Mammalian telophase oocyte enucleation
    5.
    发明授权
    Mammalian telophase oocyte enucleation 有权
    哺乳动物端粒酶卵母细胞摘除

    公开(公告)号:US07592503B2

    公开(公告)日:2009-09-22

    申请号:US10913013

    申请日:2004-08-06

    IPC分类号: C12N15/00

    摘要: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.

    摘要翻译: 本发明涉及克隆动物的方法。 特别地,本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法,从而获得核转移胚胎,并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。

    Methods for cloning animals
    9.
    发明申请
    Methods for cloning animals 有权
    克隆动物的方法

    公开(公告)号:US20050144663A1

    公开(公告)日:2005-06-30

    申请号:US10913013

    申请日:2004-08-06

    摘要: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.

    摘要翻译: 本发明涉及克隆动物的方法。 特别地,本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法,从而获得核转移胚胎,并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。

    Methods for cloning mammals using telophase oocytes
    10.
    发明授权
    Methods for cloning mammals using telophase oocytes 有权
    使用端粒酶卵母细胞克隆哺乳动物的方法

    公开(公告)号:US06781030B1

    公开(公告)日:2004-08-24

    申请号:US09432906

    申请日:1999-11-02

    IPC分类号: C12N1500

    摘要: The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.

    摘要翻译: 本发明涉及克隆动物的方法。 特别地,本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法,从而获得核转移胚胎,并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。