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    Modified DNA Cleavage and Methods for Use
    62.
    发明申请
    Modified DNA Cleavage and Methods for Use 有权
    修饰的DNA切割和使用方法

    公开(公告)号：US20110065182A1

    公开(公告)日：2011-03-17

    申请号：US12949182

    申请日：2010-11-18

    Applicant: Chudi Guan ,  Sanjay Kumar ,  Rebecca Kucera

    Inventor： Chudi Guan ,  Sanjay Kumar ,  Rebecca Kucera

    IPC: C12N5/10 ,  C07H21/04 ,  C12N15/63

    CPC classification number: C12N9/22

    Abstract: Compositions and methods are provided that relate to a modified DNA cleaving enzyme having at least 35% amino acid sequence identity with T7 Endo I. The modified enzyme includes two catalytic centers separated by a β-bridge where the β-bridge contains at least one mutation having an effect of altering enzyme cleavage activity compared to the unmodified enzyme. Activities associated with the modified DNA cleaving enzyme that can be modulated in different reaction conditions include at least one of: (a) non-sequence specific nicking activity; (b) cleaving the second strand of a duplex DNA at a preexisting nick site to produce a linear duplex with a single strand overhang; (c) non-sequence specific DNA cleavage; (d) cleaving DNA flanking a mismatch; and (e) cleavage at a cruciform structure in a DNA duplex.

    Abstract translation: 提供了与T7 Endo I具有至少35％的氨基酸序列同一性的修饰的DNA切割酶的组合物和方法。修饰的酶包括两个由“桥”分开的催化中心，其中“桥”至少包含 一个突变与未修饰的酶相比具有改变酶裂解活性的作用。 与可以在不同反应条件下调节的修饰的DNA切割酶相关的活性包括以下至少一个：（a）非序列特异性切口活性; （b）在预先存在的切口位点切割双链体DNA的第二链以产生具有单链突出端的线性双链体; （c）非序列特异性DNA切割; （d）切断位于不对称侧翼的DNA; 和（e）在DNA双链体中的十字形结构处的切割。

    Method and system for managing bandwidth in a virtualized system
    64.
    发明授权
    Method and system for managing bandwidth in a virtualized system 有权
    用于管理虚拟化系统中的带宽的方法和系统

    公开(公告)号：US07774794B2

    公开(公告)日：2010-08-10

    申请号：US11207544

    申请日：2005-08-19

    Applicant: Kiran Panesar ,  Michael Goldsmith ,  Sanjay Kumar ,  Philip Lantz

    Inventor： Kiran Panesar ,  Michael Goldsmith ,  Sanjay Kumar ,  Philip Lantz

    IPC: G06F9/44 ,  G06F13/00 ,  G06F9/455 ,  G06F9/46 ,  G06F3/00 ,  G06F5/00

    CPC classification number: G06F13/105 ,  G06F2213/0042

    Abstract: A method of improving USB device virtualization to prevent bus bandwidth from being over allocated when isochronous USB devices are attached to multiple virtual machines by attaching a dummy device to each virtual machine which will mimic the bandwidth reservations made by real devices in other virtual machines, thus allowing each virtual machine to determine the true available bandwidth. The dummy devices are represented by incorporating a dummy device driver in each virtual machine and emulating the dummy device in software in the VMM.

    Abstract translation: 一种改进USB设备虚拟化的方法，以通过将虚拟设备附加到每个虚拟机上来同步同步USB设备连接到多个虚拟机，以防止总线带宽过度分配，这将虚拟虚拟机中的实际设备所做的带宽预留，从而 允许每个虚拟机确定真实的可用带宽。 虚拟设备通过在每个虚拟机中并入虚拟设备驱动器并以VMM中的软件模拟虚拟设备来表示。

    Process for the production of Portland slag cement using granulated blast furnace slag
    67.
    发明授权
    Process for the production of Portland slag cement using granulated blast furnace slag 失效
    使用造粒高炉矿渣生产波特兰矿渣水泥的工艺

    公开(公告)号：US07410537B2

    公开(公告)日：2008-08-12

    申请号：US11491916

    申请日：2006-07-25

    Applicant: Rakesh Kumar ,  Sanjay Kumar ,  Amitava Bandopadhaya ,  Surya Pratap Mehrotra

