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    Apparatus and method for luminescent determination of concentration of an analyte in a sample
    3.
    发明授权
    Apparatus and method for luminescent determination of concentration of an analyte in a sample 失效
    用于发光测定样品中分析物浓度的装置和方法

    公开(公告)号：US4366118A

    公开(公告)日：1982-12-28

    申请号：US189930

    申请日：1980-02-14

    申请人： Roger A. Bunce ,  Timothy J. N. Carter ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

    发明人： Roger A. Bunce ,  Timothy J. N. Carter ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

    IPC分类号： G01N33/58 ,  G01N21/01 ,  G01N21/76

    CPC分类号： G01N21/76

    摘要： The apparatus comprises a movable member (10) which carries a plurality of cuvettes (11) containing material under test. Control means cause the member (10) to be moved to bring the cuvettes (11) successively into register with a photomultiplier (16), and in that position reagent is automatically added to a cuvette, giving rise to a luminescent reaction. Light from the reaction is detected by the photo-multiplier (16) and the resultant signal processed and recorded. The control means is triggered to stop the member (10), add reagent, record the signal from the luminescent reaction, and restart the member, from a photocell (28) which is illuminated through the member (10) only when a cuvette (11) is in register with the photomultiplier (16).According to the method, sample material containing the analyte for determination is mixed with a known amount or concentration of the same analyte labelled with a substance which is capable of taking part in a luminescent reaction. The mixture is then reacted with a known amount of another substance capable of reacting with the analyte and giving rise to a luminescent reaction with the labelling substance. From quantitative observation of the light from the luminescent reaction the amount or concentration of the analyte in the sample may be deduced.

    摘要翻译： PCT No.PCT / GB79 / 00102 Sec。 371日期1980年2月14日 102（e）1980年2月14日PCT PCT。 出版物WO80 / 00100 PCT 日期为1980年1月24日。该装置包括可移动构件（10），其携带包含被测材料的多个比色皿（11）。 控制装置使得构件（10）移动以使比色杯（11）依次与光电倍增器（16）对准，并且在该位置，试剂自动添加到比色杯中，引起发光反应。 通过光电倍增器（16）检测反应的光，并对所得信号进行处理和记录。 控制装置被触发以停止构件（10），添加试剂，记录来自发光反应的信号，并从仅通过构件（10）照射的光电池（28）重新启动构件，只有当比色杯（11 ）与光电倍增管（16）对齐。 根据该方法，将含有用于测定的分析物的样品材料与能够参与发光反应的物质标记的相同分析物的已知量或浓度混合。 然后将混合物与已知量的能够与分析物反应并产生与标记物质的发光反应的另一种物质反应。 通过定量观察发光反应的光，可以推导出样品中分析物的量或浓度。

    Enhanced luminescent and luminometric assay
    5.
    发明授权
    Enhanced luminescent and luminometric assay 失效
    增强发光和光度测定

    公开(公告)号：US4842997A

    公开(公告)日：1989-06-27

    申请号：US551985

    申请日：1983-10-27

    申请人： Timothy J. N. Carter ,  Carol J. Groucutt ,  Richard A. W. Stott ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

    发明人： Timothy J. N. Carter ,  Carol J. Groucutt ,  Richard A. W. Stott ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

    IPC分类号： G01N21/76 ,  C12Q1/28 ,  G01N33/58

    CPC分类号： C12Q1/28 ,  G01N33/581 ,  Y10S435/81 ,  Y10S435/968

    摘要： Assays, e.g. immunoassays, based on the chemiluminescent reaction between a peroxidase enzyme, e.g. horse radish peroxidase, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione, e.g. luminol or isoluminol and an oxidant, e.g. hydrogen peroxide or a perborate, are improved by carrying out the reaction in the presence of a 6-hydroxybenzothiazole, especially 6-hydroxybenzothiazole itself or firefly luciferin. Light emission from the reaction is significantly enhanced by the 6-hydroxybenzothiazole.

