公开(公告)号：US07163803B2

公开(公告)日：2007-01-16

申请号：US10479843

申请日：2002-06-07

申请人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

发明人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

IPC分类号： C12Q1/37

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.

摘要翻译： 提供了表征多肽的方法，该方法包括以下步骤：（a）任选地减少多肽中的半胱氨酸二硫键形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

公开(公告)号：US20050095661A1

公开(公告)日：2005-05-05

申请号：US10479843

申请日：2002-06-07

申请人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Mohammed

发明人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Mohammed

IPC分类号： G01N27/62 ,  C07K1/12 ,  G01N30/88 ,  G01N33/483 ,  G01N33/68 ,  C12Q1/37

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of; (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.

摘要翻译： 提供一种表征多肽的方法，该方法包括以下步骤： （a）选择性地减少多肽中的半胱氨酸二硫桥以形成游离的硫醇，并且封端游离的硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

公开(公告)号：US20050048489A1

公开(公告)日：2005-03-03

申请号：US10489341

申请日：2002-09-16

申请人： Andrew Thompson ,  Christian Hamon ,  Jurgen Schafer ,  Karsten Kuhn ,  Joseph Schwarz ,  Thomas Neumann

发明人： Andrew Thompson ,  Christian Hamon ,  Jurgen Schafer ,  Karsten Kuhn ,  Joseph Schwarz ,  Thomas Neumann

IPC分类号： G01N27/62 ,  B01J20/281 ,  G01N30/04 ,  G01N30/72 ,  G01N30/88 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  H01J49/04 ,  C12Q1/68 ,  C07H21/04

CPC分类号： G01N33/6848 ,  G01N33/68 ,  G01N33/6851 ,  G01N2458/15 ,  H01J49/04

摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectromety, and wherein the mass marker moiety comprises an amino acid and the mass normalisation moiety comprises an amino acid.

摘要翻译： 提供了一组两个或更多个质量标签，该组中的每个标签包含通过具有至少一个酰胺键与质量标准化部分连接的质量标记部分，其中该组中每个标签的总质量可以是 相同或不同，组中每个标记物的质量标记物部分的质量可以相同或不同，并且其中在具有共同质量的质量标记物部分的组中的任何标签组中，每个标签具有总体质量 不同于该组中的所有其它标记，并且其中在组中具有共同聚集体的任何标签组中，每个标记具有与该组中所有其他质量标记部分的质量不同的质量标记部分，使得全部 组中的质量标记物通过质谱可以彼此区分，并且其中质量标记物部分包含氨基酸，质量标准化部分包含氨基酸。

公开(公告)号：US20050042713A1

公开(公告)日：2005-02-24

申请号：US10479868

申请日：2002-06-07

申请人： Andrew Thompson ,  Christian Hamon ,  Thomas Neumann

发明人： Andrew Thompson ,  Christian Hamon ,  Thomas Neumann

IPC分类号： C07D495/04 ,  C07K1/113 ,  C07K1/12 ,  C12Q1/37 ,  G01N30/88 ,  G01N33/68 ,  C12P21/06 ,  C07K14/47

CPC分类号： G01N33/6848 ,  C07K1/12 ,  C07K1/128 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) optionally reducing disulphide linkages in the polypeptides, if they are present and capping free thiols in the polypeptides, if they are present; (b) contacting a sample comprising one or more polypeptides with a cleavage reagent which cleaves one or more polypeptides on the C-terminal side of a lysine residue to produce peptide fragments; (c) optionally deactivating the cleavage reagent; (d) contacting the sample with a lysine reactive agent to cap ε-amino groups; (e) removing those peptides having capped ε-amino groups; and (f) recovering the C-terminal peptides.

摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）如果它们存在，任选地还原多肽中的二硫键，如果它们存在则任选地还原多肽中的无帽硫醇; （b）使包含一种或多种多肽的样品与裂解试剂接触，所述切割试剂在赖氨酸残基的C末端侧切割一个或多个多肽以产生肽片段; （c）任选地使切割试剂失活; （d）使样品与赖氨酸反应剂接触以将ε-氨基封端; （e）去除具有封端的ε-氨基的那些肽; 和（f）回收C末端肽。

公开(公告)号：US20050042676A1

公开(公告)日：2005-02-24

申请号：US10479855

申请日：2002-06-07

申请人： Christian Hamon ,  Andrew Thompson ,  Karsten Kuhn ,  Thomas Neumann ,  Richard Joubert ,  Robert Johnstone ,  Gunier Schmidt

发明人： Christian Hamon ,  Andrew Thompson ,  Karsten Kuhn ,  Thomas Neumann ,  Richard Joubert ,  Robert Johnstone ,  Gunier Schmidt

IPC分类号： C07D213/71 ,  C07D215/36 ,  C07D239/38 ,  C07D241/18 ,  C07D251/58 ,  C07K1/107 ,  C07K1/12 ,  C12Q1/02 ,  C12Q1/37 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.

摘要翻译： 提供了表征多肽或多肽群体的方法，该方法包括以下步骤：（a）将包含一种或多种多肽的样品与赖氨酸反应剂接触以将ε-氨基封端; （b）任选地使多肽样品与胺反应试剂反应以阻断α-氨基; （c）用裂解试剂消化多肽样品以产生肽片段; （d）任选地使切割试剂失活; （e）除去具有未封端或未封闭的氨基的那些肽; 和（f）回收N-末端肽。

公开(公告)号：US08697604B2

公开(公告)日：2014-04-15

申请号：US10550558

申请日：2004-03-18

申请人： Christian Hamon ,  Karsten Kuhn ,  Andrew Thompson ,  Dieter Reuschling ,  Juergen Schaefer

发明人： Christian Hamon ,  Karsten Kuhn ,  Andrew Thompson ,  Dieter Reuschling ,  Juergen Schaefer

IPC分类号： C40B40/00 ,  G01N33/68

CPC分类号： G01N33/68 ,  C07B2200/05 ,  C07C229/12 ,  C07D207/46 ,  C07D211/14 ,  C07D295/15 ,  C07D401/12 ,  C07K1/13 ,  C40B40/04 ,  C40B70/00 ,  G01N33/6848 ,  G01N2458/15 ,  Y10T436/24

摘要： Provided is a method for characterizing a molecule by mass spectrometry, which molecule comprises one or more free amino groups, which method comprises: (a) reacting one or more free amino groups in the molecule with a mass tag reagent comprising a reactive functionality capable of reacting with an amino group, and a tertiary amino group linked to the reactive functionality; and (b) characterizing the molecule by mass spectrometry.

摘要翻译： 本发明提供了一种通过质谱鉴定分子的方法，该分子包含一个或多个游离氨基，该方法包括：（a）使分子中的一个或多个游离氨基与包含能够 与氨基反应，和与反应性官能团连接的叔氨基; 和（b）通过质谱鉴定分子。

公开(公告)号：US20070023628A1

公开(公告)日：2007-02-01

申请号：US10550558

申请日：2004-03-18

申请人： Christian Hamon ,  Karsten Kuhn ,  Andrew Thompson ,  Dieter Reuschling ,  Juergen Schaefer

发明人： Christian Hamon ,  Karsten Kuhn ,  Andrew Thompson ,  Dieter Reuschling ,  Juergen Schaefer

IPC分类号： B01D59/44

CPC分类号： G01N33/68 ,  C07B2200/05 ,  C07C229/12 ,  C07D207/46 ,  C07D211/14 ,  C07D295/15 ,  C07D401/12 ,  C07K1/13 ,  C40B40/04 ,  C40B70/00 ,  G01N33/6848 ,  G01N2458/15 ,  Y10T436/24

摘要： Provided is a method for characterising a molecule by mass spectrometry, which molecule comprises one or more free amino groups, which method comprises: (a) reacting one or more free amino groups in the molecule with a mass tag reagent comprising a reactive functionality capable of reacting with an amino group, and a tertiary amino group linked to the reactive functionality; and (b) characterising the molecule by mass spectrometry.

