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公开(公告)号：US06599407B2
公开(公告)日：2003-07-29
申请号：US09747975
申请日：2000-12-27
申请人： Yuko Taniike , Shin Ikeda , Shiro Nankai
发明人： Yuko Taniike , Shin Ikeda , Shiro Nankai
IPC分类号： G01N27327
CPC分类号： C12Q1/004
摘要： The present invention provides a biosensor having a favorable response characteristic up to a high concentration range, a low blank response and a high storage stability. This sensor comprises an electrode system including a working electrode and a counter electrode, for forming an electrochemical measurement system by coming in contact with a supplied solution; an electrically insulating supporting member for supporting the electrode system; a first reagent layer formed on the working electrode; and a second reagent layer formed on the counter electrode, wherein the first reagent layer comprises an enzyme as the main component, and the second reagent layer comprises an electron mediator as the main component.
摘要翻译： 本发明提供一种生物传感器，其具有高浓度范围，低空白响应和高储存稳定性的良好响应特性。 该传感器包括电极系统，其包括工作电极和对电极，用于通过与所提供的溶液接触形成电化学测量系统; 用于支撑电极系统的电绝缘支撑构件; 形成在工作电极上的第一试剂层; 以及形成在对电极上的第二试剂层，其中第一试剂层包含酶作为主要成分，第二试剂层包含电子介体作为主要成分。
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公开(公告)号：US5171689A
公开(公告)日：1992-12-15
申请号：US339698
申请日：1989-04-18
申请人： Mariko Kawaguri , Shiro Nankai , Takashi Iijima
发明人： Mariko Kawaguri , Shiro Nankai , Takashi Iijima
IPC分类号： C12Q1/00
CPC分类号： C12Q1/001 , Y10S435/817
摘要： As the conventional simple bio-sensors for measuring the particular component in living bodies, there are those in which a change in dye caused by enzyme reaction is detected optically, but such bio-sensors had a problem that precision is low owing to disturbance of the color of liquid samples. With bio-sensors using an enzyme electrode, the precision was high, but operation of measurement was troublesome. The present invention made it possible to detect the concentration of the particular component electromechanically with rapidity, simplicity and high precision by merely putting a porous substrate containing at least enzyme on the electrode system and impregnating the body with a liquid sample.
摘要翻译： 作为用于测量活体中特定成分的传统简单生物传感器，存在其中由酶反应引起的染料变化被光学检测的那些，但是这样的生物传感器具有由于干扰而导致的精度低的问题 液体样品颜色。 使用酶电极的生物传感器，精度高，但测量操作麻烦。 本发明通过仅将至少含有酶的多孔底物放置在电极系统上并用液体样品浸渍身体，可以快速，简便和高精度地机电地检测特定成分的浓度。
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公开(公告)号：US4897173A
公开(公告)日：1990-01-30
申请号：US27204
申请日：1987-02-20
申请人： Shiro Nankai , Mariko Kawaguri , Tashi Iijima
发明人： Shiro Nankai , Mariko Kawaguri , Tashi Iijima
IPC分类号： C12Q1/00 , G01N27/30 , G01N27/416
CPC分类号： C12Q1/004 , Y10S435/817
摘要： This invention relates to a biosensor which comprises an insulative base, an electrode system formed on the substrate and primarily made of carbon, and a perforated body having an enzyme and an electron acceptor and integrally combined with the electrode system whereby a concentration of a specific component in a biological liquid sample can be electrochemically measured rapidly and accurately by a simple procedure of mere addition of the liquid sample. Several simple biosensors for measuring a specific component in a liquid sample are known including a sensor of the type in which a change of a dye formed by an enzyme reaction is optically detected and a sensor of the type using an enzyme electrode. These sensors, respectively, involve an interference with colored matters in liquid sample and necessity of washing of an electrode system every measuring procedure. In addition, the sensors are complicated in structure and materials therefor are expensive. In the present invention, the electrode system is formed mainly of carbon and is integrally combined with the perforated body, so that washing of the electrode system is unnecessary. Thus, an inexpensive, simply-operated biosensor of the disposable type can be provided.
