公开(公告)号：US20180208631A1

公开(公告)日：2018-07-26

申请号：US15868676

申请日：2018-01-11

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  David J. Bowen ,  Catherine A. Chay ,  David J. Chi ,  William P. Clinton ,  Crystal L. Dart ,  Leigh English ,  Stanislaw Flasinski ,  Victor M. Guzov ,  Kevin A. Jarrell ,  Uma R. Kesanapalli ,  Thomas M. MaIvar ,  Robert M. McCarroll ,  Jason S. Milligan ,  Jay P. Morgenstern ,  Deborah G. Rucker ,  Sara A. Salvador ,  Temple F. Smith ,  Carlos E. Soto ,  Collin M. Stultz ,  Brian M. Turczyk ,  Ty T. Vaughn ,  Moritz W.F.F. Von Rechenberg

IPC: C07K14/325 ,  C12N15/82 ,  A01N63/02 ,  A01N37/46

Abstract: Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

公开(公告)号：US09957524B2

公开(公告)日：2018-05-01

申请号：US14671542

申请日：2015-03-27

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Uma R. Kesanapalli ,  Jason S. Milligan ,  Rachael N. Slightom ,  Daqi Tu ,  Andrew M. Wollacott ,  Yong Yin

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: The invention generally relates to the field of insect inhibitory toxin proteins. A novel class of proteins exhibiting insect inhibitory activity against agriculturally relevant pests of crop plants and seeds are disclosed. Insecticidal activity is particularly effective against the Coleopteran order of insect pests. Plants, plant parts, and seed are provided containing a polynucleotide construct encoding one or more of the toxin proteins disclosed herein. The proteins are referred to herein variously as the TIC2463-related toxin protein class or family, the TIC2463-related toxin proteins, the TIC2463-related protein genus, toxin proteins related to the TIC2463 toxin protein, proteins related to TIC2463, TIC2463-related toxin polypeptides, TIC2463-related pesticidal proteins, and the like.

公开(公告)号：US20170137842A1

公开(公告)日：2017-05-18

申请号：US15230112

申请日：2016-08-05

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Timothy D. Panosian ,  Yong Yin

IPC: C12N15/82 ,  A01N63/02 ,  C07K14/325 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/195 ,  C07K14/325 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  G01N33/68 ,  G01N2333/325

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC2421 TIC3008, and TIC7055. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC2421, TIC3008, and TIC7055 pesticidal proteins are also provided.

公开(公告)号：US20160319302A1

公开(公告)日：2016-11-03

申请号：US14945140

申请日：2015-11-18

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  A01N63/02 ,  C07K14/195

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

Abstract translation: 公开了对鞘翅目和鳞翅目害虫物质具有毒性活性的杀虫蛋白，包括但不限于TIC3668，TIC3669，TIC3670，TIC4076，TIC4078，TIC4260，TIC4346，TIC4826，TIC4861，TIC4862，TIC4863和TIC-3668- 型蛋白。 提供DNA分子和构建体，其含有编码一种或多种所公开的TIC3668型蛋白质的多核苷酸序列。 提供了转基因植物，植物细胞，种子和对鳞翅目和鞘翅目昆虫侵染的植物部分，其含有编码本发明的杀虫蛋白的多核苷酸序列。 还提供了用于检测生物样品中本发明的多核苷酸或蛋白质的存在的方法，以及使用任何TIC3668型杀虫蛋白质来控制鞘翅目和鳞翅目昆虫害虫的方法。

公开(公告)号：US20150274786A1

公开(公告)日：2015-10-01

申请号：US14671542

申请日：2015-03-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Uma R. Kesanapalli ,  Jason S. Milligan ,  Rachael N. Slightom ,  Daqi Tu ,  Andrew M. Wollacott ,  Yong Yin

IPC: C07K14/325 ,  C12N15/82

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: The invention generally relates to the field of insect inhibitory toxin proteins. A novel class of proteins exhibiting insect inhibitory activity against agriculturally relevant pests of crop plants and seeds are disclosed. Insecticidal activity is particularly effective against the Coleopteran order of insect pests. Plants, plant parts, and seed are provided containing a polynucleotide construct encoding one or more of the toxin proteins disclosed herein. The proteins are referred to herein variously as the TIC2463-related toxin protein class or family, the TIC2463-related toxin proteins, the TIC2463-related protein genus, toxin proteins related to the TIC2463 toxin protein, proteins related to TIC2463, TIC2463-related toxin polypeptides, TIC2463-related pesticidal proteins, and the like.

Abstract translation: 本发明一般涉及昆虫抑制毒素蛋白质的领域。 公开了一类新的对农作物和种子的农业相关害虫的抑制活性的蛋白质。 杀虫活性对昆虫有害生物的鞘翅目顺序特别有效。 提供植物，植物部分和种子，其含有编码本文公开的一种或多种毒素蛋白质的多核苷酸构建体。 蛋白质不同地被称为TIC2463相关毒素蛋白质类别或家族，TIC2463相关毒素蛋白，TIC2463相关蛋白属，与TIC2463毒素蛋白相关的毒素蛋白，与TIC2463相关的蛋白质，TIC2463相关毒素 多肽，TIC2463相关的杀虫蛋白等。