公开(公告)号：US07494770B2

公开(公告)日：2009-02-24

申请号：US10426674

申请日：2003-04-30

申请人： Peter Wilding ,  Larry J. Kricka

发明人： Peter Wilding ,  Larry J. Kricka

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/483 ,  G01N33/555

CPC分类号： C12Q1/686 ,  B01D61/18 ,  B01D67/003 ,  B01D71/02 ,  B01D2325/028 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1861 ,  B01L2400/0487 ,  C40B40/06 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/809 ,  Y10T436/25375

摘要： Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber). The PCR chamber is provided with the sample polynucleotide, polymerase, nucleoside triphosphates, primers and other reagents required for the polymerase chain reaction, and the device is provided with a device for thermally controlling the temperature of the contents of the reaction chamber at a temperature controlled to dehybridize double stranded polynucleotide, to anneal the primers, and to polymerize and amplify the polynucleotide.

摘要翻译： 公开了通过进行多核苷酸聚合反应来扩增样品中预选择的多核苷酸的装置。 这些装置包括微结构的基板，以限定从进口端口延伸的样品入口端口和中尺度流动系统。 中尺度流动系统包括与入口端口流体连通的多核苷酸聚合反应室，其具有聚合和扩增预选择的多核苷酸所需的试剂。 在一个实施方案中，该装置可用于在反应室（PCR室）中实施聚合酶链式反应（PCR）。 PCR室设置有聚合酶链反应所需的样品多核苷酸，聚合酶，核苷三磷酸，引物和其他试剂，并且该装置设置有用于在受温度控制的温度控制反应室的内容物的温度的装置 将双链多核苷酸脱杂交，使引物退火，并聚合和扩增多核苷酸。

公开(公告)号：US06953676B1

公开(公告)日：2005-10-11

申请号：US09212029

申请日：1998-12-15

申请人： Peter Wilding ,  Larry J. Kricka

发明人： Peter Wilding ,  Larry J. Kricka

IPC分类号： B01D61/18 ,  B01D67/00 ,  B01D71/02 ,  B01J19/00 ,  B01L7/00 ,  C12M1/26 ,  C12M1/34 ,  C12M3/08 ,  C12Q1/68 ,  C40B40/06 ,  C12P19/34 ,  C12M1/00 ,  G01N1/00 ,  G01N15/00

CPC分类号： C12Q1/686 ,  B01D61/18 ,  B01D67/003 ,  B01D71/02 ,  B01D2325/028 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/1822 ,  B01L2300/185 ,  B01L2300/1861 ,  B01L2400/0487 ,  C40B40/06 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/809 ,  Y10T436/25375

摘要： Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber). The PCR chamber is provided with the sample polynucleotide, polymerase, nucleoside triphosphates, primers and other reagents required for the polymerase chain reaction, and the device is provided with means for thermally controlling the temperature of the contents of the reaction chamber at a temperature controlled to dehybridize double stranded polynucleotide, to anneal the primers, and to polymerize and amplify the polynucleotide.

摘要翻译： 公开了通过进行多核苷酸聚合反应来扩增样品中预选择的多核苷酸的装置。 这些装置包括微结构的基板，以限定从进口端口延伸的样品入口端口和中尺度流动系统。 中尺度流动系统包括与入口端口流体连通的多核苷酸聚合反应室，其具有聚合和扩增预选多核苷酸所需的试剂。 在一个实施方案中，该装置可用于在反应室（PCR室）中实施聚合酶链式反应（PCR）。 PCR室设置有聚合酶链式反应所需的样品多核苷酸，聚合酶，核苷三磷酸酯，引物和其他试剂，并且该装置具有用于热控制反应室内的温度的装置，该温度控制到 脱杂交双链多核苷酸，以退火引物，并聚合和扩增多核苷酸。