摘要翻译： PCT No.PCT / JP86 / 00311 Sec。 371日期1987年2月20日 102（e）1987年2月20日PCT PCT 1986年6月19日PCT公布。 出版物WO86 / 07632 日本公开日1986年12月31日。本发明涉及一种生物传感器，其包括绝缘基底，形成在基底上并主要由碳制成的电极系统，以及具有酶和电子受体的穿孔体，并与电极系统 从而可以通过简单地加入液体样品的简单程序，快速准确地对生物液体样品中的特定成分的浓度进行电化学测定。 用于测量液体样品中的特定组分的几种简单生物传感器是已知的，包括其中光学检测由酶反应形成的染料变化的类型的传感器和使用酶电极的类型的传感器。 这些传感器分别涉及对液体样品中的有色物质的干扰和每个测量程序洗涤电极系统的必要性。 此外，传感器结构复杂，材料昂贵。 在本发明中，电极系主要由碳形成，与多孔体一体地组合，不需要电极系的清洗。 因此，可以提供廉价的简单操作的一次性型的生物传感器。
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公开(公告)号：US4668594A
公开(公告)日：1987-05-26
申请号：US812889
申请日：1985-12-05
IPC分类号： H01M10/052 , H01M4/485 , H01M10/0566 , H01M4/48 , H01M10/40
CPC分类号： H01M10/052 , H01M4/485
摘要： A rechargeable electrochemical device composed of a negative electrode (4) which comprises an alkali metal as an active material, a non-aqueous electrolyte (6), and a positive electrode (1). The positive electrode (1) is composed of an oxide of chromium and vanadium represented by the general formula:Cr.sub.x V.sub.2(1-x) O.sub.5-(2+y)x(wherein 0.2.ltoreq.x.ltoreq.0.9, 0.1.ltoreq.y.ltoreq.1.0). The rechargeable electrochemical device offers a high discharge voltage, a large discharge capacity, linear discharge voltage, and the capacity to withstand over-charging.
摘要翻译： PCT No.PCT / JP85 / 00162 Sec。 371日期1985年12月5日第 102（e）1985年12月5日日期PCT提交1985年4月3日PCT公布。 公开号WO85 / 04763 日期：1985年10月24日。一种由负极（4）构成的可充电电化学装置，其包含作为活性物质的碱金属，非水电解质（6）和正极（1）。 正极（1）由以下通式表示的铬和钒的氧化物构成：CrxV2（1-x）O5-（2 + y）x（其中0.2
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公开(公告)号：US4224125A
公开(公告)日：1980-09-23
申请号：US946527
申请日：1978-09-26
IPC分类号： H01M4/90 , C12Q1/00 , G01N27/327 , H01M8/16 , G01N31/14
CPC分类号： H01M4/9008 , C12Q1/004 , H01M8/16 , Y02E60/527 , Y10S435/817
摘要： An enzyme electrode, which comprises an oxidoreductase including, where necessary, a coenzyme; a redox polymer acting as an electron mediator in an enzymatic reaction; and an electron collector, wherein the oxidoreductase and the redox polymer are both maintained in the immobilized state on or in the neighborhood of the electron collector by either one of the methods of; entrapping them with a semi-permeable membrane in the neighborhood of the electron collector, or chemically superimposing them on the electron collector or moulding them including the electron collector in a unit with the help of chemical treatment.
