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    NOVEL EXPRESSION VECTORS AND USES THEREOF
    1.
    发明申请
    NOVEL EXPRESSION VECTORS AND USES THEREOF 审中-公开
    新兴表达载体及其用途

    公开(公告)号：US20090252707A1

    公开(公告)日：2009-10-08

    申请号：US12360615

    申请日：2009-01-27

    Applicant: KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    Inventor： KAI KROHN ,  VESNA BLAZEVIC ,  MARJA TAHTINEN ,  MART USTAV ,  URVE TOOTS ,  ANDRES MANNIK ,  ANNAMARI RANKI ,  ENE USTAV

    IPC: A61K48/00 ,  A61K35/76 ,  A61K31/713 ,  A61P31/18

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: A method for treating an HIV disease in a subject in need of said treatment, comprising administering to the subject a therapeutically effective amount of a DNA vaccine comprising an expression vector and a pharmaceutically acceptable excipient, where the expression vector comprises: (a) a heterologous promoter operatively linked to a DNA sequence encoding a nuclear-anchoring protein, where the nuclear-anchoring protein comprises: (i) a DNA binding domain which binds to a specific DNA binding sequence, and (ii) a functional domain of the Bovine Papilloma Virus Type 1 E2 protein, where the functional domain binds to a nuclear component; (b) a multimerized DNA sequence that forms a binding site for the nuclear anchoring protein; and (c) at least one expression cassette comprising a DNA sequence encoding a protein or peptide that stimulates an immune response specific to the protein or peptide; where the expression vector lacks an origin of replication functional in mammalian cells.

    Abstract translation: 一种用于治疗需要所述治疗的受试者中的HIV疾病的方法，包括给予受试者治疗有效量的包含表达载体和药学上可接受的赋形剂的DNA疫苗，其中所述表达载体包含：（a）异源 启动子可操作地连接到编码核锚定蛋白的DNA序列，其中核 - 锚定蛋白包含：（i）结合特异性DNA结合序列的DNA结合结构域，和（ii）牛乳头状瘤病毒的功能结构域 1型E2蛋白，其中功能结构域结合核成分; （b）形成核锚定蛋白的结合位点的多聚化DNA序列; 和（c）至少一个表达盒，其包含编码蛋白质或肽的DNA序列，所述DNA序列刺激对所述蛋白质或肽特异性的免疫应答; 其中表达载体缺乏在哺乳动物细胞中起作用的复制起点。

    Expression vectors and uses thereof
    3.
    发明授权
    Expression vectors and uses thereof 有权
    表达载体及其用途

    公开(公告)号：US07498314B2

    公开(公告)日：2009-03-03

    申请号：US10138098

    申请日：2002-05-03

    Applicant: Kai Krohn ,  Vesna Blazevic ,  Marja Tähtinen ,  Mart Ustav ,  Urve Toots ,  Andres Männik ,  Annamari Ranki ,  Ene Ustav

    Inventor： Kai Krohn ,  Vesna Blazevic ,  Marja Tähtinen ,  Mart Ustav ,  Urve Toots ,  Andres Männik ,  Annamari Ranki ,  Ene Ustav

    IPC: A61K48/00 ,  A61K31/711 ,  C07H21/04 ,  C12N5/10 ,  C12N1/21 ,  C12P21/00

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: The present invention relates to novel vectors, to DNA vaccines and gene therapeutics containing said vectors, to methods for the preparation of the vectors and DNA vaccines and gene therapeutics, and to therapeutic uses of said vectors. More specifically, the present invention relates to novel vectors comprising an expression cassette of a gene of a nuclear-anchoring protein, which contains a DNA binding domain capable of binding to a specific DNA sequence and a functional domain capable of binding to a nuclear component and a multimerized DNA sequence forming a binding site for the nuclear-anchoring protein, and optionally an expression cassette of a gene, genes or a DNA sequence or DNA sequences of interest. The present invention further relates to DNA vaccines and gene therapeutics containing the novel vectors, to methods for the preparation of the novel vectors and the DNA vaccines and gene therapeutics.

