公开(公告)号：US4897173A

公开(公告)日：1990-01-30

申请号：US27204

申请日：1987-02-20

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Tashi Iijima

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Tashi Iijima

IPC分类号： C12Q1/00 ,  G01N27/30 ,  G01N27/416

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： This invention relates to a biosensor which comprises an insulative base, an electrode system formed on the substrate and primarily made of carbon, and a perforated body having an enzyme and an electron acceptor and integrally combined with the electrode system whereby a concentration of a specific component in a biological liquid sample can be electrochemically measured rapidly and accurately by a simple procedure of mere addition of the liquid sample. Several simple biosensors for measuring a specific component in a liquid sample are known including a sensor of the type in which a change of a dye formed by an enzyme reaction is optically detected and a sensor of the type using an enzyme electrode. These sensors, respectively, involve an interference with colored matters in liquid sample and necessity of washing of an electrode system every measuring procedure. In addition, the sensors are complicated in structure and materials therefor are expensive. In the present invention, the electrode system is formed mainly of carbon and is integrally combined with the perforated body, so that washing of the electrode system is unnecessary. Thus, an inexpensive, simply-operated biosensor of the disposable type can be provided.

摘要翻译： PCT No.PCT / JP86 / 00311 Sec。 371日期1987年2月20日 102（e）1987年2月20日PCT PCT 1986年6月19日PCT公布。 出版物WO86 / 07632 日本公开日1986年12月31日。本发明涉及一种生物传感器，其包括绝缘基底，形成在基底上并主要由碳制成的电极系统，以及具有酶和电子受体的穿孔体，并与电极系统 从而可以通过简单地加入液体样品的简单程序，快速准确地对生物液体样品中的特定成分的浓度进行电化学测定。 用于测量液体样品中的特定组分的几种简单生物传感器是已知的，包括其中光学检测由酶反应形成的染料变化的类型的传感器和使用酶电极的类型的传感器。 这些传感器分别涉及对液体样品中的有色物质的干扰和每个测量程序洗涤电极系统的必要性。 此外，传感器结构复杂，材料昂贵。 在本发明中，电极系主要由碳形成，与多孔体一体地组合，不需要电极系的清洗。 因此，可以提供廉价的简单操作的一次性型的生物传感器。

公开(公告)号：US5320732A

公开(公告)日：1994-06-14

申请号：US75859

申请日：1993-06-11

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Minoru Fukuda

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Minoru Fukuda

IPC分类号： G01N27/28 ,  G01N33/487 ,  G01N27/26

CPC分类号： G01N27/3272

摘要： The subject invention provides a simple sensor and an easy to handle biosensor measuring apparatus for the purpose of making measurements easily, rapidly and with high accuracy for specific components within a biological specimen such as blood.The biosensor of the subject invention is provided with a protrusion or a depression in a portion of a base having at least a measurement electrode and an opposing electrode. Also, the biosensor measuring apparatus is so configurated to have a mating means in the main body of the measuring apparatus freely supporting this biosensor to contact the said sensor's protrusion or depression, and further having the activating switch of the driving power supply located in this mating means.By means of these, it is possible to prevent wasteful measuring operations, as noted in the prior art biosensors, such as inserting the sensor backwards and making measurements with the sensor inserted backwards.

摘要翻译： 本发明提供了一种简单的传感器和易于处理的生物传感器测量装置，用于容易，快速且高度精确地测量诸如血液的生物样本内的特定组分的目的。 本发明的生物传感器在至少具有测量电极和相对电极的基部的一部分中设置有突起或凹陷。 此外，生物传感器测量装置被配置为具有在测量装置的主体中具有自由支撑该生物传感器的配合装置以接触所述传感器的突起或凹陷，并且还具有位于该配合中的驱动电源的启动开关 手段。 通过这些，可以防止浪费的测量操作，如现有技术的生物传感器中所述，例如向后插入传感器并且使传感器向后插入进行测量。