摘要翻译： 一种酶电极，其包含氧化还原酶，其在必要时包括辅酶; 在酶反应中作为电子介体的氧化还原聚合物; 和电子收集器，其中所述氧化还原酶和所述氧化还原聚合物在所述电子收集器的附近或附近都被保持在固定状态。 在电子收集器附近用半透膜截留它们，或者在化学处理的帮助下将它们化学地叠加在电子收集器上或者将它们包括在一个单元中的电子收集器中。
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公开(公告)号：US5185256A
公开(公告)日：1993-02-09
申请号：US774129
申请日：1991-10-15
申请人： Shiro Nankai , Mariko Kawaguri , Takashi Iijima
发明人： Shiro Nankai , Mariko Kawaguri , Takashi Iijima
CPC分类号： C12Q1/004 , G01N27/3272 , Y10S435/817
摘要： This invention relates to a biosensor which comprises an insulative base, an electrode system formed on the substrate and primarily made of carbon, and a perforated body having an enzyme and an electron acceptor and integrally combined with the electrode system whereby a concentration of a specific component in a biological liquid sample can be electrochemically measured rapidly and accurately by a simple procedure of mere addition of the liquid sample. Several simple biosensors for measuring a specific component in a liquid sample are known including a sensor of the type in which a change of a dye formed by an enzyme reaction is optically detected and a sensor of the type using an enzyme electrode. These sensors, respectively, involve an interference with colored matters in liquid sample and necessity of washing of an electrode system every measuring procedure. In addition, the sensors are complicated in structure and materials therefor are expensive. In the present invention, the electrode system is formed mainly of carbon and is integrally combined with the perforated body, so that washing of the electrode system is unnecessary. Thus, an inexpensive, simply-operated biosensor of the disposable type can be provided.
摘要翻译： 本发明涉及一种生物传感器，其包括绝缘基底，形成在基底上并主要由碳制成的电极系统，以及具有酶和电子受体的穿孔体，与电极系统整体结合，由此使特定成分 在生物液体样品中，可以通过仅添加液体样品的简单方法快速且准确地进行电化学测量。 用于测量液体样品中的特定组分的几种简单的生物传感器是已知的，包括其中光学检测由酶反应形成的染料变化的类型的传感器和使用酶电极的类型的传感器。 这些传感器分别涉及对液体样品中的有色物质的干扰和每个测量程序洗涤电极系统的必要性。 此外，传感器结构复杂，材料昂贵。 在本发明中，电极系主要由碳形成，与多孔体一体地组合，不需要电极系的清洗。 因此，可以提供廉价的简单操作的一次性型的生物传感器。
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公开(公告)号：US5120420A
公开(公告)日：1992-06-09
申请号：US445632
申请日：1989-11-27
申请人： Shiro Nankai , Mariko Kawaguri , Mayumi Ohtani , Takashi Iijima
发明人： Shiro Nankai , Mariko Kawaguri , Mayumi Ohtani , Takashi Iijima
IPC分类号： C12Q1/00 , G01N27/30 , G01N33/487
CPC分类号： G01N27/3272 , C12Q1/001 , C12Q1/004 , Y10S435/817 , Y10S436/806
摘要： A biosensor of the invention comprises an insulating base board (1) having formed thereon, in sequence, leads (2, 3, 3'), an electrode system mainly made of carbon (4, 5, 5'), an insulating layer (6) and a reaction layer (14) composed of an enzyme and an electron acceptor, and being provided thereon with a space (8) defined by a spacer (7) and a cover 9). When a biological sample solution is brought into contact with the inlet (10) of the biosensor having the above-described structure, the sample solution is introduced into its inside, while the air within the space (8) is rapidly discharged through the outlet (11) and, at the same time, the space (8) is filled with the sample solution up to the neighborhood of the outlet. Thus, measurement can be conducted inexpensively at a high speed with a high accuracy through simple procedures without residual bubbles.