    Abstract translation: 本发明涉及新型载体，DNA疫苗和含有所述载体的基因治疗剂，用于制备载体和DNA疫苗和基因治疗剂的方法以及所述载体的治疗用途。 更具体地，本发明涉及包含核锚定蛋白的基因的表达盒的新载体，其包含能够结合特定DNA序列的DNA结合结构域和能够结合核成分的功能结构域， 形成核 - 锚定蛋白的结合位点的多聚DNA序列，以及任选的基因的表达盒，感兴趣的DNA序列或DNA序列。 本发明还涉及含有新载体的DNA疫苗和基因治疗剂，用于制备新载体和DNA疫苗和基因治疗剂的方法。

    METHODS AND USES INVOLVING GENETIC ABERRATIONS OF NAV3 AND ABERRANT EXPRESSION OF MULTIPLE GENES
    4.
    发明申请
    METHODS AND USES INVOLVING GENETIC ABERRATIONS OF NAV3 AND ABERRANT EXPRESSION OF MULTIPLE GENES 审中-公开
    涉及NAV3遗传异常和多发性基因表达的方法与用途

    公开(公告)号：US20120058108A1

    公开(公告)日：2012-03-08

    申请号：US13124761

    申请日：2009-10-19

    Applicant: Kai Krohn ,  Annamari Ranki ,  Emilia Carlsson ,  Kristian Ovaska ,  Valtteri Häyry ,  Sampsa Hautaniemi

    Inventor： Kai Krohn ,  Annamari Ranki ,  Emilia Carlsson ,  Kristian Ovaska ,  Valtteri Häyry ,  Sampsa Hautaniemi

    IPC: A61K39/395 ,  C40B30/04 ,  A61P35/00 ,  C12Q1/68 ,  A61K48/00

    CPC classification number: C12Q1/6886 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2320/30 ,  C12Q1/6841 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/57496 ,  G01N2333/4703 ,  G01N2333/7155

    Abstract: The present invention relates to the fields of genetics and oncology and provides methods for detecting tumors as well as methods for treating patients and predicting the prognosis to a patient. Specifically, the present invention relates to a method of demonstrating the malignant character of a tumor or cell subpopulation in a subject, to a method of predicting a prognosis, to a method of treating a subject having a tumor with NAV3 copy number change and with over expression of at least one gene or gene product selected from specific lists, and to a method of selecting a treatment to a subject. The present invention also relates to uses of NAV3 gene or gene product and at least one gene and/or gene product selected from specific lists for demonstrating the malignant character of a tumor or cell sub-population, for predicting a prognosis to a subject, for selecting a treatment to a subject, and for cancer therapy in a subject having a tumor with NAV3 copy number change. Furthermore, the present invention also relates to a use of an antagonist, antibody or inhibitory molecule of at least one gene and/or gene product selected from specific lists for cancer therapy in a subject. Still, the present invention relates to a diagnostic kit comprising tools for detecting NAV3 copy number change in a biological sample and tools for detecting over expression of at least one gene or gene product selected from specific lists in a biological sample. The present invention also relates to a use of a diagnostic kit of the invention for demonstrating the malignant character of a tumor or cell subpopulation, for predicting a prognosis to a subject with a colorectal tumor, brain tumor or tumor of epidermal keratinocytes and for selecting a treatment to a subject with a colorectal tumor, brain tumor or tumor of epidermal keratinocytes.

