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公开(公告)号：US20220354132A1
公开(公告)日：2022-11-10
申请号：US17637973
申请日：2020-08-27
申请人： AB Enzymes GmbH
IPC分类号： A21D8/04
摘要： The invention relates to the use of a composition comprising an effective amount of at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives for improving at least one property of a dough comprising rye flour and/or of a bakery product prepared from said dough as well as to a method of preparing a bakery product based on rye having at least one improved property, said method comprising (i) providing a dough that has been added an effective amount of at least one polypeptide having GH12 cellulase activity or a composition comprising at least one polypeptide having GH12 cellulase activity optionally together with conventionally used baking additives, and rye flour, (ii) baking the dough for a time and temperature sufficient to yield the bakery product as well as to a baking enzyme composition for preparing rye-based bakery products comprising a polypeptide having a GH12 cellulase activity optionally together with one or more dough(s) or bakery product additives.
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公开(公告)号：US09920384B2
公开(公告)日：2018-03-20
申请号：US14758238
申请日：2013-12-23
申请人： AB Enzymes GmbH
CPC分类号： C12Q1/6895 , C07K14/37 , C12N1/14 , C12N15/80 , C12Q2600/156 , C12R1/885
摘要： The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains of Trichoderma reesei QM6a or strains derived thereof comprising the steps of a) providing a first strain being a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a Trichoderma reesei (Hypocrea jecorina) strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the first Trichoderma reesei QM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing with Trichoderma reesei QM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a Trichoderma reesei (Hypocrea jecorina) having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the genome sequences of the first strain of a) whereby said gene(s)/genetic element(s) may be fully or partially missing or existing in a mutated form or in a form having deletions or insertions in the first strain thus being a gene or a genetic element directly or indirectly associated with mating impairment in strains of Trichoderma reesei QM6a or a strain derived thereof as well as to a process for correcting the mating impairment of a Trichoderma reesei QM6a strain or a strain derived thereof having a MAT1-1 locus and that is not competent to mate with a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, wherein one or more mutated or fully or partially missing gene(s) and/or genetic element(s) identified as above is/are replaced by or complemented with the corresponding functional gene(s) and/or genetic element(s). Moreover, the invention relates to the use of a thus obtained fungal strain of the genus Trichoderma in industrial breeding and production of a product of interest. Moreover, the invention relates to the genes associated with mating impairment of Trichoderma reesei QM6a and strains derived therefrom and to genes essential for mating of Trichoderma reesei QM6a and strains derived therefrom.
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公开(公告)号：US09353357B2
公开(公告)日：2016-05-31
申请号：US13846155
申请日：2013-03-18
申请人： AB Enzymes GmbH
发明人： Khanh Q. Nguyen , Bruno Winter
CPC分类号： C12N9/16 , A23K10/14 , A23L29/06 , C12Y301/03026
摘要： The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.
摘要翻译： 本发明涉及编码具有植酸酶活性和增加的温度稳定性以及增加的酶活性的蛋白水解稳定性的多肽的重组DNA分子。 DNA序列是通过变异成熟的野生型大肠杆菌肌醇六磷酸酶序列得到的，其具有与野生型序列相比修饰的具有限定的氨基酸位置或与分别具有N-和/或C-末端延伸的序列 。 本发明还涉及用于表达重组植酸酶的方法及其在食物和动物饲料技术中的应用。
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公开(公告)号：US09322003B2
公开(公告)日：2016-04-26
申请号：US14692230
申请日：2015-04-21
申请人： AB Enzymes GMBH
发明人： Khanh Quoc Nguyen , Kornelia Titze , Tatiana Schwarz , Silvia Paladino , Volker Marschner , Patrick Lorenz
CPC分类号： C12N9/18 , A23D9/00 , A23D9/02 , A23D9/04 , A23J7/00 , A23K20/189 , A23V2002/00 , C11B3/00 , C11B3/003 , C12N9/16 , C12Y301/01004 , C12Y301/01005 , C12Y301/01032
摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).