摘要翻译： PCT No.PCT / JP89 / 00337 Sec。 371日期1990年1月31日第 102（e）1990年1月31日PCT PCT 1989年3月31日PCT。 出版物WO89 / 09397 日本特开1989年1月5日。本发明的生物传感器包括：绝缘基板（1），其上依次形成引线（2,3,3'），主要由碳（4,5,5 '），由酶和电子受体构成的绝缘层（6）和反应层（14），并且在其上设置有由间隔物（7）和盖9限定的空间（8）。 当生物样品溶液与具有上述结构的生物传感器的入口（10）接触时，将样品溶液引入其内部，同时空间（8）内的空气通过出口（ 11），并且同时，空间（8）被填充有样品溶液直到出口附近。 因此，通过没有残留气泡的简单程序，可以高精度地以高精度进行测量。
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公开(公告)号：US4431507A
公开(公告)日：1984-02-14
申请号：US338957
申请日：1982-01-12
申请人： Shiro Nankai , Akihiro Imai , Takashi Iijima
发明人： Shiro Nankai , Akihiro Imai , Takashi Iijima
IPC分类号： G01N27/327 , C12Q1/00 , G01N27/54 , C12Q1/54
CPC分类号： C12Q1/001 , Y10S435/817
摘要： The disclosure is directed to an improved enzyme electrode which includes a first electrode having at least one kind of an enzyme immobilized on it for electrochemically detecting a substance to be produced in association with a reaction based on the enzyme, and a second electrode for electrochemically removing materials which interfere with the detection by the first electrode. The second electrode is disposed at the side of a test solution containing a substrate of the enzyme with respect to the first electrode.
摘要翻译： 本发明涉及一种改进的酶电极，其包括第一电极，其具有固定在其上的至少一种酶，用于电化学检测与基于酶的反应相关的待产生物质，以及用于电化学去除的第二电极 干扰第一电极检测的材料。 第二电极设置在包含酶底物相对于第一电极的测试溶液的一侧。
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公开(公告)号：US4392933A
公开(公告)日：1983-07-12
申请号：US358609
申请日：1982-03-15
申请人： Kenichi Nakamura , Shiro Nankai , Takashi Iijima
发明人： Kenichi Nakamura , Shiro Nankai , Takashi Iijima
IPC分类号： G01N27/327 , C12Q1/00 , C12Q1/26
CPC分类号： C12Q1/005 , Y10S435/817
摘要： An immobilized enzyme electrode effective in measurement of the substrate concentration of the enzyme and in conversion from enzyme reaction energies into electric energies. The immobilized enzyme, of an oxidase system, such as glucose oxidase, amino acid oxidase, xanthine oxidase or the like and a metal oxide capable of constituting a redox system which is reduced through coupling with these enzyme reactions and is electrochemically oxidized (anodic oxidation) are combined with each other. The use of the enzyme electrode allows the determination quantity of the enzyme substrate as extremely low as approximately 10.sup.-5 to 10.sup.-6 mole/l in concentration.
摘要翻译： 固定化酶电极，有效测量酶的底物浓度，并从酶反应能转化为电能。 氧化酶系统如葡萄糖氧化酶，氨基酸氧化酶，黄嘌呤氧化酶等的固定化酶和能够构成氧化还原体系的金属氧化物，其通过与这些酶反应的偶联而还原，并进行电化学氧化（阳极氧化） 相互结合。 使用酶电极使得酶底物的测定量极低至约10-5至10-6摩尔/升浓度。
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公开(公告)号：US4376689A
公开(公告)日：1983-03-15
申请号：US321326
申请日：1981-11-13
申请人： Kenichi Nakamura , Shiro Nankai , Takashi Iijima
发明人： Kenichi Nakamura , Shiro Nankai , Takashi Iijima
CPC分类号： C12Q1/005 , Y10S435/817
摘要： Improvements in a coenzyme electrode used to electrochemically measure the activity of enzyme or substrate concentration of the enzyme easily. The coenzyme is immobilized, without using a semipermeable membrane, on the electron collector directly with the chemical bond, particularly the covalent bond, whereby the activity of the oxide-reductase requiring the coenzyme can be measured. In addition, the oxide-reductase requiring the coenzyme is also immobilized together with such immobilized coenzyme to improve the characteristics of the conventional enzyme-coenzyme immobilized electrode.
摘要翻译： 用于电化学测量酶活性或酶底物浓度的辅酶电极的改进容易。 辅酶不用半透膜即可直接用化学键，特别是共价键直接固定在电子收集器上，从而可以测量需要辅酶的氧化还原酶的活性。 此外，需要辅酶的氧化还原酶也与这样的固定化辅酶一起固定，以改善常规的酶 - 辅酶固定化电极的特性。
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