    摘要翻译： PCT No.PCT / GB83 / 00058 Sec。 371日期1983年10月27日第 102（e）日期1983年10月27日PCT提交1983年2月25日PCT公布。 公开号WO83 / 03104 日期：1983年9月15日。 基于过氧化物酶之间的化学发光反应的免疫测定。 辣根过氧化物酶，化学发光2,3-二氢-1,4-酞嗪二酮，例如 鲁米诺或异鲁米诺和氧化剂，例如 过氧化氢或过硼酸盐，通过在6-羟基苯并噻唑，特别是6-羟基苯并噻唑本身或萤火虫荧光素的存在下进行反应得到改善。 由6-羟基苯并噻唑显着增强反应的光发射。

    Enhanced luminescent or luminometric assay
    8.
    发明授权
    Enhanced luminescent or luminometric assay 失效
    增强发光或发光测定

    公开(公告)号：US4729950A

    公开(公告)日：1988-03-08

    申请号：US760036

    申请日：1985-07-29

    申请人： Larry J. Kricka ,  Angela M. O'Toole ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

    发明人： Larry J. Kricka ,  Angela M. O'Toole ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

    IPC分类号： G01N33/52 ,  C12Q1/28 ,  G01N21/76 ,  G01N33/532 ,  G01N33/535

    CPC分类号： G01N33/535 ,  C12Q1/28 ,  Y10S435/81 ,  Y10S435/968 ,  Y10S435/975 ,  Y10S436/80

    摘要： It has been a problem that the chemiluminescent emission produced by the peroxidase-catalyzed oxidation of a 2,3-dihydro-1,4-phthalazinedione is often weak or gives a poor signal-background ratio and therefore does not give the high degree of sensitivity required for assay purposes, particularly for assay of peroxidase used to label a reagent for example in ELISA. The present invention solves the problem by the finding the certain narrowly defined aromatic amines are enhancers of the reaction and accordingly provides for their use in an assay and kit. The amines are of general formula (I) ##STR1## wherein the R symbols (that is, R, R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6) have any of the meanings (a) to (j) given below, all other R symbols in each meaning are hydrogen atoms and the fused rings are to be read in the same configurational sense as formula I:(a) R=R.sup.1 =CH.sub.3 ; R.sup.4 = ##STR2## (b) R.sup.4 = ##STR3## cyclohexyl, or alkyl or alkoxy having 1 to 4 carbon atoms (c) R.sup.2, R.sup.3 and R.sup.4 together represent the polycyclic fused ring system: ##STR4## (d) R.sup.3 and R.sup.4 together represent the fused ring or ring system ##STR5## (e) R.sup.3 and R.sup.4 together represent the fused ring system ##STR6## and R.sup.5 and R.sup.6 together represent the fused ring ##STR7## (f) R.sup.2 =NH.sub.2 and R.sup.4 = ##STR8## (g) R.sup.2 =R.sup.6 =CH.sub.3 and R.sup.4 = ##STR9## (h) R.sup.3 =C.sub.2 H.sub.5 (i) R.sup.2 =R.sup.4 =CH.sub.3 and(j) R.sup.3 =R.sup.4 =CH.sub.3.

    摘要翻译： 由2,3-二氢-1,4-酞嗪二酮的过氧化物酶催化氧化产生的化学发光辐射常常较弱或信号背景差较弱，因此不会产生高灵敏度的问题 用于测定目的，特别是用于测定用于标记例如ELISA中的试剂的过氧化物酶。 本发明通过发现某些狭义定义的芳族胺是反应的增强剂来解决该问题，因此提供了它们在测定和试剂盒中的应用。 胺具有通式（I）其中R符号（即R，R 1，R 2，R 3，R 4，R 5和R 6）具有给定的（a）至（j） 在下文中，每个含义中的所有其他R符号是氢原子，并且稠合环将以与式I相同的构型意义读取：（a）R = R 1 = CH 3; （c）R2 = R3，R4和R4分别代表多环稠环体系：（R）R 4 = （e）R3和R4一起代表稠合环系统，而R 5和R 6一起代表稠环，（f）R2 = NH2，R4 = （g）R2 = R6 = CH3，R4 =（h）R3 = C2H5（i）R2 = R4 = CH3，（j）R3 = R4 = CH3。

    Dispensing device and recording apparatus
    9.
    发明授权
    Dispensing device and recording apparatus 失效
    点胶装置和记录装置

