公开(公告)号：US08840877B2

公开(公告)日：2014-09-23

申请号：US12999076

申请日：2009-06-26

Applicant: Gord Adamson ,  David Bell ,  Steven Brookes

Inventor： Gord Adamson ,  David Bell ,  Steven Brookes

IPC: A61K38/17 ,  A61K38/18 ,  A61K38/19 ,  A61K38/22 ,  A61K38/36 ,  A61K38/38 ,  A61K38/43 ,  A61K38/55 ,  A61K39/395 ,  A61K47/48 ,  A61K38/44 ,  A61K38/57 ,  C07K16/28 ,  A61K38/16 ,  A61K38/50 ,  A61K38/28 ,  A61K38/21 ,  A61K38/37 ,  A61K49/00

CPC classification number: A61K47/4823 ,  A61K38/166 ,  A61K38/193 ,  A61K38/212 ,  A61K38/28 ,  A61K38/37 ,  A61K38/385 ,  A61K38/44 ,  A61K38/48 ,  A61K38/50 ,  A61K38/57 ,  A61K39/3955 ,  A61K47/60 ,  A61K47/61 ,  A61K49/0017 ,  C07K16/2896 ,  C12Y107/03003 ,  C12Y305/01001

Abstract: Method for preparing an oxidized polysaccharide-protein composition, by (a) oxidizing a polysaccharide with an oxidizing agent to form an oxidized polysaccharide where less than 20% of the oxidized units are comprised of alpha-hydroxy aldehyde units, (b) reacting the oxidized polysaccharide with a protein to form a composition comprising an oxidized polysaccharide-protein conjugate, and (c) maintaining the oxidized polysaccharide-protein conjugate composition by placing it in an environment where the temperature is less than 8° C. The oxidized polysaccharide and the protein are conjugated via one or more imine bonds, the oxidized polysaccharide-protein composition is soluble in aqueous solvent, and the composition is capable of releasing the protein.

Abstract translation: 通过（a）用氧化剂氧化多糖以形成氧化多糖的方法，其中少于20％的氧化单元由α-羟基醛单元组成，（b）使氧化的多糖 - 蛋白质组合物 多糖与蛋白质形成包含氧化多糖 - 蛋白质缀合物的组合物，和（c）通过将氧化的多糖 - 蛋白质缀合物组合物置于温度低于8℃的环境中来维持氧化多糖 - 蛋白质结合物组合物。氧化多糖和蛋白质 通过一个或多个亚胺键共轭，氧化的多糖 - 蛋白质组合物可溶于含水溶剂，组合物能释放蛋白质。

公开(公告)号：US20160009757A1

公开(公告)日：2016-01-14

申请号：US14688906

申请日：2015-04-16

Applicant: Therapure Biopharma Inc.

Inventor： Allan Otto Fog LIHME

IPC: C07K1/16 ,  C07K14/76 ,  C07K14/75 ,  B01D15/20 ,  C07K14/79 ,  B01D15/08 ,  B01D15/42 ,  C07K14/81 ,  C07K16/00

CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

Abstract: The present invention provides a process for the isolation of one or more proteins) from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific proteins) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been supplemented with an alcohol.

Abstract translation: 本发明提供了从蛋白质溶液中分离一种或多种蛋白质的方法）。 该方法包括以下步骤：a）提供包含一种或多种特定蛋白质的蛋白质溶液）并具有预设的pH和预设的离子强度或导电性，b）将蛋白质溶液施加到包含吸附剂的填充床或膨胀床柱 ，和c）从所述柱中获得一种或多种蛋白质） 其中所述蛋白质溶液已经被补充了醇。

公开(公告)号：US20130209419A1

公开(公告)日：2013-08-15

申请号：US13768830

申请日：2013-02-15

Applicant: Therapure Biopharma Inc.

Inventor： Kathryn E. Matthews ,  David N. Bell

IPC: A61K38/02

CPC classification number: A61K38/02 ,  A61K35/28 ,  A61K38/42

Abstract: Methods and compositions for improving the engraftment of stem cells are described. The method involves administering an effective amount of hemoglobin. The methods and compositions are useful in stimulating hematopoiesis and may be used to boost human hematopoietic re-engraftment for use in transplant recipients or in patients with bone marrow injury.

Abstract translation: 描述了用于改善干细胞移植的方法和组合物。 该方法包括施用有效量的血红蛋白。 所述方法和组合物可用于刺激造血作用，并且可用于促进用于移植受体或骨髓损伤患者的人造血再植入。

公开(公告)号：US20120213732A1

公开(公告)日：2012-08-23

申请号：US13399046

申请日：2012-02-17

Applicant: J. Gordon Adamson ,  David Bell ,  Nancy Ng ,  Petrus Biessels

Inventor： J. Gordon Adamson ,  David Bell ,  Nancy Ng ,  Petrus Biessels

IPC: A61K47/48 ,  A61P7/06 ,  A61P31/18 ,  C07K14/805 ,  A61P31/14

CPC classification number: A61K31/7056 ,  A61K47/62 ,  A61K47/6445

Abstract: This invention relates to targeted delivery of anti-viral compounds through protein bioconjugation. More particularly, it relates to an anti-viral compound conjugated to a protein, such as hemoglobin and to a method of treating a viral infection using said conjugate. The invention also provides a method of targeted drug delivery of an anti-viral nucleoside analogue to macrophages, cells comprising a hemoglobin receptor and to CD163 bearing cells.

