-
公开(公告)号：US20110117605A1
公开(公告)日：2011-05-19
申请号：US12989340
申请日：2009-04-23
Applicant: Anne Bondgaard Tolstrup , Lars Soegaard Nielsen , Dietmar Weilguny , Christian Müller , Finn C. Wiberg , Jonas Heilskov Graversen
Inventor： Anne Bondgaard Tolstrup , Lars Soegaard Nielsen , Dietmar Weilguny , Christian Müller , Finn C. Wiberg , Jonas Heilskov Graversen
IPC: C12P21/00
CPC classification number: C07K16/00 , A61K2039/507
Abstract: The invention relates to methods for manufacturing drug products comprising at least two distinct members of a polyclonal protein, for example a polyclonal antibody, where each distinct member is expressed by a separate population of cells. The methods involve at least an initial step in which the cell populations expressing the distinct members of the polyclonal protein are cultured separately. The individual cell populations, or proteins expressed by the individual cell populations, are combined at a later point of the upstream or downstream processing to result in a single drug product comprising the distinct members of the polyclonal protein.
Abstract translation: 本发明涉及用于制备药物制品的方法，其包含多克隆蛋白质，例如多克隆抗体的至少两个不同成员，其中每个不同的成员由单独的细胞群体表达。 该方法涉及至少一个初始步骤，其中分别表达多克隆蛋白质的不同成员的细胞群体。 单个细胞群体或由各个细胞群体表达的蛋白质在上游或下游处理的稍后点组合以产生包含多克隆蛋白质的不同成员的单一药物产品。
-
Patent Agency Ranking
公开(公告)号：US07910332B2
公开(公告)日：2011-03-22
申请号：US12153809
申请日：2008-05-23
Applicant: Lars Soegaard Nielsen , Dietmar Weilguny , Anne Bondgaard Tolstrup , Finn C. Wiberg , Christian Muller
Inventor： Lars Soegaard Nielsen , Dietmar Weilguny , Anne Bondgaard Tolstrup , Finn C. Wiberg , Christian Muller
CPC classification number: C12N15/1086 , C07K16/00 , C07K16/081
Abstract: The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.
Abstract translation: 本发明涉及制备重组多克隆蛋白质组合物，特别是重组多克隆抗体组合物的方法。 该方法包括获得用变体核酸序列的集合转染的细胞的集合，其中集合中的每个细胞被转染并能够表达编码多克隆蛋白的不同成员的集合的一个成员。 细胞在合适的条件下培养，用于表达从细胞或培养物上清液获得的多克隆蛋白质。 通过用载体的集合转染将核酸序列引入细胞。 本发明方法适用于制备用于治疗用途的重组多克隆抗体。
-
3.发明申请公开(公告)号：US20120322690A1
公开(公告)日：2012-12-20
申请号：US13493623
申请日：2012-06-11
Applicant: Søren Kofoed RASMUSSEN , Anne Bondgaard Tolstrup , Søren Bregenholt Frederiksen , John Haurum
Inventor： Søren Kofoed RASMUSSEN , Anne Bondgaard Tolstrup , Søren Bregenholt Frederiksen , John Haurum
CPC classification number: C07K16/34 , C07K16/005 , C07K2317/21 , C07K2317/55 , C07K2317/77
Abstract: The invention relates to a method for manufacturing an anti-RhD recombinant polyclonal antibody composition (anti-RhD rpAb). The method comprises obtaining a collection of cells transfected with a library of anti-RhD antibody expression vectors, wherein each cell in the collection is capable of expressing from a VH and VL comprising nucleic acid segment, one member of the library, which encodes a distinct member of anti-RhD recombinant polyclonal antibody composition and is located at the same site in the genome of individual cells in said collection. The cells are cultured under suitable conditions for expression of the recombinant polyclonal antibody, which is obtained from the cells or culture supernatant. Nucleic acid segments encoding the anti-RhD rpAb are introduced into the cells by transfection with a library of vectors for site-specific integration. The method is suitable for manufacturing anti-RhD rpAb, thereby making available a superior replacement of plasma-derived prophylactic and therapeutic immunoglobulin products.
Abstract translation: 本发明涉及一种制备抗RhD重组多克隆抗体组合物（抗RhD rpAb）的方法。 该方法包括获得用抗RhD抗体表达载体文库转染的细胞的集合，其中集合中的每个细胞能够从包含核酸片段的VH和VL表达，文库的一个成员编码不同的 抗RhD重组多克隆抗体组成成员，位于所述集合中各个细胞基因组的相同位点。 在合适的条件下培养细胞以从细胞或培养上清液获得的重组多克隆抗体的表达。 编码抗RhD rpAb的核酸片段通过用载体文库进行转染而引入细胞，用于位点特异性整合。 该方法适用于制造抗RhD rpAb，从而使得等离子体衍生的预防和治疗性免疫球蛋白产品得到更好的替代。
-
公开(公告)号：US08198415B2
公开(公告)日：2012-06-12
申请号：US11632937
申请日：2005-07-18
Applicant: Søren Kofoed Rasmussen , Anne Bondgaard Tolstrup , Søren Bregenholt Frederiksen , John Haurum
Inventor： Søren Kofoed Rasmussen , Anne Bondgaard Tolstrup , Søren Bregenholt Frederiksen , John Haurum
IPC: C07K16/00
CPC classification number: C07K16/34 , C07K16/005 , C07K2317/21 , C07K2317/55 , C07K2317/77
Abstract: The invention relates to a method for manufacturing an anti-RhD recombinant polyclonal antibody composition (anti-RhD rpAb). The method comprises obtaining a collection of cells transfected with a library of anti-RhD antibody expression vectors, wherein each cell in the collection is capable of expressing from a VH and VL comprising nucleic acid segment, one member of the library, which encodes a distinct member of anti-RhD recombinant polyclonal antibody composition and which is located at the same site in the genome of individual cells in said collection. The cells are cultured under suitable conditions for expression of the recombinant polyclonal antibody, which is obtained from the cells or culture supernatant. The nucleic acid segments encoding the anti-RhD rpAb is introduced into the cells by transfection with a library of vectors for site-specific integration. The present method is suitable for manufacturing anti-RhD rpAb, thereby making available a superior replacement of plasma-derived prophylactic and therapeutic immunoglobulin products.
