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    Biotic and abiotic stress tolerance in plants
    61.
    发明授权
    Biotic and abiotic stress tolerance in plants 有权
    植物中的生物和非生物胁迫耐受性

    公开(公告)号：US08030546B2

    公开(公告)日：2011-10-04

    申请号：US12077535

    申请日：2008-03-17

    Applicant: T. Lynne Reuber ,  Oliver Ratcliffe ,  Karen S. Century ,  Neal I. Gutterson ,  Roger Canales ,  Emily L. Queen

    Inventor： T. Lynne Reuber ,  Oliver Ratcliffe ,  Karen S. Century ,  Neal I. Gutterson ,  Roger Canales ,  Emily L. Queen

    IPC: A01H5/00 ,  A01H5/10 ,  C12N5/14

    CPC classification number: C07K14/415 ,  C12N15/8261 ,  Y02A40/146

    Abstract: Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

    Abstract translation: 掺入核酸构建体（包括表达载体）的转录因子多核苷酸和多肽已经被引入植物中并被异位表达。 已经显示用许多这些构建体转化的转基因植物对疾病（在一些情况下，多于一种病原体）更耐受，或更耐受非生物胁迫（在某些情况下，对多于一种非生物胁迫）。 非生物胁迫可包括例如盐，高渗胁迫，缺水，热，冷，干旱或低营养条件。

    Disease-inducible promoters
    62.
    发明授权
    Disease-inducible promoters 有权
    疾病诱导型启动子

    公开(公告)号：US07994394B2

    公开(公告)日：2011-08-09

    申请号：US12298134

    申请日：2007-04-23

    Applicant: Luc Adam ,  T. Lynne Reuber ,  Karen S. Century

    Inventor： Luc Adam ,  T. Lynne Reuber ,  Karen S. Century

    IPC: A01H5/00 ,  A01H5/10 ,  C12N15/09 ,  C12N15/82

    CPC classification number: C12Q1/6895 ,  C12N15/8239 ,  C12N15/8282

    Abstract: Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.

    Abstract translation: 已经鉴定了可以用于产生对照植物对疾病具有更高抗性的转基因植物的疾病诱导型启动子序列，并且在外观上是野生型或近似野生型。 这些疾病诱导型启动子中的任何一种可以并入表达载体中，每个包含与启动子可操作地连接的防御反应蛋白。 表达载体可以引入植物，然后外源表达的防御反应蛋白。 已经显示用许多这些表达载体转化的转基因植物对疾病具有更强的抗性，在某些情况下，对一种以上类型的病原体更具抗性，而且在其形态和发育中与野生型植物相似。

    TRANSCRIPTION FACTORS THAT ENHANCE TRAITS IN PLANT ORGANS
    64.
    发明申请
    TRANSCRIPTION FACTORS THAT ENHANCE TRAITS IN PLANT ORGANS 审中-公开
    在工厂机关加强旅行的转录因素

    公开(公告)号：US20100154078A1

    公开(公告)日：2010-06-17

    申请号：US12689010

    申请日：2010-01-18

    Applicant: ANN L. T. POWELL ,  OLIVER J. RATCLIFFE ,  T. LYNNE REUBER ,  ALAN B. BENNETT

    Inventor： ANN L. T. POWELL ,  OLIVER J. RATCLIFFE ,  T. LYNNE REUBER ,  ALAN B. BENNETT

    IPC: A01H5/00 ,  C12N15/82

    CPC classification number: C07K14/415 ,  C12N15/8214 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8267 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8275 ,  C12N15/8282 ,  Y02A40/146

    Abstract: Expression of two Arabidopsis thaliana GARP-family transcription factors, AtGLK1, SEQ ID NO: 2, and AtGLK2, SEQ ID NO: 4, in tomato plants resulted in intensely green fruit that ripen to a normal red color. These Golden2-like (GLK) transcription factors were expressed under the control of several promoters in transgenic tomato lines. When AtGLK1 or AtGLK2 expression was regulated with the constitutive 35S promoter or with three promoters that enhanced expression in fruit tissues, the chlorophyll content of mature green fruit was increased by as much as 100%. The chloroplasts in green fruit expressing AtGLK1 or AtGLK2 developed earlier, were enlarged and had more extensive thylakoid granal development. In addition, expression of AtGLK1 or AtGLK2 resulted in increased starch accumulation in green fruit and higher levels of sugars in ripe fruit. In contrast to wild-type fruit, fruit expressing AtGLK1 developed full green color when they developed in the absence of light. Manipulation of the expression of GLK-like transcription factors in plants may provide a means for improving plant organ nutritional properties, particularly in plants or plant organs grown or maintained under low irradiance.

    Abstract translation: 番茄植物中两种拟南芥GARP家族转录因子AtGLK1，SEQ ID NO：2和AtGLK2，SEQ ID NO：4的表达导致成熟为正常红色的强绿色果实。 这些Golden2样（GLK）转录因子在转基因番茄品系的几种启动子的控制下表达。 当AtGLK1或AtGLK2表达受组成型35S启动子调控时，或3种启动子在果实组织中表达增强时，成熟绿色果实的叶绿素含量增加高达100％。 表达AtGLK1或AtGLK2的绿色果实中的叶绿体早期发育，扩大，并具有更广泛的类囊体发育。 此外，AtGLK1或AtGLK2的表达导致成熟果实中绿色果实中的淀粉积累增加和较高水平的糖。 与野生型水果相比，表达AtGLK1的水果在没有光照时开发出全绿色。 在植物中操纵GLK样转录因子的表达可以提供改善植物器官营养特性的手段，特别是在生长或维持在低辐照度下的植物或植物器官中。

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