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    Expression System, Components Thereof and Methods of Use
    2.
    发明申请
    Expression System, Components Thereof and Methods of Use 有权
    表达系统及其组件及其使用方法

    公开(公告)号：US20080311618A1

    公开(公告)日：2008-12-18

    申请号：US11575621

    申请日：2005-10-03

    申请人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    发明人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12N15/11 ,  C07K14/00 ,  C12N5/10

    CPC分类号： C07K14/21

    摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putido. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.

    摘要翻译： 最近，诱导型表达系统的发展涉及从假单胞菌（Pseudomonas putido）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子例如枯烯或其等同物时表现出对DNA的增加的亲和力。 还公开了CymR变体，掺入这些变体的融合蛋白及其在多核苷酸的控制和表达中的用途。

    Reverse cumate repressor mutant
    3.
    发明授权
    Reverse cumate repressor mutant 有权
    反向并发阻遏物突变体

    公开(公告)号：US08728759B2

    公开(公告)日：2014-05-20

    申请号：US11575621

    申请日：2005-10-03

    申请人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    发明人： Yan Xu ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12N5/00 ,  C12N5/04 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/21

    摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.

    摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子例如枯烯或其等同物时表现出对DNA的增加的亲和力。 还公开了CymR变体，掺入这些变体的融合蛋白及其在多核苷酸的控制和表达中的用途。

    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
    5.
    发明授权
    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria 有权
    调节甲基营养和甲烷营养细菌中的异源重组蛋白表达

    公开(公告)号：US08216821B2

    公开(公告)日：2012-07-10

    申请号：US11990779

    申请日：2006-08-21

    申请人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    发明人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12N1/21 ,  C12N15/74

    CPC分类号： C12N15/74 ,  C12P21/02

    摘要： Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

    摘要翻译： 用目的基因转化甲基营养型或甲烷营养型细菌（如甲基杆菌属），并通过与所关心的基因有效连接的丙烯酸阻遏蛋白和操纵子序列来调节基因的表达，并加入外部药剂 。 具体来说，来自恶臭假单胞菌的cymR阻遏物和cmt操纵子可以用于调节甲基营养型或甲烷营养型细菌中添加有机物的基因表达。

    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
    8.
    发明申请
    Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria 有权
    调节甲基营养和甲烷营养细菌中的异源重组蛋白表达

    公开(公告)号：US20100221813A1

    公开(公告)日：2010-09-02

    申请号：US11990779

    申请日：2006-08-21

    申请人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    发明人： Carlos B. Miguez ,  Young-Jun Choi ,  Alaka Mullick ,  Bernard Massie

    IPC分类号： C12N1/21 ,  C12N15/74

    CPC分类号： C12N15/74 ,  C12P21/02

    摘要： Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

    摘要翻译： 用目的基因转化甲基营养型或甲烷营养型细菌（如甲基杆菌属），并通过与所关心的基因有效连接的丙烯酸阻遏蛋白和操纵子序列来调节基因的表达，并加入外部药剂 。 具体来说，来自恶臭假单胞菌的cymR阻遏物和cmt操纵子可以用于调节甲基营养型或甲烷营养型细菌中添加有机物的基因表达。

    Reverse cumate repressor mutant
    9.
    发明授权
    Reverse cumate repressor mutant 有权
    反向并发阻遏物突变体

    公开(公告)号：US07935788B2

    公开(公告)日：2011-05-03

    申请号：US12331786

    申请日：2008-12-10

    申请人： Félix Malenfant ,  Alaka Mullick

    发明人： Félix Malenfant ,  Alaka Mullick

    IPC分类号： C07K14/00

    CPC分类号： C07K14/21

    摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.

    摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子如枯烯或其等同物时表现出对DNA的增加的亲和力 。 还公开了CymR变体，掺入这种变体的融合蛋白及其在多核苷酸的控制和表达中的用途。 CymR变体包含野生型CymR的142Glu至142Gly单点突变。

    Reverse cumate repressor mutant
    10.
    发明授权
    Reverse cumate repressor mutant 有权
    反向并发阻遏物突变体

    公开(公告)号：US08252556B2

    公开(公告)日：2012-08-28

    申请号：US13070975

    申请日：2011-03-24

    申请人： Felix Malenfant ,  Alaka Mullick

    发明人： Felix Malenfant ,  Alaka Mullick

    IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N5/02 ,  C12N5/10 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C07K14/21

    摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.

    摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子如枯烯或其等同物时表现出对DNA的增加的亲和力 。 还公开了CymR变体，掺入这种变体的融合蛋白及其在多核苷酸的控制和表达中的用途。 CymR变体包含野生型CymR的142Glu至142Gly单点突变。