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公开(公告)号：US20150299680A1
公开(公告)日：2015-10-22
申请号：US14692230
申请日：2015-04-21
申请人： AB Enzymes GMBH
发明人： Khanh Quoc Nguyen , Kornelia Titze , Tatania Schwarz , Silvia Paladino , Volker Marschner , Patrick Lorenz
CPC分类号： C12N9/18 , A23D9/00 , A23D9/02 , A23D9/04 , A23J7/00 , A23K20/189 , A23V2002/00 , C11B3/00 , C11B3/003 , C12N9/16 , C12Y301/01004 , C12Y301/01005 , C12Y301/01032
摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).
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公开(公告)号：US20150361510A1
公开(公告)日：2015-12-17
申请号：US14758238
申请日：2013-12-23
申请人： AB ENZYMES GMBH
CPC分类号： C12Q1/6895 , C07K14/37 , C12N1/14 , C12N15/80 , C12Q2600/156 , C12R1/885
摘要： The invention relates to a process for identifying gene(s)/genetic element(s) associated with mating impairment in strains of Trichoderma reesei QM6a or strains derived thereof comprising the steps of a) providing a first strain being a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, b) sexually crossing said strain with a second strain being a mating competent strain of a Trichoderma reesei (Hypocrea jecorina) strain having a complementary locus, i.e. the MAT1-1 locus, c) repeatedly back-crossing the MAT1-1 progenies from the crossing of b) or the back-crossing thereof with the first strain of a), until a strain is obtained that is substantially identical to the first Trichoderma reesei QM6a strain or a strain derived thereof, but carries the MAT1-1 locus and is mating competent for crossing with Trichoderma reesei QM6a or any of its MAT1-2 progeny, d) selecting the progeny from step c) that is mating competent for crossing with a Trichoderma reesei (Hypocrea jecorina) having a MAT1-2 locus, and e) identifying the gene(s)/genetic element(s) associated with mating impairment by comparing the genome of the progenies selected in step d) with the genome sequences of the first strain of a) whereby said gene(s)/genetic element(s) may be fully or partially missing or existing in a mutated form or in a form having deletions or insertions in the first strain thus being a gene or a genetic element directly or indirectly associated with mating impairment in strains of Trichoderma reesei QM6a or a strain derived thereof as well as to a process for correcting the mating impairment of a Trichoderma reesei QM6a strain or a strain derived thereof having a MAT1-1 locus and that is not competent to mate with a Trichoderma reesei QM6a strain having a MAT1-2 locus or a strain derived thereof, wherein one or more mutated or fully or partially missing gene(s) and/or genetic element(s) identified as above is/are replaced by or complemented with the corresponding functional gene(s) and/or genetic element(s). Moreover, the invention relates to the use of a thus obtained fungal strain of the genus Trichoderma in industrial breeding and production of a product of interest. Moreover, the invention relates to the genes associated with mating impairment of Trichoderma reesei QM6a and strains derived therefrom and to genes essential for mating of Trichoderma reesei QM6a and strains derived therefrom.
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7.发明申请POLYPEPTIDE HAVING PHYTASE ACTIVITY AND INCREASED TEMPERATURE RESISTANCE OF THE ENZYME ACTIVITY, AND NUCLEOTIDE SEQUENCE CODING SAID POLYPEPTIDE 审中-公开具有酶活性的多肽和酶活性的增加的耐温度和核苷酸序列编码所述的多肽公开(公告)号：US20140242249A1
公开(公告)日：2014-08-28
申请号：US13846155
申请日：2013-03-18
申请人： AB Enzymes GmbH
发明人： Khanh Q. Nguyen , Bruno Winter
CPC分类号： C12N9/16 , A23K10/14 , A23L29/06 , C12Y301/03026
摘要： The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.
摘要翻译： 本发明涉及编码具有植酸酶活性和增加的温度稳定性以及增加的酶活性的蛋白水解稳定性的多肽的重组DNA分子。 DNA序列是通过变异成熟的野生型大肠杆菌肌醇六磷酸酶序列得到的，其具有与野生型序列相比修饰的具有限定的氨基酸位置或与分别具有N-和/或C-末端延伸的序列 。 本发明还涉及用于表达重组植酸酶的方法及其在食物和动物饲料技术中的应用。
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