公开(公告)号：US06184029B2

公开(公告)日：2001-02-06

申请号：US09237532

申请日：1999-01-27

申请人： Peter Wilding ,  Larry J. Kricka

发明人： Peter Wilding ,  Larry J. Kricka

IPC分类号： C12M134

CPC分类号： C12Q1/686 ,  B01D61/18 ,  B01D67/003 ,  B01D67/0034 ,  B01D67/0058 ,  B01D67/0062 ,  B01D71/02 ,  B01D2325/028 ,  B01F5/0601 ,  B01F15/0264 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01J2219/00828 ,  B01J2219/00873 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/086 ,  C12M41/46 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/81 ,  Y10S436/807 ,  C12Q2565/629

摘要： A mesoscale sample preparation device capable of providing microvolume test samples, separated into a cell-enriched fraction and a fraction of reduced cell content, for performing various analyses, such as binding assays, determinations involving polynucleotide amplification and the like. Analytical systems including such devices are also disclosed.

摘要翻译： 一种能够提供微量体积测试样品的中尺度样品制备装置，其分离成富含细胞的级分和减少的细胞含量的部分，用于进行各种分析，例如结合测定，涉及多核苷酸扩增的测定等。 还公开了包括这种装置的分析系统。

公开(公告)号：US5955029A

公开(公告)日：1999-09-21

申请号：US614242

申请日：1996-03-12

申请人： Peter Wilding ,  Larry J. Kricka

发明人： Peter Wilding ,  Larry J. Kricka

IPC分类号： B01D61/18 ,  B01D67/00 ,  B01D71/02 ,  B01F5/06 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12M1/26 ,  C12M1/34 ,  C12M3/00 ,  C12M3/08 ,  C12Q1/68 ,  C40B40/06 ,  G01N15/06 ,  C12P19/34

CPC分类号： C12Q1/686 ,  B01D61/18 ,  B01D67/0058 ,  B01D67/0072 ,  B01D71/02 ,  B01J19/0046 ,  B01J19/0093 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01F5/0601 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L3/502707 ,  B01L3/502753 ,  B01L9/527 ,  C12M41/46 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/904

摘要： Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber). The PCR chamber is provided with the sample polynucleotide, polymerase, nucleoside triphosphates, primers and other reagents required for the polymerase chain reaction, and the device is provided with means for thermally controlling the temperature of the contents of the reaction chamber at a temperature controlled to dehybridize double stranded polynucleotide, to anneal the primers, and to polymerize and amplify the polynucleotide.

摘要翻译： 公开了通过进行多核苷酸聚合反应来扩增样品中的预选多核苷酸的装置。 这些装置包括微结构的基板，以限定从进口端口延伸的样品入口端口和中尺度流动系统。 中尺度流动系统包括与入口端口流体连通的多核苷酸聚合反应室，其具有聚合和扩增预选择的多核苷酸所需的试剂。 在一个实施方案中，该装置可用于在反应室（PCR室）中实施聚合酶链式反应（PCR）。 PCR室设置有聚合酶链式反应所需的样品多核苷酸，聚合酶，核苷三磷酸酯，引物和其他试剂，并且该装置具有用于热控制反应室内的温度的装置，该温度控制到 脱杂交双链多核苷酸，以退火引物，并聚合和扩增多核苷酸。

公开(公告)号：US5512451A

公开(公告)日：1996-04-30

申请号：US217845

申请日：1994-03-25

申请人： Larry J. Kricka

发明人： Larry J. Kricka

IPC分类号： C12Q1/28 ,  C07H15/00

CPC分类号： C12Q1/28 ,  Y10S435/968

摘要： In enhanced chemiluminescent (ECL) reactions of a fused aromatic diacyl cyclic hydrazide such as luminol, a peroxidase enzyme catalyst, an oxidant such as hydrogen peroxide and an enhancer, it has been found advantageous to use a combination of an organoboron enhancer such as 4-biphenylboronic acid with a non boron-containing enhancer, especially a phenolic or aromatic amine enhancer, particularly 4-iodophenol. ECL reactions are useful in diagnostic assay.

