公开(公告)号：US20210262042A1

公开(公告)日：2021-08-26

申请号：US17314099

申请日：2021-05-07

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Maria Giakoumopoulos ,  David A. Ahlquist ,  William R. Taylor ,  Douglas Mahoney

IPC: C12Q1/6886 ,  C12Q1/6806

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US11091812B2

公开(公告)日：2021-08-17

申请号：US16569533

申请日：2019-09-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12P19/34 ,  C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6881

Abstract: A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided.

公开(公告)号：US11078539B2

公开(公告)日：2021-08-03

申请号：US15301227

申请日：2015-03-26

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  Douglas W. Mahoney ,  Graham P. Lidgard ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.

公开(公告)号：US20210189462A1

公开(公告)日：2021-06-24

申请号：US17174127

申请日：2021-02-11

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US11028447B2

公开(公告)日：2021-06-08

申请号：US16460647

申请日：2019-07-02

Applicant: Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim Allawi ,  Graham P. Lidgard ,  Maria Giakoumopoulos ,  David A. Ahlquist ,  William R. Taylor ,  Douglas Mahoney

IPC: C12Q1/6886 ,  C12Q1/6806

Abstract: Provided herein is technology for lung neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of lung cancer.

公开(公告)号：US20210130911A1

公开(公告)日：2021-05-06

申请号：US17144806

申请日：2021-01-08

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： David A. Ahlquist ,  William R. Taylor ,  John B. Kisiel ,  Tracy C. Yab ,  Douglas W. Mahoney ,  Brian A. Dukek ,  Matthew T. Gettman ,  Hatim T. Allawi

IPC: C12Q1/6886

Abstract: Provided herein is technology for prostate cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of prostate cancer.

公开(公告)号：US20210130907A1

公开(公告)日：2021-05-06

申请号：US17085542

申请日：2020-10-30

Applicant: Mayo Foundation for Medical Education and Research ,  Exact Sciences Development Company, LLC

Inventor： William R. Taylor ,  John B. Kisiel ,  Douglas W. Mahoney ,  David A. Ahlquist ,  Hatim T. Allawi ,  Michael W. Kaiser

IPC: C12Q1/6886 ,  C12N15/117 ,  C12Q1/6869 ,  G01N33/50

Abstract: Provided herein is technology for ovarian cancer screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of ovarian cancer and sub-types of ovarian cancer (e.g., clear cell ovarian cancer, endometrioid ovarian cancer, mucinous ovarian cancer, serous ovarian cancer).

公开(公告)号：US10954556B2

公开(公告)日：2021-03-23

申请号：US16134254

申请日：2018-09-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US10889867B2

公开(公告)日：2021-01-12

申请号：US16168326

申请日：2018-10-23

Applicant: Exact Sciences Development Company, LLC

Inventor： Ilse A. Tyler ,  Keith Yaeger ,  Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12N15/11 ,  C12Q1/6876

Abstract: The present invention provides synthetic DNA strands that find use as controls or in nucleic acid testing methods. In particular, provided herein are synthetic DNA strands of known composition for use as control molecules in stool DNA testing, e.g., of mutations and/or methylation of DNA isolated from stool samples.

公开(公告)号：US20200291459A1

公开(公告)日：2020-09-17

申请号：US16888397

申请日：2020-05-29

Applicant: Exact Sciences Development Company, LLC

Inventor： Michael J. Domanico ,  Hatim Allawi ,  Graham P. Lidgard ,  Brian Aizenstein ,  Oliver Hunt ,  Tobias Charles Zutz

IPC: C12Q1/6806 ,  C12Q1/6851

Abstract: Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.