公开(公告)号：US06713065B2

公开(公告)日：2004-03-30

申请号：US09021660

申请日：1998-02-10

Applicant: Margaret H. Baron ,  Sarah M. Farrington ,  Maria Belaoussoff

Inventor： Margaret H. Baron ,  Sarah M. Farrington ,  Maria Belaoussoff

IPC: A61K3818

CPC classification number: G01N33/5073 ,  A01K67/0275 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0331 ,  A01K2267/0381 ,  A61K38/1703 ,  A61K38/1875 ,  C12N5/0641 ,  C12N5/0647 ,  C12N5/069 ,  C12N15/8509 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2503/02 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5041 ,  G01N33/5094 ,  A61K2300/00

Abstract: This application pertains to methods and compositions that modulate proliferation and/or differentiation of undifferentiated mesodermally-derived cells so as to have an effect on at least one of vascular growth and hematopoiesis.

Abstract translation: 本申请涉及调节未分化的中胚层衍生细胞的增殖和/或分化以对血管生长和造血作用至少一种的作用的方法和组合物。

公开(公告)号：US06677307B2

公开(公告)日：2004-01-13

申请号：US10138158

申请日：2002-05-01

Applicant: Daniel R. Twardzik ,  Andre Pernet ,  Thomas S. Felker ,  Stefan Paskell ,  John M. Reno

Inventor： Daniel R. Twardzik ,  Andre Pernet ,  Thomas S. Felker ,  Stefan Paskell ,  John M. Reno

IPC: A61K3818

CPC classification number: C07K14/495 ,  A61K38/1841 ,  A61K48/00

Abstract: Disclosed are TGF-60 mimetics that PEGylated TGF-&agr; polypeptides and PEGylated TGF-60 related polypetides or fragments thereof.

Abstract translation: 公开了PEG-TGF-α多肽和PEG化的TGF-60相关多肽或其片段的TGF-60模拟物。

公开(公告)号：US06663870B2

公开(公告)日：2003-12-16

申请号：US09823033

申请日：2001-03-29

Applicant: Charles E. Hart ,  Debra G. Gilbertson

Inventor： Charles E. Hart ,  Debra G. Gilbertson

IPC: A61K3818

CPC classification number: C07K14/52 ,  A01K2217/05 ,  A61K9/2027 ,  A61K38/1858 ,  A61K47/10 ,  A61K47/14 ,  A61K47/26 ,  A61K47/44 ,  A61L24/108 ,  A61L27/02 ,  A61L27/14 ,  A61L27/227 ,  A61L27/52 ,  A61L27/54 ,  A61L2300/252 ,  A61L2300/414 ,  A61L2300/43 ,  A61L2430/02 ,  A61L2430/10 ,  A61L2430/12 ,  C07K14/475 ,  C12N2799/022 ,  G01N2001/2223 ,  A61K2300/00

Abstract: Methods for promoting growth of bone, ligament, or cartilage in a mammal are disclosed. The methods comprise administering to said mammal a composition comprising a pharmacologically effective amount of a zvegf3 protein in combination with a pharmaceutically acceptable delivery vehicle. Also disclosed are methods for promoting proliferation or differentiation of osteoblasts, osteoclasts, chondrocytes, or bone marrow stem cells comprising culturing the cells in an effective amount of a zvegf3 protein.

Abstract translation: 公开了促进哺乳动物骨，韧带或软骨生长的方法。 所述方法包括向所述哺乳动物施用包含药理学有效量的zvegf3蛋白与药学上可接受的递送载体组合的组合物。 还公开了促进成骨细胞，破骨细胞，软骨细胞或骨髓干细胞的增殖或分化的方法，包括以有效量的zvegf3蛋白培养细胞。

公开(公告)号：US06656907B1

公开(公告)日：2003-12-02

申请号：US09518135

申请日：2000-03-03

Applicant: Andre G. Buret ,  D. Grant Gall ,  James A. Hardin ,  Merle E. Olson

Inventor： Andre G. Buret ,  D. Grant Gall ,  James A. Hardin ,  Merle E. Olson

IPC: A61K3818

CPC classification number: A61K31/277 ,  A23K20/147 ,  A23K20/168 ,  A23K50/00 ,  A23K50/30 ,  A61K38/1808

Abstract: New utilities for epidermal growth factor (EGF) are described. In particular, EGF is useful in preventing gastrointestinal colonization by pathogens.