Abstract translation: 本发明涉及通过蛋白质生物共轭靶向递送抗病毒化合物。 更具体地说，本发明涉及与蛋白质（例如血红蛋白）缀合的抗病毒化合物以及使用所述缀合物治疗病毒感染的方法。 本发明还提供了将抗病毒核苷类似物靶向药物递送至巨噬细胞，包含血红蛋白受体的细胞和携带CD163的细胞的方法。

公开(公告)号：US20210139534A1

公开(公告)日：2021-05-13

申请号：US16492766

申请日：2018-03-16

Applicant: THERAPURE BIOPHARMA INC.

Inventor： Jin Seog Seo ,  David Miller

IPC: C07K1/34 ,  C07K14/765

Abstract: A method for the purification of albumin from plasma is described. The method comprises (a) contacting the plasma with sodium caprylate (NaCP) at an amount dependent on total protein concentration and the ratio of NaCP to total protein in the plasma, (b) heating the plasma at a near neutral pH range, and (c) separating the albumin from non-albumin-phase. The said method provides for a high yield and purity albumin solution.

公开(公告)号：US09624260B2

公开(公告)日：2017-04-18

申请号：US11570154

申请日：2005-06-07

Applicant: Allan Otto Fog Lihme

Inventor： Allan Otto Fog Lihme

IPC: C07K1/18 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/81 ,  B01D15/08 ,  B01D15/20 ,  B01D15/42 ,  C07K14/79 ,  C07K16/00

CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

Abstract: The present invention provides a process for the isolation of one or more proteins) from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific proteins) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been supplemented with an alcohol.

公开(公告)号：US09428545B2

公开(公告)日：2016-08-30

申请号：US14688906

申请日：2015-04-16

Applicant: Therapure Biopharma Inc.

Inventor： Allan Otto Fog Lihme

IPC: C07K1/18 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/81 ,  B01D15/08 ,  B01D15/20 ,  B01D15/42 ,  C07K14/79 ,  C07K16/00

CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

Abstract: The present invention provides a process for the isolation of one or more proteins from a protein solution. This process comprising the steps of: a) providing a protein solution comprising one or more specific proteins and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been not supplemented with an alcohol.

Abstract translation: 本发明提供了从蛋白质溶液中分离一种或多种蛋白质的方法。 该方法包括以下步骤：a）提供包含一种或多种特定蛋白质并具有预设pH和预设离子强度或导电性的蛋白质溶液，b）将蛋白质溶液施用于包含吸附剂的填充床或膨胀床柱， 和c）从所述柱中获得一种或多种蛋白质） 其中所述蛋白质溶液未被补充。

公开(公告)号：US20230190947A1

公开(公告)日：2023-06-22

申请号：US16310197

申请日：2017-06-21

Applicant: Therapure Biopharma Inc.

Inventor： Steven Brookes ,  J. Gordon Adamson ,  David Bell

IPC: A61K47/64 ,  A61P35/04 ,  A61P35/00 ,  A61P1/00

CPC classification number: A61K47/6445 ,  A61P35/04 ,  A61P35/00 ,  A61P1/00

Abstract: The use of hemoglobin as a targeting carrier for drugs is disclosed. Such hemoglobin-drug complexes have utility in the treatment of cancer, in particular cancers of the liver and colon. Said drug is preferably a nucleoside analog, in particular floxuridine and is covalently attached to the hemoglobin in a molar drug ratio of between 1 and 20.

公开(公告)号：US11427613B2

公开(公告)日：2022-08-30

申请号：US16492766

申请日：2018-03-16

Applicant: THERAPURE BIOPHARMA INC.

Inventor： Jin Seog Seo ,  David Miller

IPC: C07K1/34 ,  C07K14/765

Abstract: A method for the purification of albumin from plasma is described. The method comprises (a) contacting the plasma with sodium caprylate (NaCP) at an amount dependent on total protein concentration and the ratio of NaCP to total protein in the plasma, (b) heating the plasma at a near neutral pH range, and (c) separating the albumin from non-albumin-phase. The said method provides for a high yield and purity albumin solution.

公开(公告)号：US20170282095A1

公开(公告)日：2017-10-05

申请号：US15451743

申请日：2017-03-07

Applicant: Therapure Biopharma Inc.

Inventor： Allan Otto Fog Lihme

IPC: B01D15/08 ,  B01D15/42 ,  C07K16/00 ,  C07K14/755 ,  C07K14/81 ,  B01D15/20 ,  C07K1/16

CPC classification number: B01D15/08 ,  B01D15/206 ,  B01D15/424 ,  C07K1/16 ,  C07K14/47 ,  C07K14/75 ,  C07K14/755 ,  C07K14/76 ,  C07K14/79 ,  C07K14/81 ,  C07K16/00

Abstract: The present invention provides a process for the isolation of one or more proteins) from a protein solution. The process comprising the steps of: a) providing a protein solution comprising one or more specific proteins) and having a preset pH and a preset ionic strength or conductivity, b) applying the protein solution to a packed bed or expanded bed column comprising an adsorbent, and c) obtaining one or more proteins) from the column; wherein the protein solution has been supplemented with an alcohol.