公开(公告)号：US10117967B2

公开(公告)日：2018-11-06

申请号：US15729668

申请日：2017-10-11

申请人： Maryam Eslami ,  Parvaneh Reshteh Ahmadi ,  Alireza Eslami

发明人： Maryam Eslami ,  Parvaneh Reshteh Ahmadi ,  Alireza Eslami

IPC分类号： A61L27/20 ,  A61L27/18 ,  A61L27/38 ,  A61L27/22 ,  C08L67/02 ,  C07K14/78 ,  C12N5/077 ,  C08B37/08 ,  C08J3/075

摘要： The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.

公开(公告)号：US20180117215A1

公开(公告)日：2018-05-03

申请号：US15729668

申请日：2017-10-11

申请人： Maryam Eslami ,  PARVANEH RESHTEH AHMADI ,  Alireza Eslami

发明人： Maryam Eslami ,  PARVANEH RESHTEH AHMADI ,  Alireza Eslami

IPC分类号： A61L27/18 ,  A61L27/38 ,  A61L27/22 ,  A61L27/20 ,  C08B37/08 ,  C08L67/02 ,  C07K14/78 ,  C12N5/077

CPC分类号： A61L27/18 ,  A61L27/20 ,  A61L27/222 ,  A61L27/3804 ,  A61L27/52 ,  A61L27/60 ,  C07K14/78 ,  C08B37/0072 ,  C08J3/075 ,  C08L67/025 ,  C08L2203/02 ,  C12N5/0656 ,  C08L5/08 ,  C08L67/04

摘要： The embodiments herein disclose a method of fabricating composite scaffolds for skin tissue regeneration. The methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) are synthesized. The poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are synthesized. The hydrogel is synthesized. The composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds is fabricated. A plurality of physico-chemical characteristics of the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds are analysed. The physico-chemical characteristics comprises mechanical properties, swelling ratio and enzymatic degradation and scanning electron microscope imaging. The fibroblast cells are encapsulated within the composite scaffold comprising hydrogel and poly (glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) microfibrous scaffolds and hydrogels. The fibroblast cells are seeded on composite scaffold and PGS-PCL scaffold. The fibroblast cell viability, fibroblast cell attachment, fibroblast cell spreading, fibroblast cell proliferation and fibroblast cell metabolism are analysed in composite scaffolds, PGS-PCL scaffolds and hydrogels.