公开(公告)号：US20060131036A1

公开(公告)日：2006-06-22

申请号：US11266973

申请日：2005-11-04

申请人： Scott Franson ,  Kenneth Vos ,  Thomas Winebrenner ,  Andrew Thompson

发明人： Scott Franson ,  Kenneth Vos ,  Thomas Winebrenner ,  Andrew Thompson

IPC分类号： A62C37/08

CPC分类号： A62C37/09 ,  A62C37/14

摘要： A pendant sprinkler head includes a sprinkler body, with an orifice that forms an inlet and an outlet. A deflector is movably supported by a deflector support assembly and is movable between a retracted position wherein the deflector is adjacent the outlet and an extended position where the deflector is spaced from the outlet. At least a portion of the deflector support assembly is located in the orifice when the deflector is in its retracted position.

摘要翻译： 吊顶喷头包括具有形成入口和出口的孔口的喷洒器主体。 偏转器由偏转器支撑组件可移动地支撑并且可在缩回位置之间移动，其中偏转器邻近出口和延伸位置，其中偏转器与出口间隔开。 当偏转器处于其缩回位置时，偏转器支撑组件的至少一部分位于孔中。

公开(公告)号：US20050042713A1

公开(公告)日：2005-02-24

申请号：US10479868

申请日：2002-06-07

申请人： Andrew Thompson ,  Christian Hamon ,  Thomas Neumann

发明人： Andrew Thompson ,  Christian Hamon ,  Thomas Neumann

IPC分类号： C07D495/04 ,  C07K1/113 ,  C07K1/12 ,  C12Q1/37 ,  G01N30/88 ,  G01N33/68 ,  C12P21/06 ,  C07K14/47

CPC分类号： G01N33/6848 ,  C07K1/12 ,  C07K1/128 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) optionally reducing disulphide linkages in the polypeptides, if they are present and capping free thiols in the polypeptides, if they are present; (b) contacting a sample comprising one or more polypeptides with a cleavage reagent which cleaves one or more polypeptides on the C-terminal side of a lysine residue to produce peptide fragments; (c) optionally deactivating the cleavage reagent; (d) contacting the sample with a lysine reactive agent to cap ε-amino groups; (e) removing those peptides having capped ε-amino groups; and (f) recovering the C-terminal peptides.

摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）如果它们存在，任选地还原多肽中的二硫键，如果它们存在则任选地还原多肽中的无帽硫醇; （b）使包含一种或多种多肽的样品与裂解试剂接触，所述切割试剂在赖氨酸残基的C末端侧切割一个或多个多肽以产生肽片段; （c）任选地使切割试剂失活; （d）使样品与赖氨酸反应剂接触以将ε-氨基封端; （e）去除具有封端的ε-氨基的那些肽; 和（f）回收C末端肽。

公开(公告)号：US20050042676A1

公开(公告)日：2005-02-24

申请号：US10479855

申请日：2002-06-07

申请人： Christian Hamon ,  Andrew Thompson ,  Karsten Kuhn ,  Thomas Neumann ,  Richard Joubert ,  Robert Johnstone ,  Gunier Schmidt

发明人： Christian Hamon ,  Andrew Thompson ,  Karsten Kuhn ,  Thomas Neumann ,  Richard Joubert ,  Robert Johnstone ,  Gunier Schmidt

IPC分类号： C07D213/71 ,  C07D215/36 ,  C07D239/38 ,  C07D241/18 ,  C07D251/58 ,  C07K1/107 ,  C07K1/12 ,  C12Q1/02 ,  C12Q1/37 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.

摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应试剂反应以阻断α-氨基; （c）用裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）除去具有未封端或未封闭的氨基的那些肽; 和（f）回收N-末端肽。