Mutant human hepatitis B viral strain and uses thereof
    72.
    发明授权
    Mutant human hepatitis B viral strain and uses thereof 失效
    突变型人乙型肝炎病毒株及其用途

    公开(公告)号:US07105165B2

    公开(公告)日:2006-09-12

    申请号:US10761006

    申请日:2004-01-20

    IPC分类号: A39 C12N7/01 C12N7/02

    摘要: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide. This invention also provides various methods of using the disclosed isolated nucleic acid and peptides.

    摘要翻译: 本发明提供了一种分离的乙型肝炎病毒株,其命名为人乙型肝炎病毒表面抗原-'S'-133 Oon菌株(甲硫氨酸至苏氨酸),构成病毒基因组以保藏号P97121501,P97121502和P97121503保藏在欧洲集合 细胞培养在15日/ 12月12日。 本发明还提供了编码多肽的分离核酸,其是乙型肝炎病毒株的突变型主要表面抗原,该多肽具有不同于野生型主要表面抗原的氨基酸序列的氨基酸序列 因为该多肽的第133位氨基酸是苏氨酸,而不是甲硫氨酸。 本发明还提供了编码肽的分离的核酸,其中肽由包含SEQ ID NO:5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供使用所公开的分离的核酸和肽的各种方法。

    Vaccine-induced hepatitis B viral strain and uses thereof
    73.
    发明授权
    Vaccine-induced hepatitis B viral strain and uses thereof 失效
    疫苗诱导的乙型肝炎病毒株及其用途

    公开(公告)号:US07038035B1

    公开(公告)日:2006-05-02

    申请号:US09719533

    申请日:1998-06-19

    摘要: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-'Ś-145 Singapore Strain (Glycine to Arginine) which constituent viral genome is deposited under Accession Nos. P97121504, P97121505 and P97121506 with the European Collection of Cell Culture on 15th Dec. 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 145 of such polypeptide is an arginine rather than a glycine, and the purified peptide. This invention further provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. ID. No. 1, and the purified peptide. This invention also provides various methods using the disclosed isolated nucleic acids and polypeptides. This invention also provides various uses of the viral strain and its proteins.

    摘要翻译: 本发明提供了一种分离的乙型肝炎病毒株,命名为人乙型肝炎病毒表面抗原-145新加坡菌株(Glycine to Arginine),其构成病毒基因组以保藏号P97121504,P97121505和P97121506与欧洲细胞集合 本发明还提供了编码多肽的分离核酸,其是乙型肝炎病毒株的突变型主要表面抗原,该多肽具有不同于主要的氨基酸序列的氨基酸序列 野生型乙型肝炎病毒的表面抗原是这种多肽的位置编号145的氨基酸是精氨酸而不是甘氨酸,和纯化的肽。 本发明还提供了编码肽的分离的核酸,其中所述肽由包含SEQ ID NO:5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供了使用公开的分离的核酸和多肽的各种方法。 本发明还提供了病毒株及其蛋白质的各种用途。

    System and method for assessing TV-related information over the internet
    74.
    发明授权
    System and method for assessing TV-related information over the internet 有权
    通过互联网评估电视相关信息的系统和方法

    公开(公告)号:US06901366B1

    公开(公告)日:2005-05-31

    申请号:US09383763

    申请日:1999-08-26

    CPC分类号: G06F17/30663 G10L2015/228

    摘要: The system retrieves information from the internet using multiple search engines that are simultaneously launched by the search engine commander. The commander is responsive to a speech-enabled system including a speech recognizer and natural language parser. The user speaks to the system in natural language requests, and the parser extracts the semantic content from the user's speech, based on a set of goal oriented grammars. The preferred system includes a fixed grammar and an updatable or downloaded grammar, allowing the system to be used without extensive training and yet capable of being customized for a particular user's purposes. Results obtained from the search engines are filtered based on information extracted from an electronic program guide and from prestored user profile data. The results may be displayed on screen or through synthesized speech.

