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1.发明申请HERBICIDE-RESISTANT RICE PLANTS, POLYNUCLEOTIDES ENCODING HERBICIDE RESISTANT ACETOHYDROXYACID SYNTHASE LARGE SUBUNIT PROTEINS, AND METHODS OF USE 审中-公开抗除草剂植物，编码抗除草剂乙酰氧基合成酶大分子蛋白的多核苷酸及其使用方法公开(公告)号：US20120233723A1
公开(公告)日：2012-09-13
申请号：US13418018
申请日：2012-03-12
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00 , A01P13/00 , A01C1/06 , C12N5/10 , A01N43/50 , C12N15/29 , A01H5/10
CPC分类号： C12N9/1022 , C12N9/88 , C12N15/8278
摘要： Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxyacid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.
摘要翻译： 描述除草剂抗性水稻植物，编码除草剂抗性和野生型乙酰羟基酸合成酶大亚基1（AHASL1）多肽的分离的多核苷酸，以及这些多肽的氨基酸序列。 描述了包含本发明多核苷酸的表达盒和转化载体，以及用多核苷酸转化的植物和宿主细胞。 还描述了使用多核苷酸增强植物对咪唑啉酮除草剂的抗性的方法，以及控制除草剂抗性植物附近杂草的方法。
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公开(公告)号：US08206906B2
公开(公告)日：2012-06-26
申请号：US12205246
申请日：2008-09-05
申请人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦
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3.发明申请HERBICIDE-RESISTANT SUNFLOWER PLANTS WITH MULTIPLE HERBICIDE RESISTANT ALLELES OF AHASL1 AND METHODS OF USE 审中-公开具有多种抗AHASL1抗性抗性的抗除草剂植物和使用方法公开(公告)号：US20110277051A1
公开(公告)日：2011-11-10
申请号：US12594289
申请日：2008-04-02
申请人： Carlos Sala , Mariano Bulos , Sherry R. Whitt , Brigitte J. Weston , Adriana Mariel Echarte , Bijay K. Singh
发明人： Carlos Sala , Mariano Bulos , Sherry R. Whitt , Brigitte J. Weston , Adriana Mariel Echarte , Bijay K. Singh
CPC分类号： C12N15/8274 , A01H1/00 , A01H5/02 , A01H6/14 , A01N43/50 , C12N9/88 , C12N15/8278
摘要： Herbicide resistant sunflower plants comprising two different herbicide-resistant alleles of the sunflower acetohydroxyacid synthase large subunit 1 (AHASL1) gene are described. Methods for making these sunflower plants and methods for controlling weeds or other undesired vegetation growing in the vicinity of these sunflower plants are disclosed. Such methods involve the use of acetohydroxyacid synthase-inhibiting herbicides. Methods for controlling parasitic weeds growing on sunflower plants are also described. Additionally provided are methods for determining the genotype of sunflower plants for AHASL1 gene.
摘要翻译： 描述了包含向日葵乙酰羟酸合成酶大亚基1（AHASL1）基因的两种不同的除草剂抗性等位基因的除草剂抗性向日葵植物。 公开了制造这些向日葵植物的方法和控制在这些向日葵植物附近生长的杂草或其他不期望的植物的方法。 这些方法涉及使用乙酰羟酸合成酶抑制性除草剂。 还描述了控制向日葵植物生长的寄生杂草的方法。 另外提供了用于确定向日葵植物对AHASL1基因的基因型的方法。
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公开(公告)号：US20090098552A1
公开(公告)日：2009-04-16
申请号：US12205312
申请日：2008-09-05
申请人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦
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公开(公告)号：US20090029381A1
公开(公告)日：2009-01-29
申请号：US12205246
申请日：2008-09-05
申请人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
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公开(公告)号：US07432082B2
公开(公告)日：2008-10-07
申请号：US10805973
申请日：2004-03-22
申请人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦
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7.发明授权
公开(公告)号：US5202251A
公开(公告)日：1993-04-13
申请号：US560677
申请日：1990-08-03
摘要： This invention is directed to the generation of hybridomas and the monocot-specific monoclonal antibodies prepared from those hybridomas against the acetohydroxyacid synthase enzyme. The monoclonal antibodies are useful to isolate and purify the enzyme. The invention is also directed to peptides corresponding to fragments of the enzyme which then are used to generate the monoclonal antibodies.
摘要翻译： 本发明涉及从针对乙酰羟酸合酶的那些杂交瘤制备的杂交瘤和单子叶特异性单克隆抗体的产生。 单克隆抗体可用于分离和纯化酶。 本发明还涉及对应于酶的片段的肽，然后用于产生单克隆抗体。
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公开(公告)号：US20130289264A1
公开(公告)日：2013-10-31
申请号：US13527103
申请日：2012-06-19
申请人： Chengyan ZHAO , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan ZHAO , Robert Ascenzi , Bijay K. Singh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦
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9.发明申请METHODS FOR MAKING AND USING WHEAT PLANTS WITH INCREASED GRAIN PROTEIN CONTENT 审中-公开用于制备和使用具有增加的谷蛋白含量的小麦植物的方法公开(公告)号：US20090300783A1
公开(公告)日：2009-12-03
申请号：US12302101
申请日：2007-05-31
CPC分类号： C12N15/8251 , A01H5/10
摘要： The present invention provides novel methods for making wheat plants with increased grain protein content. The methods involve introducing a gene encoding herbicide-resistant, wheat acetohydroxyacid synthase large subunit (AHASL) protein. The invention further provides wheat plants that produce high protein grain and human and animal food products derived thereof.
摘要翻译： 本发明提供了制备具有增加的谷物蛋白质含量的小麦植物的新方法。 该方法涉及引入编码除草剂抗性小麦乙酰羟酸合成酶大亚基（AHASL）蛋白的基因。 本发明还提供了产生高蛋白质谷物的小麦植物和源自其的人畜动物食品。
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公开(公告)号：US08877911B2
公开(公告)日：2014-11-04
申请号：US13527103
申请日：2012-06-19
申请人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
发明人： Chengyan Zhao , Robert Ascenzi , Bijay K. Singh
CPC分类号： C12Q1/6876 , C12Q1/6895 , C12Q2600/156
摘要： The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
摘要翻译： 本发明涉及用于分析植物乙酰羟酸合酶大亚基（AHASL）基因的方法和组合物。 特别地，本发明涉及检测野生型AHASL等位基因的方法和编码咪唑啉酮耐受性AHASL蛋白的突变型AHASL等位基因。 所述方法包括使用PCR扩增和包含等位基因特异性和基因特异性引物的新型组合来检测存在于植物个体AHASL基因的突变体和/或野生型等位基因的存在。 具体地说，该方法和组合物可用于分析小麦Triticum aestivum的三种AHASL基因和Twiticum turgidum ssp的两种AHASL基因。 硬粒小麦
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