公开(公告)号：US20100170400A1

公开(公告)日：2010-07-08

申请号：US12653506

申请日：2009-12-15

申请人： Juergen van den Boogard ,  Gerid Hellwig ,  Jens Ludwig ,  Oscar-Werner Reif ,  Stefan Weisshaar

发明人： Juergen van den Boogard ,  Gerid Hellwig ,  Jens Ludwig ,  Oscar-Werner Reif ,  Stefan Weisshaar

IPC分类号： B01D53/22

CPC分类号： C12M29/20

摘要： The invention relates to a waste gas system for bioreactors having a hydrophobic sterile filter, arranged at the end, and a heat exchanger which protects the sterile filter against blocking by liquid media. The gas outflow duct for discharging waste gas from a bioreactor vessel is divided in a cooling zone of the heat exchanger into a multiplicity of subducts and is extended in a heating zone of the heat exchanger to a calming zone. Due to the multiplicity of subducts, a considerably larger surface area for the removal of heat from the waste gas is available, with the result that in the case of laminar flow of the waste gas through the subducts a high effectiveness of the separation of liquids from the waste gas can be achieved. In the heating zone, the waste gas can heat up again due to the reduced flow rate until it reaches the hydrophobic sterile filter by way of taking up heat from the surroundings to a such a degree that the relative vapor contents are below 100% and separation of liquids from residual vapors at or in the hydrophobic sterile filter is precluded.

摘要翻译： 本发明涉及一种用于生物反应器的废气系统，其具有布置在其末端的疏水性无菌过滤器和保护无菌过滤器免受液体介质堵塞的热交换器。 用于从生物反应器容器排出废气的气体流出管在热交换器的冷却区中分成多个副产物，并在热交换器的加热区中延伸到一个镇定区。 由于多余的下降，可以获得相当大的用于从废气中除去热量的表面积，结果是在废气通过下沉层流的情况下，液体从液体中分离的高效率 可以实现废气。 在加热区中，废气可以由于流速降低而再次加热，直到其通过从周围环境吸收热量达到疏水性无菌过滤器至相对蒸汽含量低于100％的程度并且分离 的疏水性无菌过滤器中或其中残留蒸气的液体被排除。

公开(公告)号：US20090050213A1

公开(公告)日：2009-02-26

申请号：US11918107

申请日：2006-04-28

申请人： Christopher Biddell ,  Winfried Kuhlmann ,  Colin Kalman Lanyi ,  Oscar-Werner Reif

发明人： Christopher Biddell ,  Winfried Kuhlmann ,  Colin Kalman Lanyi ,  Oscar-Werner Reif

IPC分类号： A61M39/14 ,  F16L37/26 ,  A61M39/10

CPC分类号： A61M39/1011 ,  A61M39/14 ,  A61M39/18 ,  A61M2039/1027 ,  F16L37/26 ,  F16L2201/44 ,  Y10T137/0402

摘要： The invention relates to a connector (10) comprising: a fluid leadthrough (20); a contact device (14); an essentially planar contact surface (16), and; a fluid leadthrough opening (18), which is designed for leading fluid though, the fluid leadthrough opening (18) passing through the contact surface (16); a sterile covering (64) designed for sterilely covering, at least in part, the contact surface (16) and/or an end of the fluid leadthrough opening (20) facing the contact surface, and; a coupling device (22), which is designed for engaging with a complementary coupling device (34) of a complementary connector (12) along a coupling direction, the coupling device (40) being essentially parallel to the contact surface (16). The invention also relates to relates to a connector system, to a use of a connector (10) and to a complementary connector (12) for connecting a fluid supply of the connector (10) to a fluid discharge in a sterile manner.