摘要翻译： 在稠合芳族二酰基环状酰肼如鲁米诺，过氧化物酶催化剂，氧化剂如过氧化氢和增强剂的增强化学发光（ECL）反应中，已经发现有利的是使用有机硼增强剂如4- 联苯硼酸与非硼增强剂，特别是酚或芳族胺增强剂，特别是4-碘苯酚。 ECL反应可用于诊断测定。

公开(公告)号：US5302533A

公开(公告)日：1994-04-12

申请号：US866043

申请日：1992-04-10

申请人： Larry J. Kricka

发明人： Larry J. Kricka

IPC分类号： G01N33/577 ,  G01N33/58 ,  G01N33/542 ,  C07K15/06 ,  G01N33/563

CPC分类号： G01N33/582 ,  G01N33/577 ,  Y10S435/968

摘要： A method of increasing the light output from a chemiluminescent reaction of a dihydrophthalazinedione (DPD), and an oxidant, which comprises carrying out said reaction in the presence of at least one antibody, raised against an intermediate species of said chemiluminescent reaction.

摘要翻译： 增加二氢酞嗪二酮（DPD）和氧化剂的化学发光反应的光输出的方法，其包括在至少一种抗体的存在下进行所述反应而产生的所述化学发光反应的中间物种。

公开(公告)号：US4879097A

公开(公告)日：1989-11-07

申请号：US848134

申请日：1986-04-04

申请人： Thomas P. Whitehead ,  Gary H. G. H. Thorpe ,  Larry J. Kricka ,  John E. C. Gibbons ,  Roger A. Bunce

发明人： Thomas P. Whitehead ,  Gary H. G. H. Thorpe ,  Larry J. Kricka ,  John E. C. Gibbons ,  Roger A. Bunce

IPC分类号： G01N35/00 ,  B01L3/02 ,  G01N21/76

CPC分类号： G01N21/76 ,  B01L3/021 ,  G01N2201/04

摘要： A recording apparatus has a holder for a plurality of reaction vessels including a support plate (11) having an array of holes (15) therein for receiving an array of reaction vessels (16), a housing (10) for receiving the holder and being sealed to prevent entry of stray light, an openable cover (24, 249) for the housing, a film back (12) for holding a photographic film adjacent the underside of the plate (11), and a removable shutter (13) for interposition between the film and the support plate.

公开(公告)号：US07919019B2

公开(公告)日：2011-04-05

申请号：US11912589

申请日：2006-04-26

申请人： Jason Y Park ,  Larry J Kricka

发明人： Jason Y Park ,  Larry J Kricka

IPC分类号： F21K2/06

CPC分类号： F21K2/06

摘要： The present invention relates to nanostructures for use in luminescent devices.

摘要翻译： 本发明涉及用于发光装置的纳米结构。

公开(公告)号：US07771985B2

公开(公告)日：2010-08-10

申请号：US11696365

申请日：2007-04-04

申请人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

发明人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

IPC分类号： C12M3/00

CPC分类号： B01L3/5027 ,  B01F5/0601 ,  B01F15/0264 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/686 ,  C12Q2565/629 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S977/923 ,  Y10S977/953

摘要： Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.

摘要翻译： 公开了用于检测流体样品中预选分析物的存在的装置。 这些装置包括微结构以限定样品入口的基底和包括从入口延伸的样品流动通道的中尺度流动系统。 中尺度流动系统还包括与包含用于特异性结合分析物的结合部分的流动通道流体连通的分析物检测区域。 检测区域的中尺度尺寸足够小以增强结合部分和分析物的结合。 结合部分可以被固定在检测区域中。 本发明的中尺度检测系统可用于广泛的应用，包括细胞或大分子的检测，或用于监测反应或细胞培养生长。

公开(公告)号：US07018830B2

公开(公告)日：2006-03-28

申请号：US10348438

申请日：2003-01-21

申请人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

发明人： Peter Wilding ,  Larry J. Kricka ,  Jay N. Zemel

IPC分类号： C12M3/00

CPC分类号： B01L3/5027 ,  B01F5/0601 ,  B01F15/0264 ,  B01J19/0093 ,  B01J2219/0059 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/146 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/1827 ,  B01L2300/1861 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/686 ,  C12Q2565/629 ,  C40B40/06 ,  Y10S366/03 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S977/923 ,  Y10S977/953

摘要： Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.