    Abstract translation: 本发明涉及遗传学和肿瘤学领域，并提供了用于检测肿瘤的方法以及用于治疗患者并预测患者的预后的方法。 具体地说，本发明涉及一种证实受试者的肿瘤或细胞亚群的恶性特征，预测预后的方法的方法，涉及一种用NAV3拷贝数变化治疗患有肿瘤的受试者的方法 表达选自特定列表的至少一种基因或基因产物，以及选择对受试者的治疗方法。 本发明还涉及NAV3基因或基因产物和选自特异性列表的至少一种基因和/或基因产物用于证实肿瘤或细胞亚种群的恶性特征，用于预测受试者的预后，用于 选择对受试者的治疗，以及具有NAV3拷贝数变化的肿瘤的受试者的癌症治疗。 此外，本发明还涉及在受试者中使用选自特异性列表的至少一种基因和/或基因产物用于癌症治疗的拮抗剂，抗体或抑制性分子。 然而，本发明涉及一种诊断试剂盒，其包括用于检测生物样品中的NAV3拷贝数变化的工具和用于检测生物样品中选自特异性列表的至少一种基因或基因产物的过表达的工具。 本发明还涉及本发明的诊断试剂盒用于证明肿瘤或细胞亚群的恶性特征，用于预测具有结肠直肠肿瘤，脑肿瘤或表皮角化细胞肿瘤的受试者的预后，并且用于选择 治疗患有结肠直肠肿瘤，脑肿瘤或表皮角化细胞肿瘤的受试者。

    DIAGNOSTICS OF B-CELL LYMPHOMA
    6.
    发明申请
    DIAGNOSTICS OF B-CELL LYMPHOMA 审中-公开
    B细胞淋巴瘤的诊断

    公开(公告)号：US20100203536A1

    公开(公告)日：2010-08-12

    申请号：US12677558

    申请日：2008-09-22

    Applicant: Kai Krohn

    Inventor： Kai Krohn

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112

    Abstract: The present invention relates to the fields of genetics and oncology and provides methods and means for diagnosing and monitoring of patients having B-cell lymphomas, such methods and means allowing an early diagnosis of the B-cell lymphoma. Specifically, the present invention relates to a novel method and a biomarker for diagnosing B-cell lymphomas and for differentiating the B-cell lymphomas into prognostic groups of indolent and aggressive B-cell lymphomas.

    Abstract translation: 本发明涉及遗传学和肿瘤学领域，并提供了用于诊断和监测具有B细胞淋巴瘤的患者的方法和手段，允许B细胞淋巴瘤的早期诊断的方法和手段。 具体地，本发明涉及用于诊断B细胞淋巴瘤和将B细胞淋巴瘤分化为无痛和侵袭性B细胞淋巴瘤的预后组的新方法和生物标志物。

    METHODS AND USES INVOLVING GENETIC ABNORMALITIES AT CHROMOSOME 12
    7.
    发明申请
    METHODS AND USES INVOLVING GENETIC ABNORMALITIES AT CHROMOSOME 12 审中-公开
    涉及染色体遗传异常的方法和用途12

    公开(公告)号：US20100143907A1

    公开(公告)日：2010-06-10

    申请号：US12514638

    申请日：2006-11-13

    Applicant: Kai Krohn ,  Wael Hassan ,  Päivi Peltomäki ,  Markku Helle ,  Sonja Hahtola ,  Annamari Ranki

    Inventor： Kai Krohn ,  Wael Hassan ,  Päivi Peltomäki ,  Markku Helle ,  Sonja Hahtola ,  Annamari Ranki

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q1/6841 ,  C12Q2600/156

    Abstract: The present invention relates to the fields of genetics and oncology and provides methods for predicting and identifying tumors of epithelial origin. Specifically, the present invention relates to a novel method of predicting tumor initiation, tumor progression and/or carcinomas, the method comprising detecting genetic abnormality associated with tumors of epithelial origin. The present invention further relates to a novel method of identifying an individual with potential for developing carcinoma, the method comprising detection of genetic abnormalities. The present invention also relates to a method of predicting the progression of carcinomas and the transformation thereof to an aggressive variant, the method comprising detection of genetic abnormalities, which indicate the probability to develop carcinoma. The present invention also relates to a use of specific chromosomal region, a gene or a fragment thereof, and/or genetic markers for predicting tumor initiation, tumor progression and/or carcinoma. The present invention also relates to a use of specific chromosomal region or a gene or a fragment thereof in therapy, for the development of therapy, and for the preparation of a medicament for treating tumors of epithelial origin.

