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    Hybridization-based fluorescence assay
    71.
    发明申请
    Hybridization-based fluorescence assay 审中-公开
    基于杂交的荧光测定

    公开(公告)号：US20050089915A1

    公开(公告)日：2005-04-28

    申请号：US10974129

    申请日：2004-10-27

    申请人： Ekambar Kandimalla ,  Sudhir Agrawal

    发明人： Ekambar Kandimalla ,  Sudhir Agrawal

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2563/173 ,  C12Q2537/107

    摘要： The invention provides a method for detecting the presence or absence of an oligonucleotide in a sample. In the method according to the invention, a sample is obtained that may contain a target oligonucleotide. An oligonucleotide that is complementary to the target oligonucleotide and a fluorophore are added to the sample to form a mixture. The mixture is then incubated under hybridization conditions to allow binding of the complementary oligonucleotide to the target oligonucleotide to form a duplex. The fluorophore binds to the duplex; and fluorescence is then detected by standard procedures. The detection of fluorescence is indicative of the presence of the target oligonucleotide in the sample.

    摘要翻译： 本发明提供一种检测样品中寡核苷酸存在或不存在的方法。 在根据本发明的方法中，获得可以含有靶寡核苷酸的样品。 将与靶寡核苷酸和荧光团互补的寡核苷酸加入到样品中以形成混合物。 然后将混合物在杂交条件下孵育，以使互补寡核苷酸与靶寡核苷酸结合形成双链体。 荧光团与双链体结合; 然后通过标准程序检测荧光。 荧光的检测表明样品中靶寡核苷酸的存在。

    Triplex-forming antisense oligonucleotides having abasic linkers targeting nucleic acids comprising mixed sequences of purines and pyrimidines
    80.
    发明授权
    Triplex-forming antisense oligonucleotides having abasic linkers targeting nucleic acids comprising mixed sequences of purines and pyrimidines 失效
    具有靶向包含嘌呤和嘧啶的混合序列的核酸的碱基接头的三重反应形成反义寡核苷酸

    公开(公告)号：US5693773A

    公开(公告)日：1997-12-02

    申请号：US473096

    申请日：1995-06-07

    申请人： Ekambar Kandimalla ,  Sudhir Agrawal

    发明人： Ekambar Kandimalla ,  Sudhir Agrawal

    IPC分类号： A61K38/00 ,  C07H21/00 ,  C12N15/113 ,  C07H19/00 ,  C07H21/04 ,  C12Q1/68 ,  A61K48/00

    CPC分类号： C12N15/113 ,  C07H21/00 ,  C12N15/1132 ,  A61K38/00 ,  C12N2310/152 ,  C12N2310/3183 ,  C12N2310/332

    摘要： The present invention provides a novel class of antisense oligonucleotides capable of hybridizing to and inhibiting expression of nucleic acids having mixed purine/pyrimidine sequences by triplex formation. The foldback triplex-forming oligonucleotides (FTFOs) of the invention are comprised of three regions, a duplex-forming region, which is sufficiently complementary to a region of the target nucleic acid to hybridizes to it under the conditions of interest, a triplex-forming region, which is an inverted repeat of the duplex-forming region and folds back upon the duplex formed between the duplex-forming region and the target nucleic acid to form a triplex, and a linker region, which connects the duplex-forming region and the triplex-forming region and allows formation of the triplex. A novel aspect of the FTFOs of the present invention is that from one to five abasic linkers substitute for nucleotides in the triplex-forming region and are positioned to match up with pyrimidine residues of the target when a triplex is formed. This allows the FTFOs of the present invention to target nucleic acid sequences having mixed purine/pyrimidine sequences. FTFOs according to the invention are useful for both in vitro and in vivo modulation of gene expression.

    摘要翻译： 本发明提供了能够通过三联体形成杂交并抑制具有混合的嘌呤/嘧啶序列的核酸的表达的新一类反义寡核苷酸。 本发明的折叠三重复合形成寡核苷酸（FTFO）由三个区域组成，双相形成区域与靶核酸的区域充分互补，以在感兴趣的条件下与其杂交，三重生成 区域，其是双链体形成区域的反向重复并且折叠在双链体形成区域和靶核酸之间形成的双链体上以形成三链体，以及连接区域，其连接双相形成区域和 三重形成区域并允许形成三链体。 本发明的FTFO的一个新颖方面是从形成三链体形成区域中的1至5个碱基接头代替核苷酸，并且当形成三链体时被定位成与靶标的嘧啶残基相匹配。 这允许本发明的FTFO靶向具有混合嘌呤/嘧啶序列的核酸序列。 根据本发明的FTFO可用于基因表达的体外和体内调节。