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    NOVEL EXPRESSION VECTORS AND USES THEREOF
    1.
    发明申请
    NOVEL EXPRESSION VECTORS AND USES THEREOF 审中-公开
    新兴表达载体及其用途

    公开(公告)号：US20090252707A1

    公开(公告)日：2009-10-08

    申请号：US12360615

    申请日：2009-01-27

    Applicant: KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    Inventor： KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    IPC: A61K48/00 ,  A61K35/76 ,  A61K31/713 ,  A61P31/18

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: A method for treating an HIV disease in a subject in need of said treatment, comprising administering to the subject a therapeutically effective amount of a DNA vaccine comprising an expression vector and a pharmaceutically acceptable excipient, where the expression vector comprises: (a) a heterologous promoter operatively linked to a DNA sequence encoding a nuclear-anchoring protein, where the nuclear-anchoring protein comprises: (i) a DNA binding domain which binds to a specific DNA binding sequence, and (ii) a functional domain of the Bovine Papilloma Virus Type 1 E2 protein, where the functional domain binds to a nuclear component; (b) a multimerized DNA sequence that forms a binding site for the nuclear anchoring protein; and (c) at least one expression cassette comprising a DNA sequence encoding a protein or peptide that stimulates an immune response specific to the protein or peptide; where the expression vector lacks an origin of replication functional in mammalian cells.

    Abstract translation: 一种用于治疗需要所述治疗的受试者中的HIV疾病的方法，包括给予受试者治疗有效量的包含表达载体和药学上可接受的赋形剂的DNA疫苗，其中所述表达载体包含：（a）异源 启动子可操作地连接到编码核锚定蛋白的DNA序列，其中核 - 锚定蛋白包含：（i）结合特异性DNA结合序列的DNA结合结构域，和（ii）牛乳头状瘤病毒的功能结构域 1型E2蛋白，其中功能结构域结合核成分; （b）形成核锚定蛋白的结合位点的多聚化DNA序列; 和（c）至少一个表达盒，其包含编码蛋白质或肽的DNA序列，所述DNA序列刺激对所述蛋白质或肽特异性的免疫应答; 其中表达载体缺乏在哺乳动物细胞中起作用的复制起点。

    Selection system containing non-antibiotic resistance selection marker
    3.
    发明申请
    Selection system containing non-antibiotic resistance selection marker 有权
    选择系统含有非抗生素抗性选择标记

    公开(公告)号：US20070036822A1

    公开(公告)日：2007-02-15

    申请号：US10531870

    申请日：2004-09-15

    Applicant: Tanel Tenson ,  Silja Laht ,  Maarja Ado-Jaan ,  Andres Mannik ,  Urve Toots ,  Mart Ustav

    Inventor： Tanel Tenson ,  Silja Laht ,  Maarja Ado-Jaan ,  Andres Mannik ,  Urve Toots ,  Mart Ustav

    IPC: A61K39/02 ,  C12N1/21 ,  C12N15/74

    CPC classification number: C12N9/90 ,  A61K2039/53

    Abstract: A selection system free of antibiotic resistance genes, which is based on the use of an araD gene as a selection marker carried on a vector which is inserted in a bacterial strain deficient of the araD gene. The araD gene from E. coli encodes the L-ribulose-5-phosphate-4-epimerase. A method of selecting the cells transformed with a plasmid, which contains the araD gene. The non-antibiotic selection marker makes the system suitable for producing therapeutics. The araD gene is not essential for growth of the host but manipulation of it affects the growth under certain selective conditions. Deletion of araD leads to accumulation of substance which is toxic to the host but not to humans. The araD gene is relatively small and therefore a small plasmid may be constructed, which requires less energy for replication, and leads to increased growth rate and yield.

    Abstract translation: 一种没有抗生素抗性基因的选择系统，其基于使用araD基因作为选择标记，其携带在插入araD基因缺陷的细菌菌株中的载体上。 来自大肠杆菌的araD基因编码L-核酮糖-5-磷酸-4-差向异构酶。 选择用含有araD基因的质粒转化的细胞的方法。 非抗生素选择标记使该系统适合于生产治疗。 araD基因对于宿主的生长不是必需的，但是它的操作影响在某些选择性条件下的生长。 删除araD会导致对宿主有毒的物质的积累，但不会对人体产生毒性。 araD基因相对较小，因此可以构建小质粒，其需要较少的复制能量，并且导致增加的生长速率和产率。

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