    Abstract translation: 本发明涉及遗传学和肿瘤学领域，并提供预测和鉴定上皮起源肿瘤的方法。 具体地，本发明涉及一种预测肿瘤起始，肿瘤进展和/或癌的新方法，该方法包括检测与上皮起源的肿瘤相关的遗传异常。 本发明还涉及鉴定具有发展癌潜力的个体的新方法，所述方法包括检测遗传异常。 本发明还涉及预测癌的进展及其转化为侵略性变体的方法，所述方法包括检测遗传异常，其指示发生癌的可能性。 本发明还涉及特定染色体区域，其基因或其片段的用途和/或用于预测肿瘤起始，肿瘤进展和/或癌症的遗传标记。 本发明还涉及特异性染色体区域或其基因或片段在治疗中的用途，用于治疗的发展，以及用于制备治疗上皮肿瘤的药物。

    Novel expression vectors and uses thereof
    10.
    发明申请
    Novel expression vectors and uses thereof 有权
    新型表达载体及其用途

    公开(公告)号：US20050026137A1

    公开(公告)日：2005-02-03

    申请号：US10476615

    申请日：2002-05-03

    Applicant: Kai Krohn ,  Vesna Blazevic ,  Marja Tahtinen ,  Mart Ustav ,  Urve Toots ,  Andres Mannjk ,  Annamari Ranki ,  Ene Ustav

    Inventor： Kai Krohn ,  Vesna Blazevic ,  Marja Tahtinen ,  Mart Ustav ,  Urve Toots ,  Andres Mannjk ,  Annamari Ranki ,  Ene Ustav

    IPC: A61K48/00 ,  C12N15/85 ,  C12Q1/70 ,  C12N15/86

    CPC classification number: C07K14/005 ,  A61K48/00 ,  A61K2039/53 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/203

    Abstract: The present invention relates to novel vectors, to DNA vaccines and gene therapeutics containing said vectors, to methods for the preparation of the vectors and DNA vaccines and gene therapeutics containing the vectors, and to therapeutic uses of said vectors. More specifically, the present invention relates to novel vectors comprising (a) an expression cassette of a gene of a nuclear-anchoring protein, which contains (i) a DNA binding domain capable of binding to a specific DNA sequence and (ii) a functional domain capable of binding to a nuclear component and (b) a multimerized DNA sequence forming a binding site for the anchoring protein, and optionally (c) one or more expression cassettes of a DNA sequence of interest. In particular the invention relates to vectors that lack a papilloma virus origin of replication. The nuclear-anchoring protein might be the E2 protein of Bovine Papilloma Virus type 1 or Epstein-Barr Virus Nuclear Antigen 1. The invention also relates to vectors that lack an origin of replication functional in a mammalian cell. The invention further relates to methods for expressing a DNA sequence of interest in a subject.

    Abstract translation: 本发明涉及新载体，DNA疫苗和含有所述载体的基因治疗剂，用于制备载体和DNA疫苗的方法以及含有载体的基因治疗剂以及所述载体的治疗用途。 更具体地说，本发明涉及包含（a）核锚定蛋白基因的表达盒的新载体，其包含（i）能够结合特定DNA序列的DNA结合结构域和（ii）功能性 能够结合核组分的结构域和（b）形成锚定蛋白的结合位点的多聚化DNA序列，以及任选（c）感兴趣的DNA序列的一个或多个表达盒。 特别地，本发明涉及缺乏乳头瘤病毒复制起点的载体。 核锚定蛋白可能是牛乳头瘤病毒1型或爱泼斯坦 - 巴尔病毒核抗原1的E2蛋白。本发明还涉及在哺乳动物细胞中缺乏复制起点的载体。 本发明还涉及在受试者中表达感兴趣的DNA序列的方法。

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