    Inventor： Rakesh Kumar ,  Sanjay Kumar ,  Amitava Bandopadhaya ,  Surya Pratap Mehrotra

    IPC: C04B7/04

    CPC classification number: C04B28/04 ,  C04B18/141 ,  Y02W30/94 ,  C04B20/008 ,  C04B20/026

    Abstract: Accordingly, the present invention provides an improved process for the production of Portland slag cement using granulated blast furnace slag, which comprises: (viii) forming of cement clinker by known process, (ix) ball-milling of cement clinker for a period ranging between 30-60 minutes in dry condition, (x) reducing size of granulated blast furnace slag by any process to obtain the size in the range between 210 to 100 μm for using as feed for attrition mill, (xi) wet milling of granulated blast furnace slag for a period ranging between 5-15 minutes in an attrition mill using granulated blast furnace slag to water ratio in the range of 1:1 to 1:2 and granulated blast furnace slag to grinding bail ratio in the range of 1:5 to 1:15, (xii) removing water from the slurry obtained after attrition milling by known process, (xiii) drying of the obtained slurry by known process, (xiv) mixing intimately of: attrition milled slag obtain in step (vi) in the range of: 50 to 95% by weight ball-milled clinker obtain in step (ii) in the range of: 05 to 45% by weight gypsum in the range of: 01 to 05% by weight for a period in the range of 15 to 30 minutes.

    Abstract translation: 因此，本发明提供了一种使用造粒高炉矿渣生产波特兰矿渣水泥的改进方法，其包括：（viii）通过已知方法形成水泥熟料，（ix）水泥熟料的球磨，时间范围 在干燥条件下30-60分钟，（x）通过任何工艺减少造粒高炉矿渣的尺寸，以获得用于磨粉机进料的210至100um之间的尺寸，（xi）造粒高炉的湿磨 在磨粉机中使用造粒高炉矿渣与水的比例在1：1至1：2范围内的炉渣在5-15分钟之间，并将造粒高炉矿渣与1：5至 （xii）通过已知方法磨碎后得到的浆料中的水，（xiii）通过已知方法干燥所获得的浆料，（xiv）密切混合：步骤（vi）中获得的磨碎的炉渣 范围：50〜95重量％ 研磨熟料在步骤（ii）中获得的范围为：05至45重量％的石膏，其范围为：01至05重量％，范围为15至30分钟。

    Cloning of novel gene sequences expressed and repressed during winter dormancy in the apical buds of tea
    70.
    发明申请
    Cloning of novel gene sequences expressed and repressed during winter dormancy in the apical buds of tea 审中-公开
    克隆在冬季休眠期间在茶叶顶端表达和抑制的新基因序列

    公开(公告)号：US20070011780A1

    公开(公告)日：2007-01-11

    申请号：US11516532

    申请日：2006-09-06

    Applicant: Sanjay Kumar ,  Lakhvir Lal ,  Paramvir Ahuja

    Inventor： Sanjay Kumar ,  Lakhvir Lal ,  Paramvir Ahuja

    IPC: A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N5/04 ,  C07K14/415

    CPC classification number: C07K14/415

    Abstract: The present invention relates to cloning of novel gene sequences expressed and repressed during winter dormancy in the apical buds of Camellia sinesis L. (O.) Kuntze (hereinafter, referred to tea) bush, particularly, relates to identification, cloning and analysis of novel 3 prime (hereinafter called as 3′) ends of the genes (gene in the present invention refers to the deoxyribonucleic acid (hereinafter known as, DNA) sequences that are expressed and repressed in winter-dormant apical buds of tea. 3′ end refers to that end of DNA which has free hydroxyl group at 3rd position of the carbohydrate moiety of the DNA molecule.

    Abstract translation: 本发明涉及在冬季休眠期间在山茶花（O.）Kuntze（以下称为茶）灌木的顶芽中表达和抑制的新基因序列的克隆，特别涉及新颖的鉴定，克隆和分析 3个基因（以下称为3'）末端（本发明中的基因是指在冬季休眠的顶端芽中表达和抑制的脱氧核糖核酸（以下称为DNA）序列，3'末端是指 到DNA分子的碳水化合物部分的第3位具有游离羟基的DNA的末端。

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