    公开(公告)号：US4593728A

    公开(公告)日：1986-06-10

    申请号：US626856

    申请日：1984-06-27

    申请人： Thomas P. Whitehead ,  Gary H. G. H. Thorpe ,  Larry J. Kricka ,  John E. C. Gibbons ,  Roger A. Bunce

    发明人： Thomas P. Whitehead ,  Gary H. G. H. Thorpe ,  Larry J. Kricka ,  John E. C. Gibbons ,  Roger A. Bunce

    IPC分类号： G01N35/00 ,  B01L3/02 ,  G01N21/76 ,  B65B3/04

    CPC分类号： G01N21/76 ,  B01L3/021 ,  G01N2201/04

    摘要： A dispensing device, for dispensing reagent into an array of reaction wells (16) in a photographic recording apparatus, comprises an array of tubes (28) which are mounted in a support (23). The support (23) contains a common chamber (29) into which the tops of all the tubes (28) open. The chamber (29) is connected with atmosphere via an opening (32) which is sealable manually to retain liquid in the tubes (28) when the latter are immersed in a reservoir (40) and which is unsealed to dispense the liquid. The recording apparatus has a housing (10) closable at the top by part of the support (23). A plate (11) having an array of holes (15) therethrough rests on a shutter (13) disposed above a photographic film holder (12). The plate (11) carries the reaction wells (16) in the holes (15).

    摘要翻译： PCT No.PCT / GB83 / 00287 Sec。 371日期1984年6月27日第 102（e）日期1984年6月27日PCT提交1983年11月14日PCT公布。 出版物WO84 / 02090 日期：1984年6月7日。一种用于将试剂分配到摄影记录装置中的反应井（16）阵列中的分配装置，包括安装在支撑件（23）中的管阵列（28）。 支撑件（23）包含公共室（29），所有管（28）的顶部打开。 室（29）经由可手动密封的开口（32）与大气连接，当开口（32）浸入储存器（40）中时，将液体保持在管（28）中，并且其未被密封以分配液体。 该记录装置具有一个壳体（10），可以在顶部由支撑件（23）的一部分封闭。 具有穿过孔阵列（15）的板（11）搁置在设置在照相胶片保持器（12）上方的挡板（13）上。 板（11）在孔（15）中承载反应孔（16）。

    Apparatus for testing a liquid sample
    10.
    发明授权
    Apparatus for testing a liquid sample 失效
    用于测试液体样品的装置

    公开(公告)号：US4356722A

    公开(公告)日：1982-11-02

    申请号：US203572

    申请日：1980-11-05

    申请人： Roger A. Bunce ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

    发明人： Roger A. Bunce ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

    IPC分类号： G01N35/00 ,  G01N35/02

    CPC分类号： G01N35/02 ,  G01N35/00

    摘要： Apparatus for testing at least one liquid sample, by immersion therein of a probe for coaction between said probe and sample, having a reloadable sample carrier for at least one sample container; a reloadable probe carrier for at least one probe; a frame having locating means for relatively locating the sample carrier and the probe carrier laterally in the frame, parallel motion means for guiding the probe longitudinally into the sample container and stop means for locating the probe at a predetermined distance into the sample container, locking means preventing the probe from being withdrawn more than a predetermined distance in the outward direction from the sample container, timing and control means for releasing said locking means after a predetermined time, and self acting withdrawal means for withdrawing the probe and probe carrier to a position clear of the sample container; the said sample carrier and probe carrier being arranged so as to be severally removable away from the said frame.

    摘要翻译： 用于测试至少一种液体样品的装置，通过浸入其中用于所述探针和样品之间的共同作用的探针，具有用于至少一个样品容器的可重新加载的样品载体; 用于至少一个探针的可重新加载的探针载体; 具有用于将样品载体和探针载体横向地相对定位在框架中的定位装置的框架，用于将探针纵向引导到样品容器中的平行运动装置和用于将探针定位在预定距离进入样品容器的停止装置，锁定装置 防止探针从样品容器向外的方向被抽出超过预定距离，在预定时间之后释放所述锁定装置的定时和控制装置，以及用于将探针和探针载体抽出到位置的自动提取装置 的样品容器; 所述样品载体和探针载体被布置成从所述框架分开移除。