公开(公告)号：US20080187922A1

公开(公告)日：2008-08-07

申请号：US11871932

申请日：2007-10-12

Applicant: Honghai Wang ,  Lu Zhang ,  Qingzhong Wang ,  Jun Yue ,  Min Zhang ,  Ying Xu ,  Bingdong Zhu ,  Jiuling Wang ,  Yaqing Qie

Inventor： Honghai Wang ,  Lu Zhang ,  Qingzhong Wang ,  Jun Yue ,  Min Zhang ,  Ying Xu ,  Bingdong Zhu ,  Jiuling Wang ,  Yaqing Qie

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/18 ,  C12Q2600/156 ,  G01N33/5695 ,  G01N2333/35 ,  G01N2500/00

Abstract: The present invention belongs to the field of molecular biology. Specially, the invention relates to the screenings of a new drug target, a vaccine antigen and a test target of anti-tuberculosis treatment. Specially, based on the differential proteome technology and by the techniques of gene sequencing, in vitro protein expression, immunological detection and gene recombination, the invention primarily certifies that the up-regulated expression of Protein Rv2629 and the mutation of Gene 191A/C of Mycobacterium tuberculosis are related to the Rifampicin (RFP) drug-resistance, which provides useful information for the drug-resistance detection of Mycobacterium tuberculosis, the novel drug design and the vaccine development. Firstly, the drug-resistant strains are cultured and the proteins from drug resistance strains and sensitive strains are isolated respectively. Secondly, the proteins from the drug resistance strains and the sensitive strains are compared to determine the differential protein sites, such that the drug-resistant proteins are identified by means of Mass Spectrometry. Next, the up-regulated expression of Protein Rv2629 and the mutation of gene are correlated to the drug-resistance of Mycobacterium tuberculosis by means of gene sequencing technique. Finally, after the gene cloning, the in vitro expression and purification of the protein, the wild-type and mutant-type recombinant protein of Rv2629 are obtained. The polyclonal antibody is prepared, and the protein subcellular localization is carried out. The Minimum Inhibitory Concentrations (MICs) of the strains containing different plasmids obtained by the transformation of recombinant shuttle plasmids are also measured. The gene encoding the protein Rv2629 in the genome of Mycobacterium tuberculosis is isolated and is up-regulation expressed in a Rifampicin (RFP)-resistant strains. And the A/C gene mutation at nucleotide 191 which is related to drug-resistance is detected. The relationship between the mutation and the Rifampicin (RFP)-resistance is primarily certified by molecular biology technique and the in vitro experiments. This invention provides methods used to accurately isolate and identify the drug-resistance protein of Mycobacterium tuberculosis. The A/C mutation of Rv2629 at nucleotide 191 related to the drug-resistance of Mycobacterium tuberculosis is isolated, which shows new approach for the further study of Mycobacterium tuberculosis drug-resistant mechanism, the rapid detection of clinical drug-resistance strains, as well as the developments of new drug targets and vaccines.

公开(公告)号：US20080182764A1

公开(公告)日：2008-07-31

申请号：US11669060

申请日：2007-01-30

Applicant: Ying Xu ,  B. R. Reddy ,  Rickey Morgan ,  Lance Brothers

Inventor： Ying Xu ,  B. R. Reddy ,  Rickey Morgan ,  Lance Brothers

IPC: C09K3/00

CPC classification number: C09K8/467 ,  C04B28/06 ,  C04B2103/10 ,  Y02W30/92 ,  C04B18/08 ,  C04B22/085 ,  C04B22/124 ,  C04B22/16 ,  C04B24/06 ,  C04B2103/0006 ,  C04B2103/001

Abstract: A method of servicing a wellbore in contact with a subterranean formation, comprising placing into a wellbore a composition comprising a calcium aluminate cement and a polyvalent cation-containing compound, and allowing the composition to set. A method of increasing the thickening time of a calcium aluminate cement comprising contacting the calcium aluminate cement with a polyvalent cation-containing compound. A method of adjusting the thickening time of a calcium aluminate cement comprising contacting the calcium aluminate cement with a polyvalent cation-containing compound and at least one other set modifier.

Abstract translation: 一种维护与地层接触的井筒​​的方法，包括将包含铝酸钙水泥和多价阳离子的化合物的组合物放入井筒中，并使组合物凝固。 一种增加铝酸钙水泥的增稠时间的方法，包括使铝酸钙水泥与多价阳离子化合物接触。 一种调节铝酸钙胶泥的增稠时间的方法，其包括使铝酸钙水泥与含有多价阳离子的化合物和至少一种其它组合改性剂接触。

公开(公告)号：US06715336B1

公开(公告)日：2004-04-06

申请号：US10374120

申请日：2003-02-24

Applicant: Ying Xu

Inventor： Ying Xu

IPC: G01N356

CPC classification number: F16C19/52 ,  G01M7/04 ,  G01N3/56 ,  G01N2203/0051

Abstract: Disclosed are a device and a corresponding method to test the fretting of a ball bearing under precisely controlled conditions. The device employs a first piezoelectric actuator to deliver a pre-determined force to the surface of a ball bearing or other object to be tested. The force is applied in a first axis of motion. A second piezoelectric actuator, coupled to a flexure hinge platform, translates the workpiece in a second axis of motion that is different (and preferably substantially orthogonal) to the first axis. The combination of the relative motion between the workpiece and the force exerted upon it produces predetermined dynamic patterns of the stress and strain on the surfaces of the workpiece being tested. Closed-loop control architecture, piezoelectric actuators, and force/displacement sensors included in the device provide highly accurate and rapid tests.

Abstract translation: 公开了一种在精确控制的条件下测试滚珠轴承的磨损的装置和相应的方法。 该装置采用第一压电致动器将预定的力传递到球轴承或其它被测物体的表面。 该力被施加在第一运动轴上。 耦合到挠性铰链平台的第二压电致动器使工件在与第一轴线不同（且优选地基本正交）的第二运动轴线上平移。 工件与施加在其上的力之间的相对运动的组合在被测工件的表面上产生应力和应变的预定动态图案。 设备中包含的闭环控制架构，压电执行器和力/位移传感器可提供高精度和快速的测试。