公开(公告)号：US20220371015A1

公开(公告)日：2022-11-24

申请号：US17747854

申请日：2022-05-18

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： B01L3/00 ,  B01L9/00 ,  G01N27/327 ,  G01N33/543

摘要： A lateral flow assay device comprises a test strip to receive a quantity of fluid comprising a quantity of exosomes and detect the presence of a target analyte on the surface of the exosomes. The test strip comprises a conjugate pad that contains a set of one or more types of tetraspanin binding reagents conjugated with a label. Each type of tetraspanin binding reagent is configured to bind with a corresponding type of exosome tetraspanin and form an immunocomplex comprising an exosome. The conjugate pad is fluidly connected to a membrane. The membrane comprises a test line comprising an immobilized binding reagent to the target analyte. The immobilized binding reagent to the target analyte is configured to bind to a protein of the target analyte on the surface of an exosome in an immunocomplex comprising the exosome.

公开(公告)号：US11435374B2

公开(公告)日：2022-09-06

申请号：US17521853

申请日：2021-11-08

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N35/10 ,  G01N33/49 ,  G01N35/00

摘要： A sample collection and testing device for analyzing blood is provided that includes a controller, a fluid flow pathway, a pump configured to move fluid through the fluid pathway, and an optical fluid measurement element configured to measure a light intensity of the fluid in the fluid flow pathway. The controller is configured to: start the pump to move a blood sample in the fluid flow pathway, receive a signal from the optical fluid measurement element indicating a detection of a leading edge of the blood in the fluid flow pathway, stop the pump to stop the moving of the blood in the pathway, receive a plurality of light intensity measurements from the optical measurement element, each light intensity measurement measured at a corresponding point of time, and provide a mapping of the light intensity measurements into an indication of a coagulation of the blood sample over a time period.

公开(公告)号：US11307164B2

公开(公告)日：2022-04-19

申请号：US17332989

申请日：2021-05-27

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N27/327 ,  B01L3/00 ,  G01N33/53

摘要： A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid, and a membrane comprising a test line for determining whether the fluid comprises a target analyte. The device includes an actuator connected to a moving element comprising a shaft or a wheel. A first magnet is connected to the backing of the membrane. Second and third magnets are connected to the moving element. The actuator moves the moving element between first and second positions. In the first position, the opposite poles of the first and second magnets attract each other and maintain a gap between the membrane and the conjugate pad separate. In the second position, the similar poles of the first and third magnets repel each other and close the gap between membrane and the conjugate pad, allowing the fluid to flow from the conjugate pad into the membrane and the test line.

公开(公告)号：US20220095971A1

公开(公告)日：2022-03-31

申请号：US17548514

申请日：2021-12-11

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： A61B5/15

摘要： A test cartridge includes an adjustable lancet. The adjustable lancet is controlled by a controller. The adjustable lancet automatically detects a subject's finger, adjusts the lancet's height, pricks the finger to draw blood, moves a tube to collect the blood, moves the tube away from the finger, and empties the blood from the tube into a vial or receptacle. The adjustable lancet may include safety features to prevent the lancet to trigger when the subject's fingernail is facing the lancet, to control the amount that the lancet pierces the subject's finger, and/or to prevent the reuse of a test cartridge for multiple persons or multiple times by the same person. The adjustable lancet may include a massager wheel and/or a pressure bar to rub the subject's finger after the finger is pierced to facilitate drawing of the blood from the finger.

公开(公告)号：US11493509B2

公开(公告)日：2022-11-08

申请号：US17232553

申请日：2021-04-16

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N33/543

摘要： A lateral flow assay device includes a test strip that is configured to receive a sample fluid and detect a presence of antibodies to one or more of a plurality of proteins of the target pathogen. The lateral flow assay device includes a conjugate pad and a membrane. The conjugate pad contains a plurality of the proteins of the target pathogen, each conjugated with a label. If the sample fluid contains antibodies that are specific to the target pathogen through any of the target pathogen's proteins, a binding takes place between those antibodies and the corresponding tagged protein. The membrane may include a plurality of test lines. Each test line may contain the immobilized binding reagent to one antibody class, resulting in the concentration of all the molecules of that antibody class on the test line.

