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    Interleukin-3 (IL-3) multiple mutation polypeptides
    1.
    发明授权
    Interleukin-3 (IL-3) multiple mutation polypeptides 失效
    白细胞介素-3（IL-3）多重突变多肽

    公开(公告)号：US06458931B1

    公开(公告)日：2002-10-01

    申请号：US08469419

    申请日：1995-06-06

    申请人： S. Christopher Bauer ,  Mark Allen Abrams ,  Sarah Ruth Bradford-Goldberg ,  Maire Helena Caparon ,  Alan Michael Easton ,  Barbara Kure Klein ,  John Patrick McKearn ,  Peter Olins ,  Kumnan Paik ,  John Warren Thomas

    发明人： S. Christopher Bauer ,  Mark Allen Abrams ,  Sarah Ruth Bradford-Goldberg ,  Maire Helena Caparon ,  Alan Michael Easton ,  Barbara Kure Klein ,  John Patrick McKearn ,  Peter Olins ,  Kumnan Paik ,  John Warren Thomas

    IPC分类号： C07K100

    CPC分类号： C07K14/5403 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/02 ,  Y10S930/141

    摘要： The present invention relates to recombinant human interleukin-3 (hIL-3) variant or mutant proteins (muteins). These hIL-3 muteins contain amino acid substitutions and may also have amino acid deletions at both the N- and C-termini. The invention also relates to pharmaceutical compositions containing the hIL-3 muteins and methods for using them. Additionally, the present invention relates to recombinant expression vectors comprising nucleotide sequences encoding the hIL-3 muteins, related microbial expression systems, and processes for making the hIL-3 muteins using the microbial expression systems. Included in the present invention are deletion mutants of hIL-3 in which from 1 to 14 amino acids have been deleted from the N-terminus, and from 1 to 15 amino acids (a.a.119 to 133) have been deleted from the C-terminus, and which also contain amino acid substitutions in the polypeptide. These hIL-3 multiple mutation polypeptides may have biological activities similar to or better than hIL-3 and, in some cases, may also have an improved side effect profile.

    摘要翻译： 本发明涉及重组人白细胞介素-3（hIL-3）变体或突变蛋白（突变蛋白）。 这些hIL-3突变蛋白含有氨基酸取代，并且在N-和C-末端也可具有氨基酸缺失。 本发明还涉及含有hIL-3突变蛋白的药物组合物及其使用方法。 另外，本发明涉及包含编码hIL-3突变蛋白的核苷酸序列，相关微生物表达系统和使用微生物表达系统制备hIL-3突变蛋白的方法的重组表达载体。 本发明包括hIL-3的缺失突变体，其中从N末端缺失1至14个氨基酸，从C末端缺失1至15个氨基酸（aa119至133） ，并且其还在多肽中含有氨基酸取代。 这些hIL-3多重突变多肽可具有类似于或优于hIL-3的生物学活性，并且在一些情况下也可具有改善的副作用特征。