Rapid mercury-free photochemical microencapsulation/nanoencapsulation at ambient conditions

    公开(公告)号:US11666940B2

    公开(公告)日:2023-06-06

    申请号:US17714874

    申请日:2022-04-06

    摘要: A method of mercury-free photochemical micro-/nano-encapsulation of an active material is a process for obtaining Micro-/nano-capsules by means of curing by UV LED radiation at ambient or even cold temperatures. A stirrer photo-reactor made from glass or transparent plastics can be used but mixed flow reactor could be also employed. Appropriate mixing is sufficient to expose all droplets, which contain an active material surrounded by curable-shell materials in the emulsion to the LED radiation. Using the optimum light intensities and reactions' times is critical for encapsulating the active material with a high efficiency and producing a high quality micro-/nano-capsules, Solar monochromator device can also be used as long as it generate the same radiation with a narrow/single wavelengths as the LED device. Light emitted diode (LED) is a mercury-free UV radiation source with a long operating life time and an instant ON-Off, it has a high efficiency, a very low cooling requirements and cost-efficient in photochemical encapsulation. It reduces the time of microencapsulation from 6 hours to a less than 5 minutes. It has a significant decrease in manufacturing cost, waste-water, unconverted monomers, and leftover active phase change material (PCM) compared to other methods. Conversion of more than 90% of monomers can be achieved, and encapsulation efficiency can reach 100% at optimum conditions. This is in addition to the ability of this invented technology for encapsulate volatile and heat sensitive active materials at ambient as well as low temperatures. Normal glass or transparent plastics can be used as a reactor material. Only the matched useful wavelength radiation is emitted by LED without having other wavelengths which might have a bad impact on the encapsulation process.

    MULTIPLEX IMMUNOFLUORESCENCE DETECTION OF TARGET ANTIGENS

    公开(公告)号:US20230095395A1

    公开(公告)日:2023-03-30

    申请号:US17801386

    申请日:2021-02-25

    IPC分类号: G01N33/574

    摘要: A method of multispectral immunofluorescence imaging of a biological sample is described. The described method allows direct detection of seven or more target antigens simultaneously using directly labeled antibody fluorophore conjugates. The described method enables multiplex detection and analysis of a plurality of biomarkers simultaneously across an entire planar biological sample, providing unique spatio-temporal insights in immune-therapeutics and immuno-diagnostics.