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公开(公告)号：US20240076632A1
公开(公告)日：2024-03-07
申请号：US18461321
申请日：2023-09-05
发明人： Yoshihiro Kawaoka , Shinya Yamada , Shiho Chiba
CPC分类号： C12N7/00 , A61K39/00 , C12N2760/16021 , C12N2760/16022 , C12N2760/16034 , C12N2760/16051
摘要： Modified influenza virus neuraminidases are described herein that improve viral replication, thus improving the yield of vaccine viruses. Expression of such modified neuraminidases by influenza virus may also stabilize co-expressed hemagglutinins so that the hemagglutinins do not undergo mutation or decrease the need for HA binding to cells.
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公开(公告)号：US20230369945A1
公开(公告)日：2023-11-16
申请号：US17743707
申请日：2022-05-13
发明人： Bulent Sarlioglu , Gokhan Cakal
摘要： Stator packages and electric machines, and methods for cooling same, are disclosed herein. In an example embodiment, an electric machine includes a rotor, and a stator package having one or more stator coils, stator core portions, and a heat exchanger. The stator core portions and heat exchanger are arranged successively along a central axis, with the heat exchanger being positioned between the stator core portions. Each of the stator coils is arranged to extend along/within the stator core portions and heat exchanger. The heat exchanger includes walls forming passageways configured so that coolant entering the heat exchanger via an inlet both flows circumferentially from the inlet to an outlet, and additionally flows in an undulating manner both radially inwardly and outwardly substantially in between outer and inner wall surfaces of the heat exchanger, such that the coolant cools the one or more stator coils and the stator core portions.
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3.发明公开公开(公告)号：US20230190913A1
公开(公告)日：2023-06-22
申请号：US17813178
申请日：2022-07-18
发明人： Yoshihiro Kawaoka , Gabriele Neumann , Huihui Kong
IPC分类号： A61K39/145 , C07K16/10 , C07K16/34 , C12N15/86
CPC分类号： A61K39/145 , C07K16/1018 , C07K16/34 , C12N15/86 , A61K2039/521
摘要： Methods of preparing influenza viruses having altered immunodominant epitopes in HA, e.g., having one or more residues in one or more of antigenic sites A-E in HA altered, and viral vectors, e.g., influenza virus VLPs or non-influenza viruses or VLPs thereof expressing or having influenza HAs with altered immunogenicity as a result of altered immunodominant epitopes therein are provided.
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公开(公告)号：US20220202926A1
公开(公告)日：2022-06-30
申请号：US17546835
申请日：2021-12-09
发明人： Yoshihiro Kawaoka , Gabriele Neumann , Jihui Ping
IPC分类号： A61K39/145 , C12N7/00 , A61K39/12 , C12N15/86
摘要： The invention provides a composition useful to prepare high titer influenza B viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza B virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.
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公开(公告)号：US20210252130A1
公开(公告)日：2021-08-19
申请号：US17229001
申请日：2021-04-13
摘要： The invention provides a vaccine comprising an effective amount of an isolated recombinant influenza virus comprising a mutant M gene segment that is mutated so that upon viral replication the mutant M gene expresses a functional M1 protein and a mutant M2 protein with a deletion of the cytoplasmic tail and either lacking a transmembrane domain or having a mutated transmembrane domain.
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公开(公告)号：US10246686B2
公开(公告)日：2019-04-02
申请号：US15865364
申请日：2018-01-09
发明人： Yoshihiro Kawaoka , Gabriele Neumann , Jihui Ping
IPC分类号： A61K39/145 , A61K39/00 , A61K39/12 , C12N7/00 , C07K14/005 , C12N15/86
摘要： The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.
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7.发明授权公开(公告)号：US10172934B2
公开(公告)日：2019-01-08
申请号：US15593039
申请日：2017-05-11
发明人： Yoshihiro Kawaoka , Gabriele Neumann , Jihui Ping
IPC分类号： A61K39/145 , A61K39/00 , C12N7/00 , A61K39/12 , C07K14/005
摘要： The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.