摘要翻译： 本发明涉及一种连接器（10），包括：流体引线（20）; 接触装置（14）; 基本上平面的接触表面（16），和 流体通过开口（18），其被设计用于引导流体通过所述流体穿通开口（18），所述流体穿通开口穿过所述接触表面（16）; 至少部分地无菌地覆盖面向接触表面的接触表面（16）和/或流体引导开口（20）的端部的无菌覆盖物（64），以及; 联接装置（22），其被设计用于沿着联接方向与互补连接器（12）的互补联接装置（34）接合，所述联接装置（40）基本上平行于所述接触表面（16）。 本发明还涉及连接器系统，使用连接器（10）和用于以无菌方式将连接器（10）的流体供应件连接到流体排放口的互补连接器（12）。

公开(公告)号：US20080213753A1

公开(公告)日：2008-09-04

申请号：US12047470

申请日：2008-03-13

申请人： Hartmut HENNING ,  Oscar-Werner Reif ,  Klaus Tarrach ,  Robert Zeidler

发明人： Hartmut HENNING ,  Oscar-Werner Reif ,  Klaus Tarrach ,  Robert Zeidler

IPC分类号： C12Q1/70

CPC分类号： C12N7/00 ,  C12N2750/14051

摘要： Disclosed is a method for verification of the removal of viruses to validate filters, filtering processes, physical and chemical inactivation processes, or adsorptive removal processes in predefined process conditions that are simulated on a small scale. According to said method, viruses are cultured in suitable cell lines in a first step, a virus suspension is obtained after solubilizing cells in a second step, the virus suspension obtained is added in a third step to a protein solution that is to be analyzed, and the virus-containing protein solution is filtered through the filter that is to be validated, and the removal of viruses is then analyzed in a fourth step. The virus suspension is first processed via a membrane adsorber following step two, the viruses being bonded to the membrane adsorber and contaminants being removed with the aid of a detergent buffer solution, while the bonded, purified viruses are eluted from the membrane adsorber area and are added to the protein solution that is to be analyzed as a concentrated virus suspension in step three.

摘要翻译： 公开了一种验证病毒去除以验证过滤器，过滤过程，物理和化学灭活过程或在小规模模拟的预定义工艺条件下的吸附去除过程的方法。 根据所述方法，在第一步骤中，将病毒在合适的细胞系中培养，在第二步溶解细胞后获得病毒悬浮液，将获得的病毒悬浮液添加到待分析的蛋白质溶液中， 并通过要验证的过滤器过滤病毒蛋白质溶液，然后在第四步骤中分析病毒的去除。 首先在步骤2之后通过膜吸附器处理病毒悬浮液，病毒结合到膜吸附器上，借助于洗涤剂缓冲溶液除去污染物，而结合的纯化病毒从膜吸附区域洗脱出来，并且是 添加到在步骤3中待浓缩的病毒悬浮液分析的蛋白质溶液中。

公开(公告)号：US20070227270A1

公开(公告)日：2007-10-04

申请号：US11713978

申请日：2007-03-05

申请人： Heyo Mennenga ,  Oscar-Werner Reif ,  Dieter Schmidt ,  Reinhard Vogt

发明人： Heyo Mennenga ,  Oscar-Werner Reif ,  Dieter Schmidt ,  Reinhard Vogt

IPC分类号： G01N1/14

CPC分类号： G01N35/1079 ,  A61J1/12 ,  A61J1/20 ,  C12M33/00 ,  C12M37/04 ,  G01N2001/1056

摘要： The invention discloses an apparatus for removing samples or introducing fluids from/into system(s) having flexible walls. A sampler/fluid dispenser (3) is fixed on the outside with a connecting area (4) of its base (5) on a sample removal region/fluid dispensing region (6) of the flexible wall (2) and, in its interior, has a severing element (8). At least the severing element (8) can be pushed forward in the direction of the sample removal region/fluid dispensing region (6) in order to remove the samples and in order to introduce fluids, in such a way that the wall (2) can be perforated within the sample removal region/fluid dispensing region (6). The system (1) comprises containers, bags or tubes for filling, mixing, transporting, storage of media, such as biological liquids, sera, buffers, ultra-pure water and can be used as biological reactors or cell culture vessels in the pharmaceutical and biotechnical industry.