Abstract translation: 描述了用于表皮生长因子（EGF）的新用途。 特别地，EGF可用于预防病原菌的胃肠定植。

公开(公告)号：US06559124B1

公开(公告)日：2003-05-06

申请号：US09076258

申请日：1998-05-12

Applicant: John S. O'Brien ,  Yasuo Kishimoto

Inventor： John S. O'Brien ,  Yasuo Kishimoto

IPC: A61K3818

CPC classification number: E03B7/074 ,  A61K9/0019 ,  A61K9/0048 ,  A61K9/0051 ,  A61K9/0085 ,  A61K9/127 ,  A61K9/19 ,  A61K38/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/47 ,  C07K14/475 ,  C07K14/52 ,  C07K14/705 ,  C07K14/71 ,  C07K14/715 ,  Y02A50/465

Abstract: Prosaposin, saposin C and various peptide fragments of saposin C stimulate neurite outgrowth in vitro. In addition, prosaposin and saposin C promote increased myelination ex vivo. Prosaposin is present in large neurons of the brain, including both upper and lower motor neurons.

Abstract translation: 皂角苷C和皂角苷C的各种肽片段在体外刺激神经突生长。 此外，赞子和皂角蛋白C促进离体髓鞘形成。 普罗沙星存在于脑的大神经元中，包括上部和下部运动神经元。

公开(公告)号：US06506729B1

公开(公告)日：2003-01-14

申请号：US08938622

申请日：1997-09-25

Applicant: David C. Rueger ,  Kuber T. Sampath ,  Charles M. Cohen ,  Hermann Oppermann ,  Roy H. L. Pang

Inventor： David C. Rueger ,  Kuber T. Sampath ,  Charles M. Cohen ,  Hermann Oppermann ,  Roy H. L. Pang

IPC: A61K3818

CPC classification number: C07K16/22 ,  A61F2310/00365 ,  A61K38/17 ,  A61K38/1703 ,  A61K38/1875 ,  A61L27/227 ,  A61L27/24 ,  C07K14/495 ,  C07K14/51 ,  G01N2500/10 ,  G01N2800/2835 ,  A61K2300/00

Abstract: Disclosed are therapeutic treatment methods, compositions and devices for maintaining neural pathways in a mammal, including enhancing survival of neurons at risk of dying, inducing cellular repair of damaged neurons and neural pathways, and stimulating neurons to maintain their differentiated phenotype. In one embodiment, the invention provides means for stimulating CAM expression in neurons. The invention also provides means for evaluating the status of nerve tissue, including means for detecting and monitoring neuropathies in a mammal. The methods, devices and compositions include a morphogen or morphogen-stimulating agent provided to the mammal in a therapeutically effective concentration.

Abstract translation: 公开了用于在哺乳动物中维持神经通路的治疗治疗方法，组合物和装置，包括增加有死亡风险的神经元的存活，诱导受损神经元和神经通路的细胞修复，以及刺激神经元维持其分化表型。 在一个实施方案中，本发明提供用于刺激神经元中的CAM表达的装置。 本发明还提供用于评估神经组织状态的手段，包括用于检测和监测哺乳动物神经病变的手段。 方法，装置和组合物包括以治疗有效浓度提供给哺乳动物的形态发生剂或形态发生剂。

公开(公告)号：US06506728B2

公开(公告)日：2003-01-14

申请号：US08450842

申请日：1995-05-26

Applicant: Arnon Rosenthal

Inventor： Arnon Rosenthal

IPC: A61K3818

CPC classification number: C07K14/475 ,  A61K38/00 ,  C07K16/22

Abstract: A novel polypeptide, designated neurotrophic factor-4 (NT-4), has been identified by PCR amplification of human genomic DNA. Provided herein is nucleic acid encoding NT-4 useful in diagnostics and in the recombinant preparation of NT-4. Also provided herein are nucleic acids encoding naturally occurring amino acid sequence variants of NT-4, designated NT-4&bgr;, NT-4&ggr;, and NT-4&Dgr;. The neurotrophic factors of the invention are useful in the treatment of nerve cells and in diagnostic assays.

