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    ISOLATION OF IMMUNOGLOBULINS
    1.
    发明申请
    ISOLATION OF IMMUNOGLOBULINS 有权
    免疫球蛋白的分离

    公开(公告)号：US20080132683A1

    公开(公告)日：2008-06-05

    申请号：US11930556

    申请日：2007-10-31

    申请人： Allan Otto Fog LIHME ,  Marie Bendix Hansen

    发明人： Allan Otto Fog LIHME ,  Marie Bendix Hansen

    IPC分类号： C07K16/00 ,  C07D211/04 ,  C07D235/28 ,  C07C63/06 ,  C07K1/22

    CPC分类号： C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

    摘要翻译： 本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。 杂交瘤细胞培养上清液，动物血浆或血清或初乳。 该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。 固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如， 羧酸。 所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

    Isolation of proteins
    2.
    发明授权
    Isolation of proteins 有权
    蛋白质的分离

    公开(公告)号：US06919436B2

    公开(公告)日：2005-07-19

    申请号：US10268667

    申请日：2002-10-11

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    IPC分类号： G01N33/53 ,  A61K39/395 ,  B01D15/00 ,  B01D15/18 ,  B01D15/38 ,  B01J20/26 ,  B01J20/32 ,  C07K1/22 ,  C07K16/00 ,  G01N33/531

    CPC分类号： C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activiated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

    摘要翻译： 本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。 杂交瘤细胞培养上清液，动物血浆或血清或初乳。 该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。 固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如。 羧酸。 所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

    System and method for producing beads
    4.
    发明授权
    System and method for producing beads 有权
    珠粒的制备方法

    公开(公告)号：US09192907B2

    公开(公告)日：2015-11-24

    申请号：US12745611

    申请日：2008-12-02

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    IPC分类号： B01J2/08 ,  B01J13/04

    CPC分类号： B01J2/08 ,  B01J13/046

    摘要： The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilizing the droplets by transferring the emulsion from the first reactor to a stabilization reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.

    摘要翻译： 本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）将（a）包含能够凝胶化的材料的液体组合物组合; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够 凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经受凝胶化手段，以在5分钟以内获得凝胶化，并形成珠粒。

    RECYCLING OF ENZYMES FROM BIOREACTORS
    7.
    发明申请
    RECYCLING OF ENZYMES FROM BIOREACTORS 审中-公开
    生物反应器回收酶

    公开(公告)号：US20150307902A1

    公开(公告)日：2015-10-29

    申请号：US13806052

    申请日：2011-06-24

    申请人： Allan Otto Fog LIHME

    发明人： Allan Otto Fog LIHME

    IPC分类号： C12P7/10 ,  C12P7/16 ,  C12P7/64 ,  C12P7/04

    CPC分类号： C12P7/10 ,  B01D15/168 ,  B01D15/1892 ,  B01D15/3809 ,  C07K1/22 ,  C12P7/04 ,  C12P7/16 ,  C12P7/649 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/17

    摘要： A method of conducting an enzymatic process in which the enzymes are recovered and reused in at least a second iteration of the process, wherein each iteration of the process comprises the steps of: (a) providing a heterogeneous substrate solution, in which the substrate is fully soluble, partially soluble or insoluble; (b) adding an enzyme or mixture of enzymes to the heterogeneous substrate solution; and (c) allowing an enzymatic reaction of the substrate to proceed; wherein, after completion of step (c) for each iteration, the enzyme is recovered from the mixture resulting from step (c) according to the following steps: (d) conducting a non-packed-bed adsorption process comprising contacting the reaction mixture with an adsorbent that adsorbs the enzyme in order to separate the enzyme from the reaction mixture resulting from step (c); (e) optionally washing unbound material from the adsorbent; and (f) desorbing the enzyme from the adsorbent; and further wherein the desorbed enzyme obtained in step (f) is used in step (b) of at least one subsequent iteration of the process.

    摘要翻译： 一种进行酶过程的方法，其中酶在该方法的至少第二次迭代中回收和重复使用，其中该方法的每次迭代包括以下步骤：（a）提供非均相底物溶液，其中所述底物为 完全溶解，部分溶解或不溶; （b）向所述非均相底物溶液中加入酶或酶的混合物; 和（c）允许底物的酶促反应进行; 其中，对于每次迭代，在步骤（c）完成后，根据以下步骤从步骤（c）得到的混合物中回收酶：（d）进行非填充床吸附方法，包括使反应混合物与 吸附酶以便将酶与步骤（c）得到的反应混合物分离的吸附剂; （e）任选地从吸附剂洗涤未结合的材料; 和（f）从吸附剂中解吸酶; 并且其中步骤（f）中获得的解吸酶在步骤（b）中用于该方法的至少一次后续迭代。

    Production of biofuel and protein from a raw material
    8.
    发明授权
    Production of biofuel and protein from a raw material 有权
    从原料生产生物燃料和蛋白质

    公开(公告)号：US08815551B2

    公开(公告)日：2014-08-26

    申请号：US12522885

    申请日：2008-01-15

    申请人： Allan Otto Fog Lihme

    发明人： Allan Otto Fog Lihme

    IPC分类号： C12P7/16 ,  A23J1/00

    CPC分类号： C10L1/1802 ,  B01D15/1807 ,  C07K1/16 ,  C12P3/00 ,  C12P5/023 ,  C12P7/02 ,  C12P7/08 ,  C12P7/16 ,  C12P7/62 ,  C12P7/649 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

    摘要： The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.

    摘要翻译： 本发明涉及从适合于生产生物燃料的原料或所述原料的衍生物提供分离的生物燃料和纯化的蛋白质产物的方法。 该方法包括以下步骤：（i）使所述原料或所述原料的衍生物经受从原料或所述原料的衍生物释放生物燃料的至少一种第一处理，（ii）分离生物燃料中释放的生物燃料 步骤（i）获得分离的生物燃料，（iii）使所述原料或所述原料的衍生物经受提供材料悬浮液的至少一次第二处理，和（iv）使来自步骤（iii）的材料悬浮液经膨胀 床吸附过程获得纯化的蛋白质产物。

    Isolation of immunoglobulins
    9.
    发明授权
    Isolation of immunoglobulins 有权
    免疫球蛋白的分离

    公开(公告)号：US07745582B2

    公开(公告)日：2010-06-29

    申请号：US11930556

    申请日：2007-10-31

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    IPC分类号： C07K1/22 ,  C07K16/00

    CPC分类号： C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

    摘要翻译： 本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。 杂交瘤细胞培养上清液，动物血浆或血清或初乳。 该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。 固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如， 羧酸。 所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。