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    METABOLIC ENGINEERING OF YEASTS FOR THE PRODUCTION OF 1-BUTANOL
    2.
    发明申请
    METABOLIC ENGINEERING OF YEASTS FOR THE PRODUCTION OF 1-BUTANOL 审中-公开
    用于生产1-丁醇的代谢工程

    公开(公告)号：US20100159546A1

    公开(公告)日：2010-06-24

    申请号：US12593842

    申请日：2008-03-27

    申请人： Aristos Aristidou ,  Arlene M. Fosmer ,  Catherine Asleson Dundon ,  Brian J. Rush

    发明人： Aristos Aristidou ,  Arlene M. Fosmer ,  Catherine Asleson Dundon ,  Brian J. Rush

    IPC分类号： C12P7/16 ,  C12N1/19

    CPC分类号： C12N15/52 ,  C12P7/16 ,  Y02E50/10

    摘要： The present invention provides genetically modified yeast cells, and methods of using those yeast cells, to produce 1-butanol. The yeast cell can be selected from the genera Saccharomyces, Candida, Pichia, Kluyveromyces, Issatchenkia, Yarrowia, Rhodotorula, Hansenula, Schizochytrium, or Thraustochytrium. The yeast cell of the invention overexpresses at least one enzyme that catalyzes one or more butanoate pathways selected from the group consisting of pyruvate to acetyl-CoA, acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to 3-hydroxybutanoyl-CoA, 3-hydroxybutanoyl-CoA to crotonoyl-CoA, crotonoyl-CoA to butyryl-CoA to butyraldehyde, and butyraldehyde to 1-butanol. Some embodiments overexpress enzymes that are endogenous to the bacterium Clostridium acetobutylicum. The genetically modified yeast cell can further be subjected to other desired genetic changes, such as deletion or disruption of one or more native PDC genes.

    摘要翻译： 本发明提供遗传修饰的酵母细胞，以及使用这些酵母细胞的方法来生产1-丁醇。 酵母细胞可以选自酵母属，假丝酵母​​属，毕赤酵母属，克鲁维酵母属，发chen ia属，耶氏酵母属，红酵母属，汉逊酵母属，裂殖酵菌属或破囊壶菌属。 本发明的酵母细胞过表达至少一种催化一种或多种选自丙酮酸至乙酰辅酶A，乙酰辅酶A，乙酰乙酰辅酶A，乙酰乙酰辅酶A至3-羟基丁酰辅酶A， 羟基丁酰辅酶A，巴豆酰基-CoA，巴豆酰基-CoA至丁酰-CoA至丁醛，丁醛至丁醇。 一些实施方案过表达对细菌丙酮丁醇梭菌是内源的酶。 遗传修饰的酵母细胞可进一步经受其它所需的遗传变化，例如一个或多个天然PDC基因的缺失或破坏。