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公开(公告)号：US10119124B2
公开(公告)日：2018-11-06
申请号：US15292595
申请日：2016-10-13
发明人： Tokiko Watanabe , Yoshihiro Kawaoka
IPC分类号： C12N7/00 , A61K39/145 , A61K39/00
摘要： A method to prepare recombinant influenza viruses comprising a mutant M2 protein which has a deletion of two or more residues in the cytoplasmic tail and is attenuated in vivo, is provided, as well the resulting virus and vaccines with the virus.
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公开(公告)号：US20070148674A1
公开(公告)日：2007-06-28
申请号：US11615695
申请日：2006-12-22
申请人： Mark BERRES , David Frisch , Fredrick Blattner
发明人： Mark BERRES , David Frisch , Fredrick Blattner
CPC分类号： G01N33/5308 , C12Q1/6816 , C12Q1/6837 , C12Q1/6869 , C12Q2565/601 , C12Q2565/513 , C12Q2522/101
摘要： The present invention provides methods and apparatus for the optical fingerprinting of nucleic acid molecules. In certain embodiments, the invention provides methods for labeling nucleic acid molecules using-site-specific nucleic acid binding partners that bind to nucleic acids without cleaving the molecule. The nucleic acid binding partners can be labeled directly with a fluorophore, such as a quantum dot (QD), or indirectly. Examples of suitable binding partners include cut-deficient restriction endonucleases (cdREs), transcription factors, the binding domains of nucleic acid polymerases, antibodies and the like. The methods disclosed make the assembly of fingerprint contigs easier and allows for digitizing the results so as to provide for easier computer manipulation and assembly. The invention also includes a microarray, the microarray designed to provide for easy deposition and high-throughput fingerprinting. The invention also provides for methods of using the microarrays. The invention also provides kits for the use of the invention.
摘要翻译： 本发明提供了核酸分子的光学指纹图谱的方法和装置。 在某些实施方案中，本发明提供了使用与核酸结合但不裂解该分子的位点特异性核酸结合配偶体标记核酸分子的方法。 核酸结合配偶体可直接用荧光团（例如量子点（QD））或间接地标记。 合适的结合配偶体的实例包括切断缺陷型限制性内切核酸酶（cdRE），转录因子，核酸聚合酶的结合结构域，抗体等。 所公开的方法使得指纹重叠群的组装更容易，并允许数字化结果，从而提供更容易的计算机操纵和组装。 本发明还包括微阵列，微阵列被设计成提供易于沉积和高通量指纹。 本发明还提供了使用微阵列的方法。 本发明还提供了用于本发明的试剂盒。
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公开(公告)号：US20020173637A1
公开(公告)日：2002-11-21
申请号：US09847010
申请日：2001-05-01
发明人： Perry A. Frey , Frank J. Ruzicka
IPC分类号： C07H021/04
摘要： Purified null-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure null-amino acids, such as L-null-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-null-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-null-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-null-lysine in vitro.
摘要翻译： 纯化的β-氨基酸在药理活性化合物的制备中具有相当大的兴趣。 尽管通过标准化学合成可以生产对映异构体纯的β-氨基酸，例如L-β-赖氨酸，但是这种传统方法是耗时的，需要昂贵的起始原料，并且导致必须进一步纯化的外消旋混合物。 然而，编码赖氨酸2,3-氨基变位酶的DNA分子可用于通过避免化学合成缺陷的方法制备L-β-赖氨酸。 特别地，L-β-赖氨酸可以通过表达重组赖氨酸2,3-氨基变位酶的宿主细胞的培养物合成。 或者，这样的重组宿主细胞可提供用于分离赖氨酸2,3-氨基变位酶的来源，其依次可用于在体外产生L-β-赖氨酸。
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