公开(公告)号：US5171689A

公开(公告)日：1992-12-15

申请号：US339698

申请日：1989-04-18

申请人： Mariko Kawaguri ,  Shiro Nankai ,  Takashi Iijima

发明人： Mariko Kawaguri ,  Shiro Nankai ,  Takashi Iijima

IPC分类号： C12Q1/00

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： As the conventional simple bio-sensors for measuring the particular component in living bodies, there are those in which a change in dye caused by enzyme reaction is detected optically, but such bio-sensors had a problem that precision is low owing to disturbance of the color of liquid samples. With bio-sensors using an enzyme electrode, the precision was high, but operation of measurement was troublesome. The present invention made it possible to detect the concentration of the particular component electromechanically with rapidity, simplicity and high precision by merely putting a porous substrate containing at least enzyme on the electrode system and impregnating the body with a liquid sample.

摘要翻译： 作为用于测量活体中特定成分的传统简单生物传感器，存在其中由酶反应引起的染料变化被光学检测的那些，但是这样的生物传感器具有由于干扰而导致的精度低的问题 液体样品颜色。 使用酶电极的生物传感器，精度高，但测量操作麻烦。 本发明通过仅将至少含有酶的多孔底物放置在电极系统上并用液体样品浸渍身体，可以快速，简便和高精度地机电地检测特定成分的浓度。

公开(公告)号：US5266179A

公开(公告)日：1993-11-30

申请号：US733130

申请日：1991-07-19

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

IPC分类号： G01N27/26 ,  G01N27/28 ,  G01N27/416 ,  G01N27/49

CPC分类号： G01N33/48771 ,  G01N2035/009

摘要： A sensor 13 is inserted into a connector 14. A constant voltage required to obtain a response current is applied across the connector 14 by a voltage applying source 15 at timings required. A response current of the sensor 13 inserted into the connector 14 is converted into a voltage by a current-to-voltage converter 16, and the amount thereof is determined by a microcomputer, the analysis results being displayed onto a display unit.

公开(公告)号：US5229282A

公开(公告)日：1993-07-20

申请号：US617886

申请日：1990-11-26

申请人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai

IPC分类号： C12Q1/00

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The present invention relates to a process of preparation of a biosensor comprising forming an electrode system mainly containing carbon on an insulating base plate, treating the surface of electrode system with an organic solvent, and then arranging a reaction layer on the electrode system to give a unified element. The reaction layer contains an enzyme, electron acceptor and a hydrophilic polymer. Treatment with the organic solvent improves adhesion of the reaction layer to the electrode system. The electrode system contains a working electrode and a counter electrode. The electrode system is formed from a carbon paste containing a resin binder. Treatment is preferably carried out by wiping the surface of the electrodes with a material impregnated by the organic solvent.

公开(公告)号：US5512159A

公开(公告)日：1996-04-30

申请号：US293131

申请日：1994-08-19

申请人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

发明人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

IPC分类号： G01N27/28 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/00 ,  C12Q1/54 ,  G01N27/327 ,  G01N33/483

CPC分类号： C12Q1/001

摘要： A biosensor of the present invention is provided with an insulating base plate, an electrode system mainly consisting of a working electrode and a counter electrode formed on the insulating base plate, and a reaction layer on the electrode system. The counter electrode is partially a circular arc. A manufacturing method of the biosensor of the present invention is comprised of a step to form a base by arranging leads, an electrode system, and an insulating layer on an insulating base plate, and a step to form a reaction layer mainly composed of an enzyme on the electrode system. Since the counter electrode is partially in the shape of a circular arc, the reaction layer is easily formed and prevented from delaminating, whereby the measuring accuracy, preservative properties and reliability of the biosensor are improved. The present invention enables highly efficient biosensors to be mass produced at low cost.