Abstract translation: 本发明涉及一种制备抗RhD重组多克隆抗体组合物（抗RhD rpAb）的方法。 该方法包括获得用抗RhD抗体表达载体文库转染的细胞的集合，其中集合中的每个细胞能够从包含核酸片段的VH和VL表达，文库的一个成员编码不同的 抗RhD重组多克隆抗体组合物的成员，并且位于所述收集物中各个细胞的基因组中的相同位点。 在合适的条件下培养细胞以从细胞或培养上清液获得的重组多克隆抗体的表达。 编码抗RhD rpAb的核酸片段通过用载体文库转染引入细胞，用于位点特异性整合。 本发明的方法适用于制造抗RhD rpAb，从而使得等离子体衍生的预防和治疗性免疫球蛋白产品更具优势。
-
公开(公告)号：US20090111142A1
公开(公告)日：2009-04-30
申请号：US12153809
申请日：2008-05-23
Applicant: Lars Soegaard Nielsen , Dietmar Weilguny , Anne Bondgaard Tolstrup , Finn C. Wiberg , Christian Muller
Inventor： Lars Soegaard Nielsen , Dietmar Weilguny , Anne Bondgaard Tolstrup , Finn C. Wiberg , Christian Muller
CPC classification number: C12N15/1086 , C07K16/00 , C07K16/081
Abstract: The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.
Abstract translation: 本发明涉及制备重组多克隆蛋白质组合物，特别是重组多克隆抗体组合物的方法。 该方法包括获得用变体核酸序列的集合转染的细胞的集合，其中集合中的每个细胞被转染并能够表达编码多克隆蛋白的不同成员的集合的一个成员。 细胞在合适的条件下培养，用于表达从细胞或培养物上清液获得的多克隆蛋白质。 通过用载体的集合转染将核酸序列引入细胞。 本发明方法适用于制备用于治疗用途的重组多克隆抗体。
-
6.发明申请公开(公告)号：US20090017017A1
公开(公告)日：2009-01-15
申请号：US11632937
申请日：2005-07-18
Applicant: Soren Kofoed Rasmussen , Anne Bondgaard Tolstrup , Soren Bregenholt Frederiksen , John Haurum
Inventor： Soren Kofoed Rasmussen , Anne Bondgaard Tolstrup , Soren Bregenholt Frederiksen , John Haurum
CPC classification number: C07K16/34 , C07K16/005 , C07K2317/21 , C07K2317/55 , C07K2317/77
Abstract: The invention relates to a method for manufacturing an anti-RhD recombinant polyclonal antibody composition (anti-RhD rpAb). The method comprises obtaining a collection of cells transfected with a library of anti-RhD antibody expression vectors, wherein each cell in the collection is capable of expressing from a VH and VL comprising nucleic acid segment, one member of the library, which encodes a distinct member of anti-RhD recombinant polyclonal antibody composition and which is located at the same site in the genome of individual cells in said collection. The cells are cultured under suitable conditions for expression of the recombinant polyclonal antibody, which is obtained from the cells or culture supernatant. The nucleic acid segments encoding the anti-RhD rpAb is introduced into the cells by transfection with a library of vectors for site-specific integration. The present method is suitable for manufacturing anti-RhD rpAb, thereby making available a superior replacement of plasma-derived prophylactic and therapeutic immonoglobulin products.
Abstract translation: 本发明涉及一种制备抗RhD重组多克隆抗体组合物（抗RhD rpAb）的方法。 该方法包括获得用抗RhD抗体表达载体文库转染的细胞的集合，其中集合中的每个细胞能够从包含核酸片段的VH和VL表达，文库的一个成员编码不同的 抗RhD重组多克隆抗体组合物的成员，并且位于所述收集物中各个细胞的基因组中的相同位点。 在合适的条件下培养细胞以从细胞或培养上清液获得的重组多克隆抗体的表达。 编码抗RhD rpAb的核酸片段通过用载体文库转染引入细胞，用于位点特异性整合。 本发明方法适用于制备抗RhD rpAb，从而使得等离子体衍生的预防和治疗性亚铁蛋白产品更具优势。
-
-
-
-
-
Search Results
6
records
(took 0.017 seconds)
