公开(公告)号：US4729950A

公开(公告)日：1988-03-08

申请号：US760036

申请日：1985-07-29

申请人： Larry J. Kricka ,  Angela M. O'Toole ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

发明人： Larry J. Kricka ,  Angela M. O'Toole ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

IPC分类号： G01N33/52 ,  C12Q1/28 ,  G01N21/76 ,  G01N33/532 ,  G01N33/535

CPC分类号： G01N33/535 ,  C12Q1/28 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/80

摘要： It has been a problem that the chemiluminescent emission produced by the peroxidase-catalyzed oxidation of a 2,3-dihydro-1,4-phthalazinedione is often weak or gives a poor signal-background ratio and therefore does not give the high degree of sensitivity required for assay purposes, particularly for assay of peroxidase used to label a reagent for example in ELISA. The present invention solves the problem by the finding the certain narrowly defined aromatic amines are enhancers of the reaction and accordingly provides for their use in an assay and kit. The amines are of general formula (I) ##STR1## wherein the R symbols (that is, R, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6) have any of the meanings (a) to (j) given below, all other R symbols in each meaning are hydrogen atoms and the fused rings are to be read in the same configurational sense as formula I:(a) R=R.sup.1 =CH.sub.3 ; R.sup.4 = ##STR2## (b) R.sup.4 = ##STR3## cyclohexyl, or alkyl or alkoxy having 1 to 4 carbon atoms (c) R.sup.2, R.sup.3 and R.sup.4 together represent the polycyclic fused ring system: ##STR4## (d) R.sup.3 and R.sup.4 together represent the fused ring or ring system ##STR5## (e) R.sup.3 and R.sup.4 together represent the fused ring system ##STR6## and R.sup.5 and R.sup.6 together represent the fused ring ##STR7## (f) R.sup.2 =NH.sub.2 and R.sup.4 = ##STR8## (g) R.sup.2 =R.sup.6 =CH.sub.3 and R.sup.4 = ##STR9## (h) R.sup.3 =C.sub.2 H.sub.5 (i) R.sup.2 =R.sup.4 =CH.sub.3 and(j) R.sup.3 =R.sup.4 =CH.sub.3.

摘要翻译： 由2,3-二氢-1,4-酞嗪二酮的过氧化物酶催化氧化产生的化学发光辐射常常较弱或信号背景差较弱，因此不会产生高灵敏度的问题 用于测定目的，特别是用于测定用于标记例如ELISA中的试剂的过氧化物酶。 本发明通过发现某些狭义定义的芳族胺是反应的增强剂来解决该问题，因此提供了它们在测定和试剂盒中的应用。 胺具有通式（I）其中R符号（即R，R 1，R 2，R 3，R 4，R 5和R 6）具有给定的（a）至（j） 在下文中，每个含义中的所有其他R符号是氢原子，并且稠合环将以与式I相同的构型意义读取：（a）R = R 1 = CH 3; （c）R2 = R3，R4和R4分别代表多环稠环体系：（R）R 4 = （e）R3和R4一起代表稠合环系统，而R 5和R 6一起代表稠环，（f）R2 = NH2，R4 = （g）R2 = R6 = CH3，R4 =（h）R3 = C2H5（i）R2 = R4 = CH3，（j）R3 = R4 = CH3。