摘要翻译： 本发明的生物传感器设置有绝缘基板，主要由在绝缘基板上形成的工作电极和对电极组成的电极系统和电极系统上的反应层。 对电极部分是圆弧。 本发明的生物传感器的制造方法包括通过在绝缘基板上排列引线，电极系统和绝缘层形成基底的步骤，以及形成主要由酶组成的反应层的步骤 在电极系统上。 由于对电极部分为圆弧形状，反应层易于形成并防止分层，从而提高了生物传感器的测量精度，防腐性能和可靠性。 本发明能够以低成本批量生产高效生物传感器。

公开(公告)号：US5185256A

公开(公告)日：1993-02-09

申请号：US774129

申请日：1991-10-15

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Takashi Iijima

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Takashi Iijima

IPC分类号： C12Q1/00 ,  G01N27/30

CPC分类号： C12Q1/004 ,  G01N27/3272 ,  Y10S435/817

摘要： This invention relates to a biosensor which comprises an insulative base, an electrode system formed on the substrate and primarily made of carbon, and a perforated body having an enzyme and an electron acceptor and integrally combined with the electrode system whereby a concentration of a specific component in a biological liquid sample can be electrochemically measured rapidly and accurately by a simple procedure of mere addition of the liquid sample. Several simple biosensors for measuring a specific component in a liquid sample are known including a sensor of the type in which a change of a dye formed by an enzyme reaction is optically detected and a sensor of the type using an enzyme electrode. These sensors, respectively, involve an interference with colored matters in liquid sample and necessity of washing of an electrode system every measuring procedure. In addition, the sensors are complicated in structure and materials therefor are expensive. In the present invention, the electrode system is formed mainly of carbon and is integrally combined with the perforated body, so that washing of the electrode system is unnecessary. Thus, an inexpensive, simply-operated biosensor of the disposable type can be provided.

摘要翻译： 本发明涉及一种生物传感器，其包括绝缘基底，形成在基底上并主要由碳制成的电极系统，以及具有酶和电子受体的穿孔体，与电极系统整体结合，由此使特定成分 在生物液体样品中，可以通过仅添加液体样品的简单方法快速且准确地进行电化学测量。 用于测量液体样品中的特定组分的几种简单的生物传感器是已知的，包括其中光学检测由酶反应形成的染料变化的类型的传感器和使用酶电极的类型的传感器。 这些传感器分别涉及对液体样品中的有色物质的干扰和每个测量程序洗涤电极系统的必要性。 此外，传感器结构复杂，材料昂贵。 在本发明中，电极系主要由碳形成，与多孔体一体地组合，不需要电极系的清洗。 因此，可以提供廉价的简单操作的一次性型的生物传感器。

公开(公告)号：US5120420A

公开(公告)日：1992-06-09

申请号：US445632

申请日：1989-11-27

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Mayumi Ohtani ,  Takashi Iijima

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Mayumi Ohtani ,  Takashi Iijima

IPC分类号： C12Q1/00 ,  G01N27/30 ,  G01N33/487

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/004 ,  Y10S435/817 ,  Y10S436/806

摘要： A biosensor of the invention comprises an insulating base board (1) having formed thereon, in sequence, leads (2, 3, 3'), an electrode system mainly made of carbon (4, 5, 5'), an insulating layer (6) and a reaction layer (14) composed of an enzyme and an electron acceptor, and being provided thereon with a space (8) defined by a spacer (7) and a cover 9). When a biological sample solution is brought into contact with the inlet (10) of the biosensor having the above-described structure, the sample solution is introduced into its inside, while the air within the space (8) is rapidly discharged through the outlet (11) and, at the same time, the space (8) is filled with the sample solution up to the neighborhood of the outlet. Thus, measurement can be conducted inexpensively at a high speed with a high accuracy through simple procedures without residual bubbles.

摘要翻译： PCT No.PCT / JP89 / 00337 Sec。 371日期1990年1月31日第 102（e）1990年1月31日PCT PCT 1989年3月31日PCT。 出版物WO89 / 09397 日本特开1989年1月5日。本发明的生物传感器包括：绝缘基板（1），其上依次形成引线（2,3,3'），主要由碳（4,5,5 '），由酶和电子受体构成的绝缘层（6）和反应层（14），并且在其上设置有由间隔物（7）和盖9限定的空间（8）。 当生物样品溶液与具有上述结构的生物传感器的入口（10）接触时，将样品溶液引入其内部，同时空间（8）内的空气通过出口（ 11），并且同时，空间（8）被填充有样品溶液直到出口附近。 因此，通过没有残留气泡的简单程序，可以高精度地以高精度进行测量。