Abstract translation: 已经通过人类基因组DNA的PCR扩增鉴定了一种称为神经营养因子-4（NT-4）的新型多肽。 本文提供了可用于诊断和NT-4重组制备中的NT-4的核酸。 本文还提供了编码NT-4，称为NT-4beta，NT-4gamma和NT-4DELTA的天然存在的氨基酸序列变体的核酸。 本发明的神经营养因子可用于神经细胞的治疗和诊断测定。

公开(公告)号：US06495520B2

公开(公告)日：2002-12-17

申请号：US09252656

申请日：1999-02-19

Applicant: Reinhard Ebner ,  Guo-Liang Yu ,  Steven M. Ruben ,  Jun Zhang ,  Stephen Ullrich ,  Yifan Zhai

Inventor： Reinhard Ebner ,  Guo-Liang Yu ,  Steven M. Ruben ,  Jun Zhang ,  Stephen Ullrich ,  Yifan Zhai

IPC: A61K3818

CPC classification number: C07K14/70575 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2799/026 ,  C12N2799/027

Abstract: The present invention relates to a novel member of the TNF-Ligand superfamily. More specifically, isolated nucleic acid molecules are provided encoding a human Apoptosis Inducing Molecule II (AIM II). AIM II polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of AIM II activity. Also provided are therapeutic methods for treating lymphadenopathy, aberrant bone development, autoimmune and other immune system diseases, graft versus host disease, rheumatoid arthritis, osteoarthritis and to inhibit neoplasia, such as tumor cell growth.

Abstract translation: 本发明涉及TNF-配体超家族的新成员。 更具体地，提供编码人细胞凋亡诱导分子II（AIM II）的分离的核酸分子。 还提供了AIM II多肽，载体，宿主细胞以及用于制备其的重组方法也是如此。 本发明还涉及用于鉴定AIM II活性的激动剂和拮抗剂的筛选方法。 还提供了治疗淋巴结病，异常骨发育，自身免疫和其他免疫系统疾病，移植物抗宿主病，类风湿性关节炎，骨关节炎和抑制肿瘤形成如肿瘤细胞生长的治疗方法。

公开(公告)号：US06451764B1

公开(公告)日：2002-09-17

申请号：US08706054

申请日：1996-08-30

Applicant: James Lee ,  William Wood

Inventor： James Lee ,  William Wood

IPC: A61K3818

CPC classification number: C07K16/22 ,  A61K38/00 ,  C07K14/52 ,  C07K14/71 ,  C07K2319/00 ,  C07K2319/30

Abstract: A human VEGF-related protein (VRP) has been identified and isolated that binds to, and stimulates the phosphorylation of, the receptor tyrosine kinase Flt4. The VRP is postulated to be a third member of the VEGF protein family. Also provided are antibodies that bind to VRP and neutralize a biological activity of VRP, compositions containing the VRP or antibody, methods of use, chimeric polypeptides, and a signal polypeptide for VRP.

Abstract translation: 已经鉴定并分离了与受体酪氨酸激酶Flt4结合并刺激受体酪氨酸激酶Flt4的磷酸化的人VEGF相关蛋白（VRP）。 假设VRP是VEGF蛋白家族的第三个成员。 还提供了结合VRP并中和VRP的生物活性的抗体，含有VRP或抗体的组合物，使用方法，嵌合多肽和用于VRP的信号多肽。

公开(公告)号：US06420339B1

公开(公告)日：2002-07-16

申请号：US09172644

申请日：1998-10-14

Applicant: Colin Gegg ,  Olaf Kinstler

Inventor： Colin Gegg ,  Olaf Kinstler

IPC: A61K3818

CPC classification number: A61K47/60 ,  Y10S514/909

Abstract: The present invention relates generally to the chemical modification of biologically active agents. More, specifically, the invention relates to a novel approach to engineer, through mutagenesis and site-directed chemical conjugation, specific, well-defined dualPEGylated-protein bioconjugates, consisting of two polyethylene glycol (PEG) macromolecules chemically conjugated to the protein at two specifically defined amino acid residues. The described dualPEGylated-protein bioconjugates show substantially improved bioefficacy and biocompatibility.

Abstract translation: 本发明一般涉及生物活性剂的化学修饰。 更具体地，本发明涉及通过诱变和定点化学共轭技术来设计特异性，明确定义的双重PEG化蛋白质生物共轭物的新方法，其由两个特异性地与蛋白质化学缀合的两个聚乙二醇（PEG）大分子组成 定义的氨基酸残基。 所描述的双重PEG化蛋白质生物共轭体显示出显着改善的生物功效和生物相容性。