：2013-08-01

Applicant: BASF Plant Science Company GmbH

Inventor： Holger Schultheiss ,  Ralf Flachmann

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an HCP4 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP4 protein.

Abstract translation: 本发明涉及一种在植物和/或植物细胞中增加对木瓜属（Pucciniales），优选家蚕科（Phacopsoraceae）的真菌病原体的抗性的方法。 与野生型植物，野生型植物部分和/或野生型植物细胞相比，通过增加植物，植物部分和/或植物细胞中HCP4蛋白或其片段的表达来实现。 此外，本发明涉及具有增加的抗真菌病原体的抗性的转基因植物，植物部分和/或植物细胞，特别是涉及Pucciniales，优选家庭Phacopsoraceae的病原体，以及包含相同序列的重组表达载体 或与编码HCP4蛋白的序列同源。

公开(公告)号：US20150150158A1

公开(公告)日：2015-05-28

申请号：US14353162

申请日：2012-10-19

Applicant: BASF Plant Science Company GmbH ,  Crop Functional Genomic Center

Inventor： Christophe Reuzeau ,  Yang Do Choi ,  Ju Kon Kim ,  Jin Seo Jeong

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

Abstract: A method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid up-regulated upon overexpression of a NAC1 or NAC5-encoding gene, referred to herein as a NUG or NAC up-regulated gene, is provided. Plants having modulated expression of a NUG, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants, are also provided. A method for conferring abiotic stress tolerance in plants, comprising modulating expression of a nucleic acid encoding a NAC1 or NAC5 polypeptide in plants grown under abiotic stress conditions, is also provided. Plants expressing a nucleic acid encoding a NAC1 or NAC5 polypeptide, aside from having increased abiotic stress tolerance, have enhanced yield-related traits and/or modified root architecture compared to corresponding wild type plants. Constructs useful in the methods and plants produced by the methods are also provided.

Abstract translation: 提供了一种通过调节在NAC1或NAC5编码基因（本文中称为NUG或NAC上调基因）过表达上调的核酸的植物中植物表达来增强植物中产量相关性状的方法。 还提供了相对于相应的野生型植物或其他对照植物调节NUG表达的植物，其中植物具有增强的产量相关性状。 还提供了赋予植物非生物胁迫耐受性的方法，其包括在非生物胁迫条件下生长的植物中调节编码NAC1或NAC5多肽的核酸的表达。 除了具有增加的非生物胁迫耐受性之外，表达编码NAC1或NAC5多肽的核酸的植物与相应的野生型植物相比具有增强的产量相关性状和/或修饰的根结构。 还提供了在通过该方法生产的方法和植物中有用的构建体。

公开(公告)号：US20150135365A1

公开(公告)日：2015-05-14

申请号：US14402759

申请日：2013-03-15

Applicant: BASF Plant Science Company GmbH

Inventor： Ana Isabel Sanz Molinero ,  Valerie Frankard

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8271 ,  C07K14/415 ,  C12N15/8261 ,  Y02A40/146

Abstract: A method for enhancing various economically important yield-related traits in plants is provided. More specifically, a method for enhancing one or more yield-related traits in plants is provided, by modulating expression in a plant of a nucleic acid encoding a POI (protein of interest) polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding a POI polypeptide, the plants having one or more enhanced yield-related traits compared with control plants Unknown POI-encoding nucleic acids and constructs comprising the same that useful in performing the methods of the invention are further provided.