公开(公告)号：US06452653B1

公开(公告)日：2002-09-17

申请号：US09619655

申请日：2000-07-19

申请人： Yasuhiko Yamanaka ,  Naohide Wakita ,  Takeshi Karasawa ,  Mariko Kawaguri ,  Seiji Nishiyama

发明人： Yasuhiko Yamanaka ,  Naohide Wakita ,  Takeshi Karasawa ,  Mariko Kawaguri ,  Seiji Nishiyama

IPC分类号： G02F11335

CPC分类号： G02F1/133553 ,  G02F1/136227 ,  G02F2001/136236

摘要： A reflector comprises a substrate provided with a plurality of projecting and depressed structures which are groups of columnar portions each serving as a basic unit and composed of a plurality of minute columnar portions, which are separate from each other or at least partially connected to each other, and with a light reflecting thin film provided over the projecting and depressed structures. This allows incident light to be scattered and reflected in a forward direction or the like, not in the regular reflection direction, and provides a reflector having a superior contrast property and a superior paper-white property, a fabrication method therefor, and a reflective display device comprising the reflector. The reflective display device has a liquid crystal layer provided between a pair of substrates. One of the pair of substrates is provided with projecting and depressed portions covered with a metal film and with a support portion for supporting the counter substrate, which are molded integrally. The arrangement reduces reflection in the regular reflection direction and enhances brightness and whiteness, while reducing the occurrence of uneven display by providing a uniform cell gap.

摘要翻译： 反射器包括设置有多个突出和凹陷结构的基板，该多个突出和凹陷结构是每个用作基本单元并且由彼此分离或至少部分地彼此连接的多个微小柱状部分组成的柱状部分 并且具有设置在突出和凹陷结构上的光反射薄膜。 这允许入射光在正向反射方向上以向前方向等散射和反射，并且提供具有优异的对比度特性和优异的纸白色特性的反射器，其制造方法和反射显示 装置包括反射器。 反射型显示装置具有设置在一对基板之间的液晶层。 一对基板中的一个设置有被金属膜覆盖的突出和凹陷部分以及用于支撑相对基板的支撑部分，该支撑部分被一体模制。 通过提供均匀的单元间隙，能够减少正反射方向的反射，提高亮度和白度，同时减少不均匀显示的发生。

公开(公告)号：US07495821B2

公开(公告)日：2009-02-24

申请号：US10695669

申请日：2003-10-29

申请人： Hiroyuki Yamakita ,  Toshiyasu Oue ,  Naohide Wakita ,  Mariko Kawaguri

发明人： Hiroyuki Yamakita ,  Toshiyasu Oue ,  Naohide Wakita ,  Mariko Kawaguri

IPC分类号： G02B26/00

CPC分类号： G02F1/167 ,  G02F2001/1678 ,  G02F2201/12

摘要： A thin display device is provided which is capable of displaying images at a lowered driving voltage in a shortened response time by causing fine particles to travel in a gaseous phase. The display device includes: a display section having upper substrate 1 and lower substrate 2 each having a thickness ranging from about 0.1 mm to about 0.5 mm, which are disposed opposite to each other; colored particles 6 having a particle diameter ranging from about 1 μm to about 10 μm packed in an air layer 7 provided in the gap between the upper substrate 1 and the lower substrate 2; and first electrode 3 and second electrode 4 formed on the underside of the upper substrate 1. The colored particles 6 used in the display device are electrostatically charged either negatively or positively and are caused to travel between the first electrode 3 and the second electrode 4 in accordance with voltage applied to the first and second electrodes 3 and 4.

摘要翻译： 提供了一种薄型显示装置，其能够通过使细颗粒在气相中行进，以缩短的响应时间以较低的驱动电压显示图像。 显示装置包括：显示部，其具有彼此相对设置的厚度范围为约0.1mm至约0.5mm的上基板1和下基板2; 填充在设置在上基板1和下基板2之间的间隙中的空气层7中的粒径范围为约1μm至约10μm的着色颗粒6; 以及形成在上基板1的下侧上的第一电极3和第二电极4.在显示装置中使用的着色颗粒6被负电或积极地静电充电，并使其在第一电极3和第二电极4之间行进 根据施加到第一和第二电极3和4的电压。