Electrobiochemical analytical method and electrodes
    2.
    发明授权
    Electrobiochemical analytical method and electrodes 失效
    电化学分析方法和电极

    公开(公告)号:US5443701A

    公开(公告)日:1995-08-22

    申请号:US109922

    申请日:1993-08-23

    IPC分类号: G01N27/327 C12Q1/00 G01N27/26

    摘要: An analytical method for determining the presence or concentration of an analyte in a liquid medium is provided in which electrons are transferred from an electrode material of an electrode to the redox center of an enzyme, by the mediation of an electron mediator, whereby in the presence of an analyte the enzyme calalyzed a redox reaction in which the analyte is converted into a product. By measuring either the concentration or the product or the charge which flows in this process, the presence and/or concentration of the analyte in the medium is determined. Either or both of the enzyme and the electron mediator are immobilized on the electrode.

    摘要翻译: 提供了一种用于确定液体介质中分析物的存在或浓度的分析方法,其中电子通过电子介体的介导从电极的电极材料转移到酶的氧化还原中心,由此在存在 的分析物,酶催化氧化还原反应,其中分析物转化成产物。 通过测量在该过程中流动的浓度或产物或电荷,确定介质中分析物的存在和/或浓度。 酶和电子介体中的任一个或两者被固定在电极上。