公开(公告)号：US20250002932A1

公开(公告)日：2025-01-02

申请号：US18830256

申请日：2024-09-10

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Jason S. Milligan ,  James K. Roberts ,  Yong Yin

IPC: C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

公开(公告)号：US20240287539A1

公开(公告)日：2024-08-29

申请号：US18426976

申请日：2024-01-30

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  A01N63/50 ,  C07K14/195

CPC classification number: C12N15/8286 ,  A01N63/50 ,  C07K14/195

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US11198888B2

公开(公告)日：2021-12-14

申请号：US16684007

申请日：2019-11-14

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/20

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US20210332380A1

公开(公告)日：2021-10-28

申请号：US17235129

申请日：2021-04-20

Applicant: Monsanto Technology LLC

Inventor： Sarah L. Brown ,  Stanislaw Flasinski ,  Aihong Pan ,  Jason W. Stelzer ,  Heidi M. Windler ,  Yong Yin

IPC: C12N15/82 ,  C12Q1/6895

Abstract: The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

公开(公告)号：US11130964B2

公开(公告)日：2021-09-28

申请号：US16205426

申请日：2018-11-30

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/50

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US20200370066A1

公开(公告)日：2020-11-26

申请号：US16895921

申请日：2020-06-08

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Jason S. Milligan ,  James K. Roberts ,  Yong Yin

IPC: C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

公开(公告)号：US10662439B2

公开(公告)日：2020-05-26

申请号：US14945140

申请日：2015-11-18

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  C07K14/195 ,  A01N63/20

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US20230332171A1

公开(公告)日：2023-10-19

申请号：US18182839

申请日：2023-03-13

Applicant: Monsanto Technology LLC

Inventor： Sarah L. Brown ,  Stanislaw Flasinski ,  Aihong Pan ,  Jason W. Stelzer ,  Heidi M. Windler ,  Yong Yin

IPC: C12N15/82 ,  C12Q1/6895

CPC classification number: C12N15/8286 ,  C12Q1/6895 ,  C12Q2600/158 ,  C12Q2600/13

Abstract: The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

公开(公告)号：US20220364109A1

公开(公告)日：2022-11-17

申请号：US17829963

申请日：2022-06-01

Applicant: Monsanto Technology LLC

Inventor： Sarah L. Brown ,  Stanislaw Flasinski ,  Aihong Pan ,  Jason W. Stelzer ,  Heidi M. Windler ,  Yong Yin

IPC: C12N15/82 ,  C12Q1/6895

Abstract: The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

公开(公告)号：US09957524B2

公开(公告)日：2018-05-01

申请号：US14671542

申请日：2015-03-27

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Uma R. Kesanapalli ,  Jason S. Milligan ,  Rachael N. Slightom ,  Daqi Tu ,  Andrew M. Wollacott ,  Yong Yin

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: The invention generally relates to the field of insect inhibitory toxin proteins. A novel class of proteins exhibiting insect inhibitory activity against agriculturally relevant pests of crop plants and seeds are disclosed. Insecticidal activity is particularly effective against the Coleopteran order of insect pests. Plants, plant parts, and seed are provided containing a polynucleotide construct encoding one or more of the toxin proteins disclosed herein. The proteins are referred to herein variously as the TIC2463-related toxin protein class or family, the TIC2463-related toxin proteins, the TIC2463-related protein genus, toxin proteins related to the TIC2463 toxin protein, proteins related to TIC2463, TIC2463-related toxin polypeptides, TIC2463-related pesticidal proteins, and the like.