公开(公告)号：US20090004746A1

公开(公告)日：2009-01-01

申请号：US12131627

申请日：2008-06-02

申请人： Mana SRIYUDTHSAK ,  Apinan SOOTTITANTAWAT ,  Yongyuth WANNA ,  Sakon RAHONG ,  Vichuta LAURUENGTANA ,  Naoki ICHIKAWA ,  Sohei MATSUMOTO ,  Ryutaro MAEDA

发明人： Mana SRIYUDTHSAK ,  Apinan SOOTTITANTAWAT ,  Yongyuth WANNA ,  Sakon RAHONG ,  Vichuta LAURUENGTANA ,  Naoki ICHIKAWA ,  Sohei MATSUMOTO ,  Ryutaro MAEDA

IPC分类号： G01N33/00 ,  B01J19/00 ,  C12M1/40 ,  G01N21/01 ,  G01N25/00 ,  B32B38/10

CPC分类号： B01L3/502707 ,  B01L3/5027 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0887 ,  C12Q1/25

摘要： A microfluidic device allowing for multiple discrete reactions sites and allowing for sequential reactions and sample analysis along with methods for device fabrication and use is provided. The microfluidic device provides a micro-total analysis system on a single substrate and has multiple reaction sites allowing for cascade reactions and analysis on a single chip using micro-quantities of sample and reagents. The microfluidic device provides discrete sites for immobilization of cognitive agents including enzymes, binding proteins, nucleic acids and the like as well as methods for quantitative analysis. The invention also provides methods for the fabrication of the device.

摘要翻译： 提供了允许多个离散反应位点和允许顺序反应和样品分析以及用于器件制造和使用的方法的微流体器件。 微流体装置在单个底物上提供微量分析系统，并具有多个反应位点，允许使用微量的样品和试剂在单个芯片上进行级联反应和分析。 微流体装置提供用于固定认知剂（例如酶，结合蛋白，核酸等）的离散位点以及用于定量分析的方法。 本发明还提供了用于制造该装置的方法。