摘要翻译： 本发明公开了一种用于从具有柔性壁的系统中去除样品或将流体从/进入系统的装置。 取样器/流体分配器（3）在其外部固定有其基部（5）的连接区域（4）在柔性壁（2）的样品移除区域/流体分配区域（6）上，并且在其内部 ，具有切断要素（8）。 至少切断元件（8）可以沿着样品移除区域/流体分配区域（6）的方向向前推动，以便去除样品并且以这样的方式引入流体：壁（2） 可以在样品去除区域/流体分配区域（6）内穿孔。 系统（1）包括用于填充，混合，运输，储存诸如生物液体，血清，缓冲液，超纯水的介质的容器，袋或管，并可用作药物和/或制剂中的生物反应器或细胞培养容器 生物工业。

公开(公告)号：US20070178576A1

公开(公告)日：2007-08-02

申请号：US10587064

申请日：2005-01-27

申请人： Wolfgang Demmer ,  Matthias Grabosch ,  Oscar-Werner Reif ,  Wolfgan Nagel ,  Mariel Donzeau ,  Peter Reichelt

发明人： Wolfgang Demmer ,  Matthias Grabosch ,  Oscar-Werner Reif ,  Wolfgan Nagel ,  Mariel Donzeau ,  Peter Reichelt

IPC分类号： C12N7/02 ,  C07K14/47 ,  C12N5/06

CPC分类号： C12N7/00 ,  A61K2039/5256 ,  C07K1/22 ,  C07K14/005 ,  C12N2795/14122 ,  C12N2795/14151

摘要： The invention relates to a method for purifying and/or isolating high-molecular compounds, such as high-molecular proteins or protein-type compounds, contained in a solution or a suspension, by means of affinity chromatography using membranes containing metal ions.

摘要翻译： 本发明涉及通过使用含有金属离子的膜的亲和层析来纯化和/或分离包含在溶液或悬浮液中的高分子化合物，例如高分子蛋白质或蛋白质类型化合物的方法。

公开(公告)号：US06959812B2

公开(公告)日：2005-11-01

申请号：US10214896

申请日：2002-08-07

申请人： Oscar-Werner Reif ,  Andreas Graus

发明人： Oscar-Werner Reif ,  Andreas Graus

IPC分类号： A61M5/165 ,  B65D77/20 ,  B65D75/64

CPC分类号： A61M5/002 ,  A61M5/165 ,  A61M5/3145 ,  A61M2209/06 ,  B65D2577/2091

摘要： There is disclosed a filter package comprising a housing and a cover that is adapted to enclose a sterile disposable filter wherein the package cover is penetrable at a point axially aligned with the filter and has at least one pre-formed break line along which the package cover, when subjected to appropriate force, ruptures so as to permit withdrawal of the filter by a syringe coupled thereto.

摘要翻译： 公开了一种过滤器包装，其包括壳体和盖，其适于包围无菌一次性过滤器，其中包装盖可在与过滤器轴向对准的点处穿透，并且具有至少一个预先形成的断裂线，包装盖 当受到适当的力时，破裂以允许通过与其连接的注射器抽出过滤器。

公开(公告)号：US06186341B1

公开(公告)日：2001-02-13

申请号：US09083562

申请日：1998-05-22

申请人： Peter Konstantin ,  Oscar-Werner Reif ,  Jürgen Rupp ,  Peter Soelkner

发明人： Peter Konstantin ,  Oscar-Werner Reif ,  Jürgen Rupp ,  Peter Soelkner

IPC分类号： B01D2900

CPC分类号： B01D63/061 ,  B01D63/067 ,  B01D65/003

摘要： The hydrophilicity of porous separation membranes is maintained by bonding the membranes to thermoplastic fiber substrates at least on their peripheries before the membrane peripheries are sealed in a thermoplastic resin. The membranes are capable of being integrity tested.

摘要翻译： 多孔分离膜的亲水性通过在膜周围被密封在热塑性树脂中之前至少在它们的周围粘合到热塑性纤维基材来保持。 膜能够进行完整性测试。