公开(公告)号：US20220229009A1

公开(公告)日：2022-07-21

申请号：US17715415

申请日：2022-04-07

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N27/327 ,  B01L3/00 ,  G01N33/53

摘要： A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid, and a membrane comprising a test line for determining whether the fluid comprises a target analyte. The device includes an actuator connected to a moving element comprising a shaft or a wheel. A first magnet is connected to the backing of the membrane. Second and third magnets are connected to the moving element. The actuator moves the moving element between first and second positions. In the first position, the opposite poles of the first and second magnets attract each other and maintain a gap between the membrane and the conjugate pad separate. In the second position, the similar poles of the first and third magnets repel each other and close the gap between membrane and the conjugate pad, allowing the fluid to flow from the conjugate pad into the membrane and the test line.

公开(公告)号：US20220065889A1

公开(公告)日：2022-03-03

申请号：US17521853

申请日：2021-11-08

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N35/10 ,  G01N33/49 ,  G01N35/00

摘要： A sample collection and testing device for analyzing blood is provided that includes a controller, a fluid flow pathway, a pump configured to move fluid through the fluid pathway, and an optical fluid measurement element configured to measure a light intensity of the fluid in the fluid flow pathway. The controller is configured to: start the pump to move a blood sample in the fluid flow pathway, receive a signal from the optical fluid measurement element indicating a detection of a leading edge of the blood in the fluid flow pathway, stop the pump to stop the moving of the blood in the pathway, receive a plurality of light intensity measurements from the optical measurement element, each light intensity measurement measured at a corresponding point of time, and provide a mapping of the light intensity measurements into an indication of a coagulation of the blood sample over a time period.

公开(公告)号：US11202593B2

公开(公告)日：2021-12-21

申请号：US16663711

申请日：2019-10-25

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： A61B5/15

摘要： A test cartridge includes an adjustable lancet. The adjustable lancet is controlled by a controller. The adjustable lancet automatically detects a subject's finger, adjusts the lancet's height, pricks the finger to draw blood, moves a tube to collect the blood, moves the tube away from the finger, and empties the blood from the tube into a vial or receptacle. The adjustable lancet may include safety features to prevent the lancet to trigger when the subject's fingernail is facing the lancet, to control the amount that the lancet pierces the subject's finger, and/or to prevent the reuse of a test cartridge for multiple persons or multiple times by the same person. The adjustable lancet may include a massager wheel and/or a pressure bar to rub the subject's finger after the finger is pierced to facilitate drawing of the blood from the finger.

公开(公告)号：US20210285910A1

公开(公告)日：2021-09-16

申请号：US17332989

申请日：2021-05-27

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N27/327 ,  B01L3/00 ,  G01N33/53

摘要： A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid, and a membrane comprising a test line for determining whether the fluid comprises a target analyte. The device includes an actuator connected to a moving element comprising a shaft or a wheel. A first magnet is connected to the backing of the membrane. Second and third magnets are connected to the moving element. The actuator moves the moving element between first and second positions. In the first position, the opposite poles of the first and second magnets attract each other and maintain a gap between the membrane and the conjugate pad separate. In the second position, the similar poles of the first and third magnets repel each other and close the gap between membrane and the conjugate pad, allowing the fluid to flow from the conjugate pad into the membrane and the test line.

公开(公告)号：US20200166473A1

公开(公告)日：2020-05-28

申请号：US16698788

申请日：2019-11-27

申请人： 2Pi-Sigma Corp.

发明人： Mehdi Hatamian

IPC分类号： G01N27/327 ,  G01N33/53 ,  B01L3/00

摘要： A lateral flow assay device comprising a conjugate pad for receiving a quantity of fluid; and a membrane comprising a test line for determining whether the fluid comprises a target analyte. In a first state of the lateral flow assay device, the lateral flow assay device is configured with a removable gap between the conjugate pad and the membrane which is substantially filled with air and prevents the fluid from flowing from the conjugate pad into the membrane. In a second state of the lateral flow assay device, the removable gap is removed from between the conjugate pad and the membrane causing the conjugate pad to come in contact with the membrane, allowing the fluid to flow from the conjugate pad into the membrane and the test line by capillary action.