Abstract translation: 提供了一种增强植物中各种经济上重要的产量相关性状的方法。 更具体地，通过调节植物中编码POI（目标蛋白）多肽的核酸的表达来提供用于增强植物中一种或多种产量相关性状的方法。 还提供了具有调节表达编码POI多肽的核酸的植物，所述植物具有与对照植物相比具有一个或多个增强的产量相关性状的未知的POI编码核酸和包含其的构建体，其用于实施 进一步提供发明。

公开(公告)号：US20150074842A1

公开(公告)日：2015-03-12

申请号：US14390574

申请日：2013-03-15

Applicant: BASF Plant Science Company GmbH

Inventor： Holger Schultheiss ,  Ralf Flachmann ,  Thomas Subkowski ,  Claus Bollschweiler

IPC: C12N15/82 ,  C07K14/37 ,  A01H1/06 ,  A01H5/00 ,  C12N15/62 ,  A01H1/02

CPC classification number: C12N15/8282 ,  C07K14/37 ,  C07K14/38 ,  C12N15/625

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae plants and/or plant cells. This is achieved for instance by increasing the expression of a hydrophobin protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. In the transgenic plants hydrophobin can be expressed as a fusion protein to facilitate and/or enhance expression. Furthermore, the hydrophobin protein can be expressed including a secretion signal sequence which mediates secretion of the protein into the apoplast and/or into the cuticule.

Abstract translation: 本发明涉及增加对孢子科植物和/或植物细胞的真菌病原体的抗性的方法。 例如通过与野生型植物，野生型植物部分和/或野生型植物细胞相比，增加植物，植物部分和/或植物细胞中的疏水蛋白或其片段的表达来实现。 在转基因植物中，疏水蛋白可以表达为融合蛋白以促进和/或增强表达。 此外，疏水蛋白蛋白质可以被表达，包括介导蛋白质分泌到质外体和/或细胞中的分泌信号序列。

公开(公告)号：US20140325708A1

公开(公告)日：2014-10-30

申请号：US14359867

申请日：2012-11-11

Applicant: BASF Plant Science Company GmbH

Inventor： Steve Vandenabeele

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a PRR-like (Pseudo Response Regulator-like) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a PRR-like polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides an hitherto unknown PRR-like-encoding nucleic acid, and construct comprising the same, useful in performing the methods of the invention.

Abstract translation: 本发明一般涉及分子生物学领域，涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地，本发明涉及通过调节植物中编码PRR样（伪响应调节物样）多肽的核酸表达来增强植物中产量相关性状的方法。 本发明还涉及调节表达编码PRR样多肽的核酸的植物，该植物相对于对照植物具有增强的产量相关性状。 本发明还提供了迄今为止未知的PRR样编码核酸及其构建物，其可用于实施本发明的方法。

公开(公告)号：US12215332B2

公开(公告)日：2025-02-04

申请号：US17413037

申请日：2019-12-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Caspar Langenbach ,  Uwe Conrath ,  Patrick Schwinges ,  Sebastian Beyer ,  Holger Schultheiss ,  David Spencer

IPC: C12N15/82 ,  A61K8/49

Abstract: The present invention pertains to the field of imparting or increasing resistance against fungal pathogens, in particular soy-beanrust, in plants, plant parts, and/or plant cells. This is achieved by increasing the content of scopoletin, scopolin and/or, most preferably, scoparone (6,7-di-methoxycoumarin) in a plant, plant part and/or plant cell. This can in particular be achieved by establishing and/or increasing the expression of an OMT3 enzyme and an F6H1 protein in the respective plant, plant part and/or plant cell; a further way would be to apply a formulation or solution containing scopoletin and/or, most preferably, scoparone to the plant, plant part and/or plant cell. The invention correspondingly also provides proteins, enzymes, expression constructs, plants, plant parts and/or plant cells. In this respect the invention also provides variants of the OMT3 enzyme.

公开(公告)号：US12209108B2

公开(公告)日：2025-01-28

申请号：US16956058

申请日：2018-12-12

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Meghan K. Wyrick ,  Min Xia ,  Janna Slessareva

IPC: C07K1/36 ,  A23J1/00 ,  A23J3/16 ,  C07K1/14

Abstract: The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention, wherein the buffer has a strong alkaline pH of 10 to 12.5 and comprises a soluble concentration of detergent at a level of 0.5% to 5%.