    摘要翻译: 系统使用搜索引擎指挥官同时启动的多个搜索引擎从互联网检索信息。 指挥官对包括语音识别器和自然语言解析器的支持语音的系统做出响应。 用户以自然语言请求对系统说话,并且解析器基于一组面向目标的语法从用户的语音中提取语义内容。 优选的系统包括固定语法和可更新或下载的语法,允许系统在没有广泛训练的情况下使用,并且能够根据特定用户的目的进行定制。 从搜索引擎获得的结果根据从电子节目指南提取的信息和预先存储的用户简档数据进行过滤。 结果可能会显示在屏幕上或通过合成语音显示。

    Mutant human hepatitis B viral strain and uses thereof
    75.
    发明授权
    Mutant human hepatitis B viral strain and uses thereof 失效
    突变型人乙型肝炎病毒株及其用途

    公开(公告)号:US06787142B2

    公开(公告)日:2004-09-07

    申请号:US10209264

    申请日:2002-07-31

    IPC分类号: A61K3929

    摘要: This invention provides an isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) which constituent viral genome is deposited under Accession Nos. P97121501, P97121502 and P97121503 with the European Collection of Cell Culture on 15th December 1997. This invention also provides an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than a methionine. This invention also provides an isolated nucleic acid which encodes a peptide, wherein the peptide is encoded by a nucleic acid molecule comprising nucleotides 527 through 595 of SEQ. I.D. No. 1 and the purified peptide. This invention also provides various methods of using the disclosed isolated nucleic acid and peptides.

    摘要翻译: 本发明提供了一种分离的乙型肝炎病毒株,其命名为人乙型肝炎病毒表面抗原-'S'-133 Oon菌株(甲硫氨酸至苏氨酸),构成病毒基因组以保藏号P97121501,P97121502和P97121503保藏在欧洲集合 细胞培养。本发明还提供编码多肽的分离核酸,其是乙型肝炎病毒株的突变型主要表面抗原,该多肽具有不同于氨基酸序列的氨基酸序列 的野生型乙型肝炎病毒的主要表面抗原,因为该多肽的第133位的氨基酸是苏氨酸而不是甲硫氨酸。 本发明还提供了编码肽的分离的核酸,其中肽由包含SEQ ID NO:5的核苷酸527至595的核酸分子编码。 ID。 1和纯化肽。 本发明还提供使用所公开的分离的核酸和肽的各种方法。

    Mutant human hepatitis B viral strain and uses thereof
    76.
    发明授权
    Mutant human hepatitis B viral strain and uses thereof 失效
    突变型人乙型肝炎病毒株及其用途

    公开(公告)号:US06558675B1

    公开(公告)日:2003-05-06

    申请号:US09719528

    申请日:2001-04-30

    IPC分类号: A61K3912

    摘要: An isolated strain of Hepatitis B virus designated Human Hepatitis B Virus Surface Antigen-‘S’-133 Oon Strain (Methionine to Threonine) of Cell. Also, an isolated nucleic acid encoding a polypeptide which is a mutant major surface antigen of a strain of hepatitis B virus, such polypeptide having an amino acid sequence which differs from the amino acid sequence of a major surface antigen of a wild type hepatitis B virus in that the amino acid at position number 133 of such polypeptide is a threonine rather than methionine.

    摘要翻译: 一种分离的乙型肝炎病毒株,命名为人类乙型肝炎病毒表面抗原 - 细胞的“S'-133 Oon菌株(甲硫氨酸至苏氨酸)”。此外,编码多肽的分离的核酸是多肽的突变主要表面抗原 乙型肝炎病毒,这种多肽具有与野生型乙型肝炎病毒的主要表面抗原的氨基酸序列不同的氨基酸序列,因为该多肽的第133位的氨基酸是苏氨酸而不是甲硫氨酸。

    Speech detection for noisy conditions
    77.
    发明授权
    Speech detection for noisy conditions 失效
    嘈杂条件的语音检测系统

    公开(公告)号:US06480823B1

    公开(公告)日:2002-11-12

    申请号:US09047276

    申请日:1998-03-24

    IPC分类号: G10L2102

    CPC分类号: G10L25/87

    摘要: The input signal is transformed into the frequency domain and then subdivided into bands corresponding to different frequency ranges. Adaptive thresholds are applied to the data from each frequency band separately. Thus the short-term band-limited energies are tested for the presence or absence of a speech signal. The adaptive threshold values are independently updated for each of the signal paths, using a histogram data structure to accumulate long-term data representing the mean and variance of energy within the respective frequency band. Endpoint detection is performed by a state machine that transitions from the speech absent state to the speech present state, and vice versa, depending on the results of the threshold comparisons. A partial speech detection system handles cases in which the input signal is truncated.

    摘要翻译: 将输入信号变换为频域,然后再分为对应于不同频率范围的频带。 自适应阈值分别应用于每个频带的数据。 因此,测试短期带限能量是否存在语音信号。 使用直方图数据结构来累积表示各个频带内的能量的平均值和方差的长期数据,针对每个信号路径独立地更新自适应阈值。 端点检测由状态机执行,状态机从语音不存在状态转换到语音存在状态,反之亦然,这取决于阈值比较的结果。 部分语音检测系统处理输入信号被截断的情况。