摘要翻译： 本发明提供了一种通过质谱鉴定分子的方法，该分子包含一个或多个游离氨基，该方法包括：（a）使分子中的一个或多个游离氨基与包含能够 与氨基反应，和与反应性官能团连接的叔氨基; 和（b）通过质谱鉴定分子。

公开(公告)号：US20220294947A1

公开(公告)日：2022-09-15

申请号：US17691534

申请日：2022-03-10

申请人： Kevin Thomas ,  Andrew Thompson

发明人： Kevin Thomas ,  Andrew Thompson

IPC分类号： H04N5/225 ,  G06T7/00 ,  G02B7/00

摘要： A system for detecting foreign materials comprising an alternative light source (ALS), one or more filters operationally associated with the ALS, one or more camera lenses operationally associated with the ALS and the filters, one or more processors, a computer readable memory, and a computer readable storage medium operatively associated with a computing device operatively associated with the ALS, one or more sensors operationally associated with the computing device and the ALS, wherein the sensors detect the presence of the filter and a camera operationally associated with the lens and the computing device.

公开(公告)号：US09883819B2

公开(公告)日：2018-02-06

申请号：US13141048

申请日：2010-01-06

申请人： Marc Jensen ,  Robert Leichner ,  Patrick Beaulieu ,  Kit Yee Au-Yeung ,  Lawrence Arne ,  Mark Zdeblick ,  Andrew Thompson ,  George Savage ,  Timothy Robertson ,  Yashar Behzadi

发明人： Marc Jensen ,  Robert Leichner ,  Patrick Beaulieu ,  Kit Yee Au-Yeung ,  Lawrence Arne ,  Mark Zdeblick ,  Andrew Thompson ,  George Savage ,  Timothy Robertson ,  Yashar Behzadi

IPC分类号： A61B5/07 ,  A61B5/00 ,  G06F19/00 ,  H04B13/00 ,  A61B5/021 ,  A61B5/024 ,  A61B5/11

CPC分类号： A61B5/073 ,  A61B5/0002 ,  A61B5/021 ,  A61B5/024 ,  A61B5/1112 ,  A61B5/486 ,  A61B5/6833 ,  G06F19/00 ,  G06F19/3456 ,  G06F19/3481 ,  G16H10/60 ,  G16H20/00 ,  G16H20/10 ,  H04B13/005

摘要： Methods, devices and systems for acquiring information useful to support a patient in implementing and adhering to a medically prescribed therapy plan are provided. The therapy may incorporate biofeedback methods and/or personalized therapy aspects. A method includes steps of receiving, by a receiving device, biometric information associated with an ingestible event marker; analyzing, by a computing device having a microprocessor configured to perform a biometric information analysis, the biometric information; and determining a therapeutic recommendation at least partly on the basis of the analysis and/or integrating biofeedback techniques into patient therapy or activity. A system includes a biometric information module to receive biometric information associated with an ingestible event marker; an analysis module to analyze the biometric information; and a determination module to optionally determine and communicate a therapeutic recommendation at least partly on the basis of the analysis.

公开(公告)号：US08674825B2

公开(公告)日：2014-03-18

申请号：US12404184

申请日：2009-03-13

申请人： Mark Zdeblick ,  Andrew Thompson ,  Aleksandr Pikelny ,  Timothy Robertson

发明人： Mark Zdeblick ,  Andrew Thompson ,  Aleksandr Pikelny ,  Timothy Robertson

IPC分类号： G08B1/08

CPC分类号： A61B5/073 ,  A61B5/0028 ,  A61B5/0031 ,  A61B5/07 ,  A61B5/076 ,  A61B5/1473 ,  A61B5/4833 ,  A61B5/4839 ,  A61B5/681 ,  A61B5/6861 ,  A61B5/7282 ,  A61B2560/0214 ,  A61B2560/0462 ,  A61B2562/08 ,  A61B2562/162 ,  A61J3/007 ,  G06F19/00 ,  G06K7/10168 ,  H01Q1/273 ,  Y10T29/49117

摘要： Compositions, systems and methods that allow for the detection of the actual physical delivery of a pharmaceutical agent to a body are provided. Embodiments of the compositions include an identifier and an active agent. The invention finds use in a variety of different applications, including but not limited to, monitoring of therapeutic regimen compliance, tracking the history of pharmaceutical agents, etc.

摘要翻译： 提供了允许检测药物到身体的实际物理输送的组合物，系统和方法。 组合物的实施方案包括标识符和活性剂。 本发明可用于各种不同的应用，包括但不限于监测治疗方案依